摘要：【中文】本发明提供了一种干细胞海绵支架及制备方法和应用，涉及生物材料技术领域，本发明提供的干细胞海绵支架，包括纳米纤维海绵及负载于所述纳米纤维海绵上的干细胞，该干细胞海绵支架具有良好的压缩回弹性能，可以大量吸收伤口渗出液，保持伤口湿润，起到防护、止血、促进伤口愈合的作用。并且，三维纤维结构具有良好的网孔连通性、更大的内表面积和孔径，可以提高干细胞的负载数量。此外，本发明提供的干细胞海绵支架具有良好的生物活性、生物降解性和生物相容性，不引起炎症反应或过敏反应。 【EN】The stem cell sponge scaffold provided by the invention comprises nanofiber sponge and stem cells loaded on the nanofiber sponge, has good compression resilience, can absorb a large amount of wound exudates, keeps a wound moist, and plays roles in protecting, stopping bleeding and promoting wound healing. And the three-dimensional fiber structure has good mesh connectivity, larger inner surface area and pore size, and can improve the load quantity of stem cells. In addition, the stem cell sponge scaffold provided by the invention has good biological activity, biodegradability and biocompatibility, and does not cause inflammatory reaction or anaphylactic reaction.

摘要：【中文】本发明属于干细胞芯片技术领域，具体涉及一种基于口腔干细胞的芯片的制备方法。该制备方法包括如下步骤：S1细胞爬片的制备分别将6种口腔干细胞的单细胞悬液接种于细胞基片的各分区框内培养至细胞贴在细胞基片上，随后加入培养基覆盖；取出细胞爬片，用冲洗液冲洗后固定、重溶得6种口腔干细胞细胞悬液；S2细胞芯片的制备将6种口腔干细胞细胞悬液与点样液混合后加入到标准细胞培养板中进行点样后培养，即可制成；其中，所述6种口腔干细胞为DPSCs，SHED，SCAP，PDLSCs，DFSCs和GMSCs。本发明提供的制备方法制得的细胞芯片具有稳定性好、可重复性好、检测准确性高的优点。 【EN】The invention belongs to the technical field of stem cell chips, and particularly relates to a preparation method of a chip based on oral stem cells. The preparation method comprises the following steps: s1 preparation of cell slide, inoculating single cell suspension of 6 kinds of oral stem cells in each partition frame of cell substrate, culturing until the cells are attached to the cell substrate, and adding culture medium to cover; taking out the cell slide, washing with washing liquid, fixing, and re-dissolving to obtain 6 kinds of oral stem cell suspension; s2 preparation of cell chip mix 6 kinds of buccal cavity stem cell suspension with sample solution, add into standard cell culture plate to carry on the culture after the sample solution, can make; wherein, the 6 kinds of oral stem cells are DPSCs, SHED, SCAP, PDLSCs, DFSCs and GMSCs. The cell chip prepared by the preparation method provided by the invention has the advantages of good stability, good repeatability and high detection accuracy.

摘要：【中文】本发明提供了一种青蒿素在制备培养干细胞的产品中的应用、培养基及培养方法，涉及干细胞培养技术领域，本发明通过实验发现，在培养干细胞的过程中通过添加适量的青蒿素，能够有效诱导干细胞的成骨分化能力，并降低诱导过程中诱导物对细胞产生的毒性，保持细胞活性和增殖率。因此，可以利用青蒿素制备培养干细胞的产品。同时，青蒿素为中药单体，成分简单明确，还能够有效避免中药方剂成分复杂、有效物质难以定量、药效作用机理不清、难以推广等问题。 【EN】The invention provides an application of artemisinin in preparation of a product for culturing stem cells, a culture medium and a culture method, and relates to the technical field of stem cell culture. Therefore, artemisinin can be used to prepare a product for culturing stem cells. Meanwhile, the artemisinin is a Chinese medicinal monomer, has simple and definite components, and can effectively solve the problems of complex components, difficult quantification of effective substances, unclear mechanism of action of the medicinal effect, difficult popularization and the like of a Chinese medicinal prescription.