摘要：【中文】本发明提供了一种表达Sox4基因重组腺病毒的制备及纯化方法，属于基因工程领域。该方法将Sox4基因片段与腺病毒pAdtrack‑CMV载体连接，得到重组腺病毒表达质粒pAdtrack‑Sox4。线性化的pAdtrack‑Sox4与AdEasy载体形成的重组子转染HEK293细胞生成腺病毒。用氯化铯梯度离心纯化病毒，并采用微量全细胞病变法检测病毒滴度，最终实现腺病毒包装、生产和纯化的整个过程。该Sox4基因重组腺病毒可成功侵染293T细胞和小鼠腹股沟脂肪组织，实现了Sox4基因在体细胞和小鼠组织中的持续高表达，这为日后开展有关Sox4基因功能的研究奠定了良好的技术基础。 【EN】The invention provides a preparation and purification method of recombinant adenovirus expressing Sox4 gene, belonging to the field of genetic engineering. According to the method, a Sox4 gene fragment is connected with an adenovirus pAdtrack-CMV vector to obtain a recombinant adenovirus expression plasmid pAdtrack-Sox 4. The linear pAdtrack-Sox4 and the AdEasy vector form a recombinant which is used for transfecting HEK293 cells to generate adenovirus. And (3) purifying the virus by cesium chloride gradient centrifugation, and detecting the virus titer by adopting a trace whole-cell lesion method, thereby finally realizing the whole process of adenovirus packaging, production and purification. The recombinant adenovirus of the Sox4 gene can successfully infect 293T cells and mouse inguinal adipose tissues, realizes the continuous high expression of the Sox4 gene in somatic cells and mouse tissues, and lays a good technical foundation for the research on the functions of the Sox4 gene in the future.

摘要：【中文】本发明涉及一种环基坑水平砂井降水疏干结构及降水疏干方法，其环基坑水平砂井降水疏干结构包括若干个环绕基坑间隔设置的竖向砂井和至少一个环绕基坑设置的水平砂井，水平砂井位于基坑所在的含水层中，水平砂井与竖向砂井相连，水平砂井还连接有若干个竖向管井，竖向管井包括滤水段和储水段，滤水段和储水段的分界线位于最下方水平砂井的底界面上或低于最下方水平砂井的底界面，储水段内设置有抽水设备。本发明在达到对基坑降水疏干目的时，采用的水平砂井结构底界面的位置很浅，其远小于垂直井点深度，从而避免抽取更多无关地下水，使因多抽取地下水、多抽取深层地下水而引发的一系列问题得到缓解，甚至得到较好的解决。 【EN】The invention relates to a dewatering and draining structure and a dewatering and draining method for a horizontal sand well of an annular foundation pit. When the invention achieves the aim of dewatering and draining the foundation pit, the position of the bottom interface of the adopted horizontal sand well structure is shallow and is far smaller than the depth of the vertical well point, thereby avoiding pumping more irrelevant underground water, relieving a series of problems caused by pumping more underground water and pumping more deep underground water, and even better solving the problems.

摘要：【中文】本发明涉及一种水下土体扩搅器，所述水下土体扩搅器固定设置在钻杆下端，所述钻杆为带芯管的双重管钻杆，所述水下土体扩搅器包括外管、内管、切削搅拌架、钻头和输浆系统，所述外管上端与钻杆固定连接，所述内管上下滑动套合在外管内下部，所述切削搅拌架圆周阵列设置在外管和内管之间，所述钻头固定设置在内管下端；一种水下扩底桩的施工方法，包括以下步骤：确定扩搅范围，确定水下土体扩搅器的尺寸，选择钻杆，配制浆液，钻直孔，进行扩搅，取土，浇灌扩底桩。本发明的目的是为了解决水下扩底时周围的无粘性土层不能自稳、容易坍塌的问题，提供一种水下土体扩搅器及水下扩底桩的施工方法。 【EN】The invention relates to an underwater soil body spreading and stirring device which is fixedly arranged at the lower end of a drill rod, wherein the drill rod is a dual-pipe drill rod with a core pipe, the underwater soil body spreading and stirring device comprises an outer pipe, an inner pipe, a cutting and stirring frame, a drill bit and a slurry conveying system, the upper end of the outer pipe is fixedly connected with the drill rod, the inner pipe is sleeved at the lower part in the outer pipe in an up-and-down sliding manner, the cutting and stirring frame is circumferentially arranged between the outer pipe and the inner pipe in an array manner, and the drill bit is fixedly arranged at the lower end of the inner; a construction method of an underwater club-footed pile comprises the following steps: determining the expansion and stirring range, determining the size of the underwater soil body expansion and stirring device, selecting a drill rod, preparing slurry, drilling a straight hole, performing expansion and stirring, taking soil and irrigating a pedestal pile. The invention aims to solve the problems that surrounding cohesionless soil layers cannot be self-stabilized and are easy to collapse during underwater bottom expanding, and provides an underwater soil body expanding stirrer and a construction method of an underwater bottom expanding pile.

摘要：【中文】本发明公开了一种基于变换器的串联电池组直接均衡方法，该直接均衡方法所基于的均衡电路包括串联电池组、2n+3个MOS管、2n+5个二极管、两个反激变换器、一个RCD缓冲电路；串联电池组由n个单体电池组成。本发明特点在于：①整个均衡电路只需要两个反激变换器用于能量转移，体积较小。②易于扩展，当串联电池组包含的单体电池数量发生改变时，只需要增加相应的MOS管；③将串联电池组充放电过程的均衡控制策略合二为一，充放电过程中，同时实现对最大端电压的单体电池放电均衡和最小端电压的单体电池充电均衡。 【EN】The invention discloses a direct equalization method of a series battery pack based on a converter, wherein an equalization circuit based on the direct equalization method comprises the series battery pack, 2n +3 MOS (metal oxide semiconductor) tubes, 2n +5 diodes, two flyback converters and an RCD (remote control device) buffer circuit, wherein the series battery pack consists of n single batteries.

摘要：【中文】本发明涉及一种扩孔空间内粗砾芯墙填充装置，包括中导管、上边导管、多个接管、管瓣座、支撑座和活动支架；所述中导管内设置有套管，中导管两侧分布上边导管，中导管和上边导管均固定设置在管瓣座上部，多个所述接管竖直向排布在中导管上部；所述支撑座下部设置有锥形底座、上部设置有导杆，所述导杆上部置于套管内滑动，导杆和套管之间设置有锁止机构；所述管瓣座和支撑座之间设置所述活动支架。一种基于扩孔空间内粗砾芯墙填充装置的充填方法，包括以下步骤：将本装置以收缩状态放入孔底；通过锁止机构将本装置以展开状态固定；向孔内投放石料，并多次提升本装置，以构筑芯墙。本发明设计合理，实现了对竖向砂井扩孔空间内粗砾芯墙的制造。 【EN】The invention relates to a gravel core wall filling device in a reaming space, which comprises a middle guide pipe, an upper guide pipe, a plurality of connecting pipes, a pipe valve seat, a supporting seat and a movable support, wherein the middle guide pipe is connected with the upper guide pipe; the middle guide pipe is internally provided with a sleeve pipe, the two sides of the middle guide pipe are distributed with upper guide pipes, the middle guide pipe and the upper guide pipes are fixedly arranged at the upper part of the valve seat, and the connecting pipes are vertically arranged at the upper part of the middle guide pipe; the lower part of the supporting seat is provided with a conical base, the upper part of the supporting seat is provided with a guide rod, the upper part of the guide rod is arranged in the sleeve to slide, and a locking mechanism is arranged between the guide rod and the sleeve; the movable support is arranged between the tube valve seat and the supporting seat. A filling method based on a coarse gravel core wall filling device in a reaming space comprises the following steps: putting the device into the bottom of the hole in a contracted state; the device is fixed in the unfolding state through a locking mechanism; and (5) putting stones into the holes, and lifting the device for multiple times to construct the core wall. The invention has reasonable design and realizes the manufacture of the coarse gravel core wall in the reaming space of the vertical sand well.

摘要：【中文】本发明涉及一种用于维护共享电车的立体存取装置及方法,包括车架和充电桩，车架和充电桩的外侧设置有车库，车库为一端敞口、内部中空的方形结构，车库的顶部设置有用于吊取共享电车的存放机构，存放机构包括吊爪、卷扬机以及丝杆，卷扬机的钢丝绳与吊爪固定连接、卷扬机的外壳与丝杆固定连接，丝杆设置有步进电机；存放机构连接有用于自动吊取共享电车的控制系统，控制系统包括检测单元和控制单元，检测单元用于发出吊取信号，控制单元用于控制存放机构完成吊取动作；车架呈方形等距阵列，车架将车库分为多层。本发明设置有车库、存放机构和控制系统，在存放区域内共享电车可实现自动存车、取车以及充电。 【EN】The invention relates to a three-dimensional storing and taking device and a three-dimensional storing and taking method for maintaining a shared electric car, and the three-dimensional storing and taking device comprises a frame and a charging pile, wherein a garage is arranged on the outer sides of the frame and the charging pile, the garage is of a square structure with an open end and a hollow interior, a storage mechanism for lifting and taking the shared electric car is arranged at the top of the garage, the storage mechanism comprises a lifting claw, a winch and a screw rod, a steel wire rope of the winch is fixedly connected with the lifting claw, a shell of the winch is fixedly connected with the screw rod; the storage mechanism is connected with a control system for automatically hoisting the shared electric car, the control system comprises a detection unit and a control unit, the detection unit is used for sending a hoisting signal, and the control unit is used for controlling the storage mechanism to complete hoisting action; the vehicle frames are in square equidistant array, and the vehicle frames divide the garage into a plurality of layers. The invention is provided with a garage, a storage mechanism and a control system, and can realize automatic parking, car taking and charging by sharing the electric car in the storage area.

摘要：【中文】本发明公开了一种基于目标感知相关滤波的目标跟踪方法，其包括以下步骤：步骤1、构建深度网络特征提取模型；步骤2、确定跟踪目标及标签；步骤3、提取目标深度特征，构建通道选择相关滤波器；步骤4、滤波器逆变换，取实部并全局池化求绝对值，选择L个较大值对应的深度特征构建目标感知相关滤波器模型；步骤5、确定目标搜索区域，提取L个通道特征；步骤6、应用目标感知相关滤波器确定跟踪目标位置及尺度；步骤7、应用线性迭代更新通道选择相关滤波器；步骤8、应用线性迭代更新目标感知相关滤波器；步骤9、循环执行，实现目标连续跟踪。本发明可实现跟踪目标的自动感知，通道权重的在线自适应更新，有效增强跟踪算法的鲁棒性。 【EN】The invention discloses a target tracking method based on target perception correlation filtering, which comprises the following steps: step 1, constructing a deep network feature extraction model; step 2, determining a tracking target and a label; step 3, extracting target depth features and constructing a channel selection correlation filter; step 4, inverse transformation of the filter, namely taking a real part and solving an absolute value through global pooling, and selecting depth features corresponding to L larger values to construct a target perception correlation filter model; step 5, determining a target search area and extracting L channel characteristics; step 6, determining the position and the scale of a tracked target by applying a target perception correlation filter; step 7, updating a channel selection correlation filter by applying linear iteration; step 8, updating the target perception correlation filter by applying linear iteration; and 9, circularly executing to realize continuous target tracking. The invention can realize the automatic perception of the tracking target and the online self-adaptive updating of the channel weight, and effectively enhance the robustness of the tracking algorithm.

摘要：【中文】本发明公开了一种基于双层深度特征感知的目标跟踪方法，其包括以下步骤：步骤1、确定跟踪目标及Padding窗；步骤2、提取目标区域深度特征并加窗处理，进行第一层特征感知；步骤3、依据第一层感知特征创建特征感知相关滤波器；步骤4、计算特征感知相关滤波器的梯度图均值，选择L个较大值通道特征，完成第二层特征感知；步骤5、依据上一帧目标位置确定搜索区域尺度图像集合，提取第二层感知特征；步骤6、应用相关滤波确定目标位置及尺度；步骤7、应用跟踪结果的第一层感知特征更新特征感知相关滤波器；步骤8、循环执行步骤4、5、6、7。本发明可以解决基于像素点误差的目标感知效率较低的问题。 【EN】The invention discloses a target tracking method based on double-layer depth feature perception, which comprises the following steps: step 1, determining a tracking target and a Padding window; step 2, extracting depth features of the target area, performing windowing processing, and performing first-layer feature perception; step 3, establishing a feature perception correlation filter according to the first layer perception feature; step 4, calculating the average value of the gradient image of the feature perception correlation filter, selecting L channel features with larger values, and finishing second-layer feature perception; step 5, determining a search area scale image set according to the position of the previous frame of target, and extracting second-layer perception features; step 6, determining the position and the scale of the target by applying relevant filtering; step 7, updating the feature perception correlation filter by applying the first layer perception feature of the tracking result; and 8, circularly executing the steps 4, 5, 6 and 7. The invention can solve the problem of low target perception efficiency based on pixel point errors.

摘要：【中文】本发明公开了一种可测量基质吸力的非饱和土湿陷真三轴仪，包括固定在主机底座上面的主机支架，主机底座上面安装有轴向调节活塞，轴向调节活塞的上面安装有轴向液压缸，轴向液压缸内设置有轴向压力活塞，轴向压力活塞的上面固定有压力室，压力室内部开设有试样腔，压力室底座的内部设置有分别与试样腔连通的下进水管和下排水管，试样帽上设置有上浸水管和上进气管，上浸水管连接有GDS压力/体积控制器，压力室侧壁内设置有四个空腔，每个空腔内都设置有柔性液压囊，每个柔性液压囊通过侧向压力开关阀与步进电机驱动控制器连接。本发明结构更惊细，易于操作，可实现控制吸力和测量吸力条件下的非饱和土真三轴湿陷试验。 【EN】The invention discloses an unsaturated soil collapsibility true triaxial apparatus capable of measuring substrate suction, which comprises a host bracket fixed on a host base, wherein an axial adjusting piston is installed on the host base, an axial hydraulic cylinder is installed on the axial adjusting piston, an axial pressure piston is arranged in the axial hydraulic cylinder, a pressure chamber is fixed on the axial pressure piston, a sample chamber is arranged in the pressure chamber, a lower water inlet pipe and a lower water outlet pipe which are respectively communicated with the sample chamber are arranged in the pressure chamber base, an upper water immersion pipe and an upper air inlet pipe are arranged on a sample cap, the upper water immersion pipe is connected with a GDS pressure/volume controller, four cavities are arranged in the side wall of the pressure chamber, a flexible hydraulic bag is arranged in each cavity, and each flexible hydraulic bag is connected with a stepping motor driving controller through a lateral pressure switch valve. The invention has more surprisingly fine structure and easy operation, and can realize the unsaturated soil true triaxial collapsible test under the conditions of controlling the suction force and measuring the suction force.

摘要：【中文】本发明公开一种韧性水泥基压电复合材料、制备方法及应用，该复合材料是通过将一定比例的钙钛矿压电晶体和水泥分散到PVDF基压电材料中制得的。该复合材料不仅压电性能优异，且韧性高，与混凝土材料的相容性好，解决了现有水泥基压电材料脆性大，与混凝土结构整体结合性差的问题。且该复合材料应用于土木工程中混凝土建筑结构的传感器的制备，所制备的传感器的灵敏度、传感精度高，降低了安全隐患。 【EN】The invention discloses a tough cement-based piezoelectric composite material, a preparation method and application thereof. The composite material has excellent piezoelectric property, high toughness and good compatibility with concrete materials, and solves the problems of large brittleness and poor integral bonding property with a concrete structure of the existing cement-based piezoelectric material. The composite material is applied to the preparation of the sensor of the concrete building structure in civil engineering, the sensitivity and the sensing precision of the prepared sensor are high, and the potential safety hazard is reduced.