摘要：【中文】本发明涉及鱼类人工繁殖技术领域，具体地说是一种提高长薄鳅人工繁殖效率的方法。具体步骤如下，首先对亲鱼进行精心筛选后暂养在聚氯乙烯网箱中，然后对亲鱼进行分类，并针对不同的发育程度，选择不同的催产针距时间和药物计量，有利于亲鱼的保护和卵母细胞的同步成熟，催产出的卵细胞中未成熟卵粒的比例从10％下降到3％，然后进行催产处理，使精卵混合均匀，最后对亲鱼进行护理，最大限度减少产卵亲鱼的受伤和产后亲鱼的死亡，保护了有限的长薄鳅资源，传统方法产后亲鱼的死亡率高达70％～100％，本技术条件下死亡率为10％。 【EN】The invention relates to the technical field of artificial fish breeding, in particular to a method for improving the artificial breeding efficiency of Leptobotia elongata. The method comprises the following specific steps of firstly carefully screening parent fishes, temporarily breeding the parent fishes in a polyvinyl chloride net cage, classifying the parent fishes, selecting different induced spawning needle pitch time and drug metering according to different development degrees, facilitating the protection of the parent fishes and the synchronous maturation of oocytes, reducing the proportion of immature oocytes in the induced spawning oocytes from 10% to 3%, then carrying out induced spawning treatment to uniformly mix sperms and eggs, and finally nursing the parent fishes, so that the injury of the spawning parent fishes and the death of the postpartum parent fishes are reduced to the maximum extent, limited loach resources are protected, the mortality rate of the postpartum parent fishes in the traditional method is 70% -100%, and the mortality rate under the technical condition is 10%.

摘要：【中文】本发明公开了一种鉴定沙塘鳢种属的引物及方法，该引物的上游引物具有如SEQ.ID NO 6所示的核苷酸序列，其下游引物具有如SEQ.ID NO7所示的核苷酸序列。本发明的引物基于7种沙塘鳢线粒体12s rRNA序列设计，能够实现一对引物同时鉴定7种沙塘鳢，方法简便，从分子水平对中国沙塘鳢属鱼类的种属进行鉴定，具有客观性，更加科学。 【EN】The invention discloses a primer and a method for identifying odontobutis obscura species, wherein an upstream primer of the primer has a nucleotide sequence shown as SEQ ID NO6, and a downstream primer of the primer has a nucleotide sequence shown as SEQ ID NO 7. The primer is designed based on 12s rRNA sequences of 7 odontobutis obscura mitochondria, a pair of primers can simultaneously identify 7 odontobutis obscura, the method is simple and convenient, the species of the Chinese odontobutis obscura fishes can be identified from the molecular level, and the method has objectivity and is more scientific.

摘要：【中文】本发明公开了一种鉴定达氏鲟和中华鲟种质的引物、分子标记及方法，所述的引物核苷酸序列如SEQ ID NO.3～4所示，所述的分子标记为通过所述引物扩增获得的片段，利用本发明引物对基因组DNA进行扩增，对扩增产物进行测序，通过与达氏鲟和中华鲟线粒体DNA序列进行比对，即可鉴定出达氏鲟和中华鲟。本发明方法具有速度快、成本低、易掌握的特点。 【EN】The invention discloses a primer, a molecular marker and a method for identifying germplasms of acipenser dabryanus and acipenser sinensis, wherein the nucleotide sequence of the primer is shown as SEQ ID NO. 3-4, the molecular marker is a fragment obtained by amplification of the primer, the primer is utilized to amplify genome DNA, an amplification product is sequenced, and the amplified product is compared with the mitochondrial DNA sequences of the acipenser dabryanus and the acipenser sinensis, so that the acipenser dabryanus and the acipenser sinensis can be identified. The method has the characteristics of high speed, low cost and easy mastering.

摘要：【中文】本发明公开了一种红螯螯虾的工厂化繁育方法，包括以下步骤：将雌雄红螯螯虾亲虾，放养于繁殖池中；红螯螯虾受精卵颜色变化顺序为豆黄色、深褐色、橙色、深橙红色，选择受精卵发育到深橙红色的抱卵母虾，将其单独放置，进行分隔操作；经过自然交配，收集抱卵母虾放入孵化池进行孵化；将挑选出的受精卵处特定发育时期的抱卵母虾，每只单独放进透明塑料盒中，并且封闭塑料盒限制抱卵母虾的活动；将已放入孵化盒的抱卵母虾进一步细分，根据其怀卵量、卵发育成熟度条件，在每个标粗池中放入合适密度的抱卵母虾。本发明有效减少了收集虾苗、躲避不及时等因素对成活率的影响。本发明通过人为操作的方式，可减少多余的劳动，提高劳动效率。 【EN】The invention discloses an industrial breeding method of red swamp crayfish, which comprises the following steps: stocking female and male parent crayfishes of the red swamp crayfishes in a breeding pond; the color change sequence of fertilized eggs of the red crayfish is bean yellow, dark brown, orange and dark orange red, and the oosperm of the female lobster with eggs which develop to the dark orange red is selected and placed separately for separation operation; collecting the egg-holding shrimps and putting the egg-holding shrimps into an incubation pool for incubation after natural mating; putting each of the selected egg-carrying female shrimps at the fertilized egg position in a specific development period into a transparent plastic box independently, and sealing the plastic box to limit the movement of the egg-carrying female shrimps; and further subdividing the egg-carrying female shrimps which are placed into the hatching boxes, and placing the egg-carrying female shrimps with proper density into each standard coarse pond according to the conditions of the egg-carrying amount and the egg development maturity. The method effectively reduces the influence of factors such as shrimp larvae collection and untimely avoidance on survival rate. The invention can reduce the redundant labor and improve the labor efficiency by a manual operation mode.

摘要：【中文】本发明公开了一种鉴定达氏鲟基因组大小及倍性的方法，包括对达氏鲟总DNA经末端修复、加A尾、加测序接头、纯化、PCR扩增完成整个文库制备，进行进行PE测序，得到的原始数据过滤得到有效数据，采用采用基于K‑mer的分析方法来估计基因组大小，计算杂合率。本发明方法多倍体物种基因组大小及倍性的鉴定提供了一种参考方案。 【EN】The invention discloses a method for identifying the size and ploidy of a Acipenser dabryanus genome, which comprises the steps of carrying out end repair, A tail adding, sequencing joint adding, purification and PCR amplification on the total DNA of the Acipenser dabryanus to complete the preparation of a whole library, carrying out PE sequencing, filtering the obtained original data to obtain effective data, estimating the size of the genome by adopting an analysis method based on a K-mer, and calculating the heterozygosity rate. The method of the invention provides a reference scheme for identifying the size and ploidy of the polyploid species genome.