摘要：【中文】本发明属于植物基因工程技术领域，具体涉及利用油菜BnaA6YUC6基因改良油菜分枝角度。本发明利用过表达油菜BnaA06YUC6基因的载体转化油菜，用以改良油菜的分枝角度，其中，所述油菜BnaA06YUC6基因的核苷酸序列如序列表SEQ ID NO：1所示；其蛋白质序列如序列表SEQ ID NO:2所示。以甘蓝型油菜品种中双6号为受体，构建过表达油菜BnaA06YUC6基因的转基因植株。功能验证表明，油菜BnaA06YUC6基因所表现出来的这种功能可以用来改良农作物的分枝结构，如减小分枝角度等性状。 【EN】The invention belongs to the technical field of plant genetic engineering, and particularly relates to a method for improving rape branch angle by utilizing rape BnaA6YUC6 gene. The invention utilizes a vector for over-expressing rape BnaA06YUC6 gene to transform rape so as to improve the branching angle of rape, wherein the nucleotide sequence of the rape BnaA06YUC6 gene is shown as a sequence table SEQ ID NO:1 is shown in the specification; the protein sequence is shown in a sequence table SEQ ID NO. 2. A transgenic plant of over-expression rape BnaA06YUC6 gene is constructed by taking double No. 6 in cabbage type rape variety as a receptor. Functional verification shows that the function expressed by the rape BnaA06YUC6 gene can be used for improving the branch structure of crops, such as reducing the characters of branch angle and the like.

摘要：【中文】本发明涉及一种纤维素凝胶微球固定化磷脂酶，以纤维素和纳米纤维素混合溶解、再生后的纤维素凝胶微球为载体，磷脂酶通过交联剂共价交联在纤维素凝胶微球上；其中所述交联剂包括N‑（3‑二甲氨基丙基）‑N'‑乙基碳二亚胺盐酸盐和N‑羟基琥珀酰亚胺。本发明所述纤维素凝胶微球固定化磷脂酶在不减少酶与反应液接触位点的同时，提高了酶的稳定性。 【EN】The invention relates to a cellulose gel microsphere immobilized phospholipase, which takes cellulose and nano-cellulose mixed and dissolved and regenerated cellulose gel microspheres as a carrier, and the phospholipase is covalently crosslinked on the cellulose gel microspheres through a crosslinking agent; wherein the crosslinking agent comprises N- (3-dimethylaminopropyl) -N' -ethylcarbodiimide hydrochloride and N-hydroxysuccinimide. The cellulose gel microsphere immobilized phospholipase provided by the invention improves the stability of enzyme without reducing the contact sites of the enzyme and reaction liquid.

摘要：【中文】本发明涉及二乙酰镳草镰刀菌烯醇流动滞后免疫时间分辨荧光速测试剂盒。它包括荧光试纸条和含有铕标记的抗二乙酰镳草镰刀菌烯醇单克隆抗体冻干品的样品反应瓶，其中：所述的荧光试纸条包括衬板，衬板的一面从上到下依次粘贴吸水垫、检测垫和样品垫，相邻各垫在连接处交叠连接，所述检测垫以硝酸纤维素膜为基垫，硝酸纤维素膜上自上而下横向设置质控线和检测线，所述质控线包被有兔抗鼠多克隆抗体，所述检测线上包被二乙酰镳草镰刀菌烯醇完全抗原(DAS‑OVA)。可准确、快速的测定二乙酰镳草镰刀菌烯醇含量。 【EN】The invention relates to a quick detection kit for flow lag immune time resolution fluorescence of sickle knife enol of ribes diacetylenii. It includes fluorescence test paper strip and contains the sample reaction bottle of the anti diacetyl grass fusarium enol monoclonal antibody freeze-dried product of europium mark, wherein: fluorescence test paper strip include the welt, the one side from the top down of welt pastes water absorption pad, detection pad and sample pad in proper order, and adjacent each pad is in junction overlap connection, detect and fill up and use the nitrocellulose membrane as the base pad, top-down transversely sets up quality control line and detection line on the nitrocellulose membrane, the quality control line peridium has rabbit anti mouse polyclonal antibody, peridium is diacetyl grass fusarium enol complete antigen (DAS-OVA) on the detection line. Can accurately and quickly determine the content of the diacetyl ribes sickle knife enol.

摘要：【中文】本发明涉及同步检测二乙酸镳草镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、T‑2毒素的时间分辨荧光试剂盒。它包括免疫层析时间分辨荧光试纸条和含有铕标记的各毒素的单克隆抗体冻干品的样品反应瓶，其中：所述的免疫层析时间分辨荧光试纸条包括衬板，衬板的一面从上到下依次粘贴吸水垫、检测垫和样品垫，相邻各垫在连接处交叠连接，所述检测垫以硝酸纤维素膜为基垫，硝酸纤维素膜上自上而下设置横向质控线和检测线，所述质控线包被有兔抗鼠多克隆抗体，所述检测线位于质控线下方，个数为3条，检测线上分别上包被各毒素蛋白偶联物。其能在一条试纸条上实现对二乙酸镳草镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、T‑2毒素三种真菌毒素的同步、快速检测。 【EN】The invention relates to a time-resolved fluorescence kit for synchronously detecting sickle knife fungus enol diacetate, deoxynivalenol and T-2 toxin. It comprises an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing monoclonal antibody freeze-dried products of various toxins marked by europium, wherein: the immunochromatography time-resolved fluorescence test strip comprises a lining plate, wherein a water absorption pad, a detection pad and a sample pad are sequentially pasted on one surface of the lining plate from top to bottom, the adjacent pads are in overlapped connection at the connection part, the detection pad takes a nitrocellulose membrane as a base pad, a transverse quality control line and a detection line are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit anti-mouse polyclonal antibody, the detection lines are positioned below the quality control line, the number of the detection lines is 3, and each toxin protein conjugate is coated on each detection line respectively. The method can realize synchronous and rapid detection of three fungaltoxins, namely sickle enol diacetate, deoxynivalenol and T-2 toxin, on one test strip.

摘要：【中文】本发明涉及二乙酸镳草镰刀菌烯醇竞争型气压免疫传感器及检测方法。二乙酸镳草镰刀菌烯醇竞争型气压免疫传感器，包括单独存放的抗二乙酸镳草镰刀菌烯醇单克隆抗体、酶标条、包被酶标条用二乙酸镳草镰刀菌烯醇‑半谷氨酸‑乳清蛋白偶联物、过氧化氢溶液、表面修饰羊抗鼠抗体的铂纳米粒子或金核铂壳纳米粒子和用于检测气压的气压检测传感器；所述抗二乙酸镳草镰刀菌烯醇单克隆抗体由保藏编号为CCTCC NO:C201881的杂交瘤细胞菌株DAS5G11E7产生。该方法具有灵敏度高、便携、廉价和易于操作等优点。 【EN】The invention relates to a ribes diacetate sickle enol competition type air pressure immunosensor and a detection method. The ribes diacetate sickle knife enol competitive type air pressure immunosensor comprises a ribes diacetate sickle enol monoclonal antibody, an enzyme label strip, a ribes diacetate sickle enol-half glutamic acid-lactalbumin conjugate for coating the enzyme label strip, a hydrogen peroxide solution, platinum nanoparticles or gold core platinum shell nanoparticles with surface modified goat anti-mouse antibodies and an air pressure detection sensor for detecting air pressure, wherein the ribes diacetate sickle enol monoclonal antibody is independently stored; the anti-ribes diacetate fusarenol monoclonal antibody is generated by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO: C201881. The method has the advantages of high sensitivity, portability, low cost, easy operation and the like.

摘要：【中文】本发明涉及同步检测二乙酸镳草镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、T‑2毒素胶体金免疫层析试纸条。它包括底板，底板的一面从上到下依次粘贴吸水垫、检测垫、金标垫和样品垫，相邻各垫在连接处交叠连接，检测垫以硝酸纤维素膜为基垫，硝酸纤维素膜上横向设置有质控线和检测线，检测线位于质控线的下方，个数为三条，呈间隔分布，三条检测线上分别包被有各毒素蛋白偶联物，所述质控线包被有兔抗鼠多克隆抗体；金标垫横向喷涂有胶体金标记的各抗体；所述抗二乙酸镳草镰刀菌烯醇单克隆抗体由保藏编号为CCTCC NO:C201881的杂交瘤细胞株DAS5G11E7分泌产生。能在一条试纸条上实现对二乙酸镳草镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、T‑2毒素三种真菌毒素的同步、快速检测。 【EN】The invention relates to a colloidal gold immunochromatographic test strip for synchronously detecting sickle knife enol, deoxynivalenol and T-2 toxin of ribes diacetate. The detection pad takes a nitrocellulose membrane as a base pad, quality control lines and detection lines are transversely arranged on the nitrocellulose membrane, the detection lines are positioned below the quality control lines, the number of the detection lines is three, the detection lines are distributed at intervals, each toxin protein conjugate is respectively coated on each detection line, and the quality control lines are coated with rabbit anti-mouse polyclonal antibodies; the gold label pad is transversely sprayed with each antibody marked by colloidal gold; the anti-ribes diacetate fusarenol monoclonal antibody is secreted and generated by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO: C201881. The method can realize synchronous and rapid detection of three fungaltoxins, namely sickle enol diacetate, deoxynivalenol and T-2 toxin, on one test strip.

摘要：【中文】本发明提供了一种双T‑DNA载体pBID‑RT Enhanced及其构建方法和应用，涉及转基因技术领域；所述载体以pCAMBIA3300的质粒为基础，包含两个反向独立的T‑DNA区，第一T‑DNA区的核苷酸序列如SEQ ID NO.1所示，第二T‑DNA区的核苷酸序列如SEQ ID NO.2所示。利用所述载体可降低T‑DNA整合到连锁位点的频率，T₁代和F₁代中无标记转基因植株占76.92％；利用所述载体可获得抗草铵膦转基因油菜以及既抗草铵膦又抗草甘膦转基因油菜。利用本发明的所述载体可以成功并且高效的生产无标记转基因甘蓝型油菜植株，并创造两种抗除草剂品种。 【EN】The invention provides a double T-DNA carrier pBID-RT Enhanced and a construction method and application thereof, relating to the technical field of transgenosis; the vector is based on a plasmid of pCAMBIA3300 and comprises two reverse independent T-DNA regions, wherein the nucleotide sequence of the first T-DNA region is shown as SEQ ID NO.1, and the nucleotide sequence of the second T-DNA region is shown as SEQ ID NO. 2. The use of the vector can reduce the frequency of integration of T-DNA into linked sites, T₁Generation and F₁The unmarked transgenic plants in the generation account for 76.92 percent; the glufosinate-resistant transgenic rape and the glufosinate-resistant and glyphosate-resistant transgenic rape can be obtained by utilizing the vector. The use of the vectors of the invention allows successful and efficient production of label-free productsTransgenic brassica napus plants and create two herbicide resistant varieties.

摘要：【中文】本发明提供一种2,6‑二甲氧基‑4‑乙烯基苯酚二聚物的制备方法，涉及食品化学合成技术领域。本发明提供的合成方法包括以下步骤：将芥子酸和酸溶液混合，进行回流反应，得到回流反应产物；将所述回流反应产物依次进行有机溶剂萃取、有机相洗涤、除水和干燥，得到浓缩物；将所述浓缩物溶解后，在液相色谱中进行洗脱，得到洗脱液；将所述洗脱液冷冻干燥，得到2,6‑二甲氧基‑4‑乙烯基苯酚二聚物。本发明以芥子酸为原料，在酸溶液的催化作用下，一步合成高纯度的2,6‑二甲氧基‑4‑乙烯基苯酚二聚物。且本发明提供的合成方法操作简便、易实现。 【EN】The invention provides a preparation method of 2,6-dimethoxy-4-vinylphenol dimer, and relates to the technical field of food chemical synthesis. The synthesis method provided by the invention comprises the following steps: mixing sinapic acid and an acid solution, and carrying out reflux reaction to obtain a reflux reaction product; sequentially carrying out organic solvent extraction, organic phase washing, water removal and drying on the reflux reaction product to obtain a concentrate; dissolving the concentrate, and eluting in a liquid chromatogram to obtain an eluent; and (3) freeze-drying the eluent to obtain the 2,6-dimethoxy-4-vinylphenol dimer. The invention takes sinapic acid as a raw material, and synthesizes the 2,6-dimethoxy-4-vinylphenol dimer with high purity in one step under the catalysis of acid solution. The synthesis method provided by the invention is simple and convenient to operate and easy to realize.

摘要：【中文】本发明涉及一种高品质食用油自动现榨现售的方法，该方法包括以下步骤：步骤1)，预制标准料；步骤2)，标准料供应到各个压榨销售现场；步骤3)，压榨销售现场按需自动压榨、过滤、暂存和定量灌装销售成品油。本发明能实现在售卖现场自动连续或间断榨油、炼油，暂存、自助灌装和销售食用油，而且食用油品质安全、营养丰富、色泽鲜亮和香味浓郁。 【EN】The invention relates to a method for automatically squeezing and selling high-quality edible oil, which comprises the following steps: step 1), prefabricating a standard material; step 2), supplying standard materials to each squeezing sale site; and 3), automatically squeezing, filtering, temporarily storing and quantitatively filling and selling the finished oil on a squeezing and selling site as required. The invention can realize automatic continuous or discontinuous oil extraction and refining, temporary storage, self-service filling and selling of the edible oil on the selling site, and the edible oil has safe quality, rich nutrition, bright color and strong fragrance.

摘要：【中文】本发明属于食品加工技术领域，尤其涉及一种富含Canolol冷榨菜籽油的制备方法。本发明提供的制备方法包括以下步骤：将油菜籽在气流膨化机中进行加热炒籽，将所得物料进行冷榨，得到冷榨菜籽油。本发明采用气流膨化技术制备冷榨菜籽油，油菜籽中的芥子酸及其衍生物在气流膨化机的密闭空间中加热时，油菜籽水分的汽化和容器内气体的膨胀会产生高压及高温条件，能够使Canolol的产生效率显著提高，进而使得到的菜籽油中Canolol含量高达2156.00mg/kg。 【EN】The invention belongs to the technical field of food processing, and particularly relates to a preparation method of cold-pressed rapeseed oil rich in Canolol. The preparation method provided by the invention comprises the following steps: heating and frying the rapeseeds in an airflow bulking machine, and cold-pressing the obtained material to obtain the cold-pressed rapeseed oil. According to the invention, the cold-pressed rapeseed oil is prepared by adopting an airflow puffing technology, when erucic acid and derivatives thereof in the rapeseed are heated in a closed space of an airflow puffing machine, high-pressure and high-temperature conditions can be generated by the vaporization of moisture of the rapeseed and the expansion of gas in a container, the generation efficiency of Canool can be obviously improved, and the content of Canool in the obtained rapeseed oil can reach 2156.00 mg/kg.