摘要：【中文】本发明公开了一种猪圆环病毒2型重组Cap蛋白的表达制备及应用。猪圆环病毒2型重组Cap蛋白，其序列为序列表中序列2。其编码基因为序列表中序列1。猪圆环病毒2型Cap重组蛋白病毒粒子的制备方法，包括下述步骤：1)表达猪圆环病毒2型Cap重组蛋白重组载体转化大肠杆菌BL21(DE3)感受态，得到表达猪圆环病毒2型Cap重组蛋白病毒粒子的重组菌；2)重组菌诱导表达，收集菌体并破碎，收集包涵体；3)包涵体溶解复性获得组装的猪圆环病毒2型Cap重组蛋白病毒粒子。本发明采用大肠杆菌表达系统表达猪圆环病毒2型Cap蛋白，蛋白表达量大，纯化方法简单，极大降低了生产成本，有利于推进之后的大批量生产。 【EN】The invention discloses an expression preparation and application of porcine circovirus type 2 recombinant Cap protein. The sequence of the porcine circovirus type 2 recombinant Cap protein is the sequence 2 in the sequence table. The coding gene is sequence 1 in the sequence table. The preparation method of the porcine circovirus type 2Cap recombinant protein virion comprises the following steps: 1) the recombinant vector for expressing the porcine circovirus type 2Cap recombinant protein is used for transforming the competence of escherichia coli BL21(DE3) to obtain recombinant bacteria for expressing porcine circovirus type 2Cap recombinant protein virions; 2) inducing expression of the recombinant bacteria, collecting and crushing the bacteria, and collecting inclusion bodies; 3) and (4) carrying out dissolution and renaturation on the inclusion body to obtain the assembled porcine circovirus type 2Cap recombinant protein virion. The invention adopts an escherichia coli expression system to express the porcine circovirus type 2Cap protein, has large protein expression quantity and simple purification method, greatly reduces the production cost and is beneficial to promoting mass production.

摘要：【中文】本发明提供一种抗菌复合酶制剂及其应用。所述复合酶制剂包括葡萄糖氧化酶、β‑甘露聚糖酶和β‑葡聚糖酶。本发明提供的复合酶制剂对大肠杆菌、沙门氏菌、金黄色葡萄球菌和产气荚膜梭菌均具有良好的抑制效果，可作为饲料添加剂，同时可以促进动物的生长，绿色环保，生产方法简便，可替代抗生素使用。制备复合酶制剂所用的原料安全、绿色，具有良好的抑菌效果，能够显著提升畜禽的生产性能，具有较好的市场前景。 【EN】The complex enzyme preparation provided by the invention has good inhibition effects on escherichia coli, salmonella, staphylococcus aureus and clostridium perfringens, can be used as a feed additive, can promote the growth of animals, is green and environment-friendly, has a simple and convenient production method, can replace antibiotics, is safe and green in raw materials for preparing the complex enzyme preparation, has a good antibacterial effect, can remarkably improve the production performance of livestock and poultry, and has a good market prospect.

摘要：【中文】本发明涉及血清的制备方法，具体涉及一种猪乙型脑炎病毒特异性阳性血清及其制备方法。所述制备方法以豚鼠或兔作为免疫动物，采用传代细胞的病毒液作为猪乙型脑炎病毒抗原进行基础免疫。所制得的乙型脑炎特异性阳性血清效价高达1：2000倍以上，比直接免疫猪所得血清效价高，可满足乙型脑炎诊断技术和疫苗质量控制的要求。同时，免疫动物为兔和豚鼠，降低了成本，减少了用猪制备阳性血清因同源动物而引起的同源其他外源病毒污染的风险。 【EN】The invention relates to a preparation method of serum, in particular to specific positive serum for porcine Japanese encephalitis virus and a preparation method thereof. The preparation method takes guinea pigs or rabbits as immune animals, and adopts virus liquid of passage cells as a Japanese encephalitis virus antigen of pigs for basic immunity. The titer of the prepared encephalitis B specific positive serum is as high as 1: more than 2000 times, has higher serum titer than that of the directly immunized pig, and can meet the requirements of Japanese encephalitis diagnosis technology and vaccine quality control. Meanwhile, the immune animals are rabbits and guinea pigs, so that the cost is reduced, and the risk of homologous other exogenous virus pollution caused by homologous animals when the positive serum is prepared by pigs is reduced.

摘要：【中文】本发明涉及免疫学技术领域，具体涉及一种改进的氢氧化铝佐剂及其制备方法。通过运用二氧化碳对氢氧化铝佐剂进行处理，可改善其免疫增强效果，改进后的氢氧化铝佐剂免疫效果明显，佐剂安全有效。同时，本方法工艺过程可控，易于疫苗的工业化生产，为疫苗的开发和应用提供了新的选择。 【EN】The invention relates to the technical field of immunology, in particular to an improved aluminum hydroxide adjuvant and a preparation method thereof. The aluminum hydroxide adjuvant is treated by using carbon dioxide, so that the immune enhancement effect of the aluminum hydroxide adjuvant can be improved, the improved aluminum hydroxide adjuvant has an obvious immune effect, and the adjuvant is safe and effective. Meanwhile, the method has controllable process, is easy for the industrialized production of the vaccine, and provides a new choice for the development and application of the vaccine.

摘要：【中文】本发明公开了一种杆状病毒表达猪瘟E2重组亚单位疫苗的制备方法与应用。所述猪瘟E2重组亚单位疫苗包含猪瘟病毒重组E2蛋白以及药学上可接受的佐剂。所述猪瘟病毒重组E2蛋白，其氨基酸序列为自序列表中序列3的氨基端第1‑339位所示的氨基酸序列、序列表中序列3所示的氨基酸序列、自序列表中序列4的氨基端第1‑339位所示的氨基酸序列、或者序列表中序列4所示的氨基酸序列。本发明提供的猪瘟E2重组亚单位疫苗免疫猪后可诱导产生特异性抗体，具有免疫原性强、安全性好且与当前流行毒株相匹配的优点；同时能够通过鉴别诊断区分疫苗免疫和野毒感染动物，可用于猪瘟病毒的净化。 【EN】The invention discloses a preparation method and application of a baculovirus expression classical swine fever E2 recombinant subunit vaccine. The classical swine fever E2 recombinant subunit vaccine comprises classical swine fever virus recombinant E2 protein and a pharmaceutically acceptable adjuvant. The amino acid sequence of the classical swine fever virus recombinant E2 protein is an amino acid sequence shown in 1 st to 339 th sites of an amino terminal of a sequence 3 in a sequence table, an amino acid sequence shown in the sequence 3 in the sequence table, an amino acid sequence shown in 1 st to 339 th sites of an amino terminal of a sequence 4 in the sequence table, or an amino acid sequence shown in the sequence 4 in the sequence table. After being used for immunizing pigs, the swine fever E2 recombinant subunit vaccine provided by the invention can be induced to generate specific antibodies, and has the advantages of strong immunogenicity, good safety and matching with the current epidemic strains; meanwhile, the vaccine immunity and wild virus infected animals can be distinguished through differential diagnosis, and the method can be used for purifying the classical swine fever virus.