摘要：【中文】本发明公开了蛋白质ZmPT3在调控植物磷含量中的应用。向玉米自交系B73中导入蛋白质ZmPT3的编码基因，得到转基因玉米OE‑1和OE‑2。在幼苗期，与玉米自交系B73相比，OE‑1和OE‑2的幼叶和茎的磷含量和生物量均显著增加；在散粉期，与玉米自交系B73相比，OE‑1和OE‑2的穗位叶和雌穗的磷含量显著增加。因此，蛋白质ZmPT对于进一步阐明植物磷营养的分子机理并通过基因工程的技术手段培育磷营养高效的作物新品种具有重要的理论意义和实践意义。本发明具有重大的应用价值。 【EN】The invention discloses application of protein ZmPT3 in regulation and control of phosphorus content in plants. The coding gene of protein ZmPT3 is introduced into a maize inbred line B73 to obtain transgenic maize OE-1 and OE-2. At the seedling stage, compared with the maize inbred line B73, the phosphorus content and biomass of the young leaves and stems of OE-1 and OE-2 are both significantly increased; in the pollen-dispersing period, the phosphorus content of ear position leaves and female ears of OE-1 and OE-2 is obviously increased compared with that of the maize inbred line B73. Therefore, the protein ZmPT has important theoretical significance and practical significance for further clarifying the molecular mechanism of plant phosphorus nutrition and cultivating new crop varieties with high phosphorus nutrition efficiency by a technical means of genetic engineering. The invention has great application value.

摘要：【中文】本发明提出了微生物、培养微生物的方法和食品或药物。所述微生物为双歧杆菌(Bifidobacterium animalis subsp.lactis A6)，于2014年6月5日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC NO.9273。本发明的微生物具有分解利用人乳低聚糖的能力，可以人乳低聚糖作为碳源，尤其是可以人乳低聚糖作为唯一碳源生长。由此，为人乳低聚糖、动物双歧杆菌以及食品或药品中益生菌和人乳低聚糖的添加等提供了理论依据。 【EN】The invention provides a microorganism, a method for culturing the microorganism and a food or a medicament. The microorganism is Bifidobacterium bifidum (Bifidobacterium animalis subsp. lactis A6), is preserved in China general microbiological culture collection center (CGMCC NO. 9273) in 6-5 th month in 2014, and has a preservation number of CGMCC NO. 9273. The microorganism of the invention has the capability of decomposing and utilizing the human milk oligosaccharide, can grow by taking the human milk oligosaccharide as a carbon source, and particularly can grow by taking the human milk oligosaccharide as a unique carbon source. Therefore, theoretical basis is provided for the addition of the human milk oligosaccharide, the animal bifidobacterium, the probiotics and the human milk oligosaccharide in the food or the medicine, and the like.

摘要：【中文】本发明涉及工程计算水力学，提供了一种基于时间尺度的旋转湍流涡粘阻尼的计算方法，包括：根据旋转湍流的特征频率获取解析时间尺度、模化时间尺度和相对螺旋度；根据解析时间尺度、模化时间尺度和相对螺旋度，获取旋转湍流的涡粘阻尼。本发明提供的基于时间尺度的旋转湍流涡粘阻尼的计算方法，涡粘阻尼的计算无需联系网格空间尺度，有利于保证数值计算的高效性和鲁棒性；新的时间尺度经验表达式能直接适应强瞬态、高脉动、大分离的流动工况；明确考虑了水力机械内显著存在的能量反级串输运现象。该计算方法在应用中能在适应流动特点的同时更好地兼顾计算精度与计算成本，可为水力机械内旋转湍流的高效解析提供新思路。 【EN】The invention relates to engineering computational hydraulics, and provides a method for calculating rotary turbulent vortex-viscous damping based on time scale, which comprises the following steps: acquiring an analysis time scale, a modeling time scale and relative helicity according to the characteristic frequency of the rotating turbulence; and acquiring vortex-viscous damping of the rotating turbulence according to the analysis time scale, the modeling time scale and the relative helicity. According to the method for calculating the vortex-viscous damping of the rotating turbulence based on the time scale, the calculation of the vortex-viscous damping does not need to be connected with the grid space scale, and the high efficiency and robustness of numerical calculation are guaranteed; the new time scale empirical expression can be directly adapted to the flow working conditions of strong transient state, high pulsation and large separation; the phenomenon of reverse cascade transport of energy which obviously exists in the hydraulic machine is explicitly considered. The calculation method can be applied to the flow characteristics, better considers the calculation precision and the calculation cost, and can provide a new idea for efficient analysis of the rotating turbulence in the hydraulic machinery.

摘要：【中文】本发明提供一种配电网灾前资源配置方法及系统，方法包括：建立基于移动储能装置的极端灾害下配电网安全性优化子目标模型和常态运行下配电网经济性优化子目标模型；根据配电网安全性优化子目标模型和所述配电网经济性优化子目标模型，建立基于纳什谈判的移动储能装置配置模型；对移动储能装置配置模型求解，得到移动储能装置容量配置的均衡解。本发明能够满足配电网的安全性要求和经济性要求，且解决了安全性和经济性优化子目标之间的矛盾，避免了以大量投资换取极端灾害小概率场景下安全运行的不足，能够在提升极端灾害下配电网韧性的同时大幅提高常态运行的经济效益。 【EN】The invention provides a method and a system for allocating pre-disaster resources of a power distribution network, wherein the method comprises the following steps: establishing a sub-objective model for optimizing the safety of the power distribution network under extreme disasters and a sub-objective model for optimizing the economy of the power distribution network under normal operation based on the mobile energy storage device; establishing a mobile energy storage device configuration model based on Nash negotiation according to a distribution network safety optimization sub-objective model and a distribution network economic optimization sub-objective model; and solving the mobile energy storage device configuration model to obtain an equilibrium solution of the mobile energy storage device capacity configuration. The method can meet the safety requirement and the economic requirement of the power distribution network, solves the contradiction between the safety and economic optimization sub-targets, avoids the defect that the power distribution network runs safely under the condition of small probability of extreme disasters by replacing a large amount of investment, and can greatly improve the economic benefit of normal running while improving the toughness of the power distribution network under the extreme disasters.

摘要：【中文】本发明提供一种乳腺特异性表达抗菌肽Cecropin基因的同源重组载体及应用，所述同源重组载体是以真核表达载体为骨架载体，重组载体中至少包括正筛选元件、负筛选标记基因、抗菌肽Cecropin基因表达盒以及rosa26基因的同源左臂和同源右臂；其中，正筛选元件包括顺次连接的重组酶识别序列‑正筛选标记基因‑重组酶基因‑重组酶识别序列。利用所述同源重组载体转染哺乳动物体细胞，获得的阳性细胞rosa26基因第一个内含子定点整合抗菌肽Cecropin基因。利用本发明方法成功获得了乳腺特异性表达抗菌肽Cecropin的转基因猪细胞系，为通过转基因技术制备抗腹泻转基因猪奠定基础。 【EN】The invention provides a homologous recombination vector for specifically expressing an antibacterial peptide Cecropin gene in mammary gland and application, wherein the homologous recombination vector takes a eukaryotic expression vector as a skeleton vector, and at least comprises a positive screening element, a negative screening marker gene, an antibacterial peptide Cecropin gene expression box, and a homologous left arm and a homologous right arm of a rosa26 gene; wherein the positive screening element comprises a recombinase recognition sequence-positive screening marker gene-recombinase recognition sequence which are connected in sequence. The homologous recombination vector is used for transfecting mammalian somatic cells, and the antibacterial peptide Cecropin gene is integrated into the first intron of the rosa26 gene of the positive cell at a fixed point. The method successfully obtains the transgenic pig cell line of the mammary gland specificity expression antibacterial peptide Cecropin, and lays a foundation for preparing the diarrhea-resistant transgenic pig by a transgenic technology.

摘要：【中文】本发明实施例提供一种客户分类方法、系统以及电子设备，该方法包括：获取由多个混合数据样本构成的混合型数据样本集；每个混合数据样本由数值型变量样本和分类型变量样本组成；对数值型变量样本进行特征提取，获取综合评价因子；获取综合评价因子和分类型变量样本的相异性度量值；基于粒子群优化算法，对相异性度量值的K‑Means聚类算法进行优化，构建客户分类模型，进而获取客户分类结果。本发明实施例结合K‑Means聚类算法和改进的粒子群优化算法，考虑多种影响客户消费的指标，采用优化聚类算法对客户消费数据进行聚类分析，得到具有不同特征的客户群组，分析结果更合理准确，便于对不同群组采取不同的经营和客户服务策略。 【EN】The embodiment of the invention provides a customer classification method, a customer classification system and electronic equipment, wherein the method comprises the following steps: acquiring a mixed data sample set consisting of a plurality of mixed data samples; each mixed data sample consists of a numerical type variable sample and a classification type variable sample; carrying out feature extraction on the numerical variable sample to obtain a comprehensive evaluation factor; acquiring dissimilarity measurement values of the comprehensive evaluation factors and the classified variable samples; and optimizing the K-Means clustering algorithm of the dissimilarity metric value based on a particle swarm optimization algorithm, constructing a customer classification model, and further obtaining a customer classification result. The embodiment of the invention combines the K-Means clustering algorithm and the improved particle swarm optimization algorithm, considers various indexes influencing customer consumption, adopts the optimization clustering algorithm to perform clustering analysis on customer consumption data to obtain customer groups with different characteristics, has more reasonable and accurate analysis results, and is convenient for adopting different operation and customer service strategies for different groups.

摘要：【中文】本发明实施例提供一种猪的品种溯源方法及装置，该方法包括：获取猪基因数据集；对所述猪基因数据集进行分析，得到有效祖先信息标记数据；根据所述有效祖先信息标记数据进行群体分类分析，得到群体分类目标祖先信息标记数据，以根据所述群体分类目标祖先信息标记数据进行猪种品种溯源。通过针对中国东部猪基因数据信息、中国南部猪基因数据信息和欧洲商品猪基因数据信息进行群体分类分析，得到能够实现对于中国东部猪、中国南部猪和欧洲商品猪实现品种溯源的群体分类目标祖先信息标记数据，且本申请中群体分类目标祖先信息标记数据的数量较小，能够准确高效的实现对于猪的品种溯源。 【EN】The embodiment of the invention provides a pig variety tracing method and a pig variety tracing device, wherein the method comprises the following steps: acquiring a pig gene data set; analyzing the pig gene data set to obtain effective ancestor information marking data; and performing group classification analysis according to the effective ancestor information marking data to obtain group classification target ancestor information marking data, and tracing the source of the pig breed according to the group classification target ancestor information marking data. Group classification analysis is carried out on Chinese eastern pig gene data information, Chinese southern pig gene data information and European commodity pig gene data information, group classification target ancestor information marking data capable of realizing variety tracing of Chinese eastern pigs, Chinese southern pigs and European commodity pigs are obtained, the number of the group classification target ancestor information marking data is small, and variety tracing of pigs can be accurately and efficiently realized.

摘要：【中文】本发明涉及一种高通量检测用于预测籼稻亚种穗粒数强优势杂交组合的SNP标记的方法。本发明还涉及用于预测籼稻亚种穗粒数强优势杂交组合的标签SNP以及用于检测或扩增核心标签SNP的引物组。本发明进一步涉及评估和预测籼稻穗粒数强优势杂交组合的方法以及本发明的标签SNP和引物组在评估和预测籼稻穗粒数强优势杂交组合中的应用。 【EN】The invention relates to a high-throughput detection method for predicting SNP markers of dominant hybridization combinations with strong spike grain number of indica rice subspecies. The invention also relates to a label SNP for predicting the dominant hybridization combination of the high ear number of the indica rice subspecies and a primer group for detecting or amplifying the core label SNP. The invention further relates to a method for evaluating and predicting the indica rice grain number strong dominant hybridization combination and application of the label SNP and the primer set in evaluating and predicting the indica rice grain number strong dominant hybridization combination.

摘要：【中文】本发明涉及一种基于CAN总线的玉米收获机智能控制试验平台，包括试验台主体，以及设置在试验台主体上的参数显示设置面板、整车控制器、收获控制器、演示面板、试验台操作模块、模拟执行机构和测量变送装置；参数显示设置面板、整车控制器和收获控制器通过CAN总线连接；收获控制器接收和存储待模拟调试的玉米收获机控制系统的控制模块，根据参数显示设置面板所设置的收获参数和控制模块对整车控制器发送控制指令，对收获过程进行自动模拟调节；试验台操作模块与整车控制器的输入端口连接；演示面板上绘制有玉米收获机模拟图；模拟执行机构包括多个分别与整车控制器的输出端口连接的模拟执行部件；测量变送装置采集各模拟执行部件的数据信号。 【EN】The invention relates to a CAN bus-based intelligent control test platform of a corn harvester, which comprises a test bed main body, a parameter display setting panel, a whole vehicle controller, a harvesting controller, a demonstration panel, a test bed operation module, a simulation execution mechanism and a measurement transmitting device, wherein the parameter display setting panel, the whole vehicle controller, the harvesting controller, the demonstration panel, the test bed operation module, the simulation execution mechanism and the measurement transmitting device are arranged on the test bed main body; the parameter display setting panel, the whole vehicle controller and the harvesting controller are connected through a CAN bus; the harvesting controller receives and stores a control module of a corn harvester control system to be simulated and debugged, and sends a control instruction to the whole vehicle controller according to the harvesting parameters and the control module set by the parameter display setting panel to perform automatic simulation adjustment on the harvesting process; the test bed operation module is connected with an input port of the whole vehicle controller; a simulation diagram of the corn harvester is drawn on the demonstration panel; the simulation execution mechanism comprises a plurality of simulation execution components which are respectively connected with the output port of the whole vehicle controller; the measuring and transmitting device collects data signals of the analog execution components.

摘要：【中文】本发明提供非洲猪瘟CTL表位多肽及其应用，属于多肽疫苗制备技术领域。本发明通过生物信息学方法及鉴定MHC I结合基序的方法筛选来源于非洲猪瘟病毒(ASFV)中MHC I能够稳定结合的多肽，筛选得到的多肽具有能够启动CD8⁺T细胞产生免疫应答，并刺激机体特异性产生CTL的能力，所述多肽的氨基酸序列如SEQ ID NO.1‑18任一所示。本发明提供的多肽可用于制备非洲猪瘟特异性抗原表位疫苗或多肽疫苗，安全性高，特异性好，在非洲猪瘟的防控工作中具有潜在的应用价值。 【EN】The invention provides an African swine fever CTL epitope polypeptide and application thereof, belonging to the technical field of polypeptide vaccine preparation. The present invention relates to the identification of MHC by bioinformatics methodMethod for screening polypeptide capable of stably binding MHC I in African Swine Fever Virus (ASFV) and screening polypeptide capable of promoting CD8⁺T cells generate immune response and stimulate the capability of an organism to specifically generate CTL, and the amino acid sequence of the polypeptide is shown in any one of SEQ ID NO. 1-18. The polypeptide provided by the invention can be used for preparing an African swine fever specific epitope vaccine or polypeptide vaccine, has high safety and good specificity, and has potential application value in the prevention and control work of African swine fever.