摘要：【中文】本发明公开了一种靶向间充质干细胞外泌体，该靶向间充质干细胞外泌体的配方包括以下组份：间充质干细胞15％‑25％、氨基酸1‑2％、葡萄糖3‑8％、无水氯化钙1‑5％、亮氨酸3‑6％、氯化钾2‑4％、次黄嘌呤1‑2％、胸苷1‑3％、核黄素2‑6％、苯丙氨酸4‑8％、氯化钠0.09‑2％、乳酸钠0.1‑2％、氯化钙0.09‑2％、必须氨基酸0.01‑5％、非必须氨基酸0.01‑5％、人血清白蛋白0.1‑5％，其余为去离子水；本发明还公开了靶向间充质干细胞外泌体的制备方法及其应用，该制备方法包括以下步骤：定量称取，间充质干细胞培养，除菌，囊泡颗粒分离，重悬处理，获取分泌体制剂，注射使用；本发明制备出的靶向间充质干细胞外泌体得到较大提升，在其制备过程中通过除菌级亲水性过滤器对其培养基的上清液杀菌处理，避免其滋生有害细菌。 【EN】The invention discloses a targeting mesenchymal stem cell exosome, which comprises the following components in part by weight: 15-25% of mesenchymal stem cells, 1-2% of amino acid, 3-8% of glucose, 1-5% of anhydrous calcium chloride, 3-6% of leucine, 2-4% of potassium chloride, 1-2% of hypoxanthine, 1-3% of thymidine, 2-6% of riboflavin, 4-8% of phenylalanine, 0.09-2% of sodium chloride, 0.1-2% of sodium lactate, 0.09-2% of calcium chloride, 0.01-5% of essential amino acid, 0.01-5% of non-essential amino acid, 0.1-5% of human serum albumin and the balance of deionized water; the invention also discloses a preparation method and application of the targeted mesenchymal stem cell exosome, wherein the preparation method comprises the following steps: quantitatively weighing, culturing mesenchymal stem cells, sterilizing, separating vesicle particles, carrying out heavy suspension treatment, obtaining a secretion preparation, and injecting for use; the targeted mesenchymal stem cell exosome prepared by the method is greatly improved, and the supernatant of the culture medium is sterilized by a sterilization-grade hydrophilic filter in the preparation process, so that harmful bacteria are prevented from breeding.

摘要：【中文】本申请公开了一种内侧髌骨韧带的解剖止点定位方法，包括以下步骤：采用手法定位方式确定参考止点，在该参考止点处固定金属止点标记；采用手法定位方式确定参考中点，在该参考中点处固定金属中点标记；携带该金属止点标记以及金属中点标记由CT透视获得患膝的第一透视图以及第二透视图；对该第一透视图以及第二透视图进行复合，确定滑车轨迹及其转动中心，设置该转动中心为图像矫正点；基于测量标尺测得该图像矫正点与参考止点图像和参考中点图像的距离和角度数值；根据该距离和角度数值，在患膝体表参照该参考止点标记作为手术入路点的修正止点。借助本申请的方法可为患者提供定制的手术入路，减少术后并发症的发生率以及缩短手术时间。 【EN】The application discloses a method for positioning an anatomical insertion point of a medial patellar ligament, which comprises the following steps: determining a reference dead point by adopting a manual positioning mode, and fixing a metal dead point mark at the reference dead point; determining a reference midpoint by adopting a manual positioning mode, and fixing a metal midpoint mark at the reference midpoint; carrying the metal dead point mark and the metal midpoint mark to obtain a first perspective view and a second perspective view of the affected knee through CT fluoroscopy; compounding the first perspective view and the second perspective view, determining a track of the pulley and a rotation center of the pulley, and setting the rotation center as an image correction point; measuring the distance and angle values between the image correction point and the reference dead point image and the reference midpoint image based on the measuring scale; and according to the distance and angle values, the reference stop mark is referred to as a correction stop of the surgical access point on the surface of the affected knee. With the methods of the present application, customized surgical access can be provided to the patient, reducing the incidence of postoperative complications and shortening the surgical time.

摘要：【中文】本发明公开了一种足部病理筛查架，包括透明玻璃、平面镜和支架，所述透明玻璃位于支架的顶面，所述平面镜位于透明玻璃下方，并且与透明玻璃呈角度设置。本发明通过平面镜透过透明玻璃，投影出用户的足部特征，能够简单方便快捷的观察到足部特征。 【EN】The invention discloses a foot pathology screening frame which comprises transparent glass, a plane mirror and a support, wherein the transparent glass is positioned on the top surface of the support, and the plane mirror is positioned below the transparent glass and forms an angle with the transparent glass. According to the invention, the foot characteristics of the user are projected through the transparent glass by the plane mirror, so that the foot characteristics can be simply, conveniently and rapidly observed.

摘要：【中文】本发明提供了一种羊水比色装置，包括：壳体，在壳体的轴向延伸方向上具有前端及与所述前端相对的尾端，前端设有可视的开口；设置于壳体内部至少两根管状羊水容器，至少两根管状羊水容器间隔设置，形成对比观察通道；设置于管状羊水容器与尾端之间的比色卡安装槽；比色卡组，包含若干能够装入比色卡安装槽并能够被通过对比观察通道观察到的比色卡；比色卡的形成有带有色彩的若干条状涂覆表面，当比色卡装入比色卡安装槽时，条状涂覆表面间隔设置并且与对比观察通道对其或者大致对齐；并且，条状涂覆表面穿过对比观察通道的成像距离可调。以期在设施受限的环境对通过羊水的颜色检测获取信息，并且降低对检测人员的经验要求，并可量化羊水外观的程度。 【EN】The invention provides an amniotic fluid colorimetric device, which comprises: the shell is provided with a front end and a tail end opposite to the front end in the axial extension direction of the shell, and the front end is provided with a visible opening; at least two tubular amniotic fluid containers are arranged in the shell, and the at least two tubular amniotic fluid containers are arranged at intervals to form a contrast observation channel; a colorimetric card mounting groove arranged between the tubular amniotic fluid container and the tail end; the color comparison card group comprises a plurality of color comparison cards which can be arranged in the color comparison card mounting grooves and can be observed through the comparison observation channel; the colorimetric card is provided with a plurality of strip-shaped coating surfaces with colors, and when the colorimetric card is arranged in the colorimetric card mounting groove, the strip-shaped coating surfaces are arranged at intervals and are aligned with or approximately aligned with the contrast observation channel; and the imaging distance of the strip-shaped coating surface through the contrast observation channel is adjustable. So as to acquire information through the color detection of the amniotic fluid in an environment with facility limitation, reduce the experience requirements on detection personnel and quantify the degree of the appearance of the amniotic fluid.

摘要：【中文】本申请涉及一种端粒酶活性检测试剂盒，包括端粒酶底物引物、dNTPs、端粒酶逆转录缓冲液、量子点分子信标，其中量子点分子信标由寡核苷酸茎环、量子点荧光基团和淬灭基团组成，量子点荧光基团与寡核苷酸茎环的序列的5’端连接，淬灭基团与寡核苷酸茎环的序列的3’端连接，其中量子点荧光基团优选为CdTe:Zn²⁺量子点，淬灭基团优选为BHQ2，即量子点分子信标优选为QD‑BHQ2。该量子点分子信标无需过多的化学修饰，无需进行信号放大，具有优异的生物相容性、荧光恢复、检测灵敏度和光稳定性，并且没有细胞毒性。本申请的端粒酶活性检测试剂盒可直接用于端粒酶活性的灵敏可视化检测。 【EN】The application relates to a telomerase activity detection kit, which comprises telomerase substrate primers, dNTPs, telomerase reverse transcription buffer solution and a quantum dot molecular beacon, wherein the quantum dot molecular beacon consists of an oligonucleotide stem ring, a quantum dot fluorescent group and a quenching group, the quantum dot fluorescent group is connected with the 5 'end of the sequence of the oligonucleotide stem ring, the quenching group is connected with the 3' end of the sequence of the oligonucleotide stem ring, and the quantum dot fluorescent group is preferably CdTe: Zn²⁺The quantum dot and the quenching group are preferably BHQ2, namely the quantum dot molecular beacon is preferably QD-BHQ 2. The quantum dot molecular beacon does not need excessive chemical modification, does not need signal amplification, has excellent biocompatibility, fluorescence recovery, detection sensitivity and light stability, and has no cytotoxicity. The telomerase activity detection kit can be directly used for sensitive visual detection of telomerase activity.

摘要：【中文】本申请公开了一种间充质干细胞及其应用。本申请的间充质干细胞是经过抑制Sirt1基因表达处理或Sirt1基因敲除的间充质干细胞。本申请的间充质干细胞，能够促进造血干细胞体外扩增、增强造血干细胞功能、提高移植后造血干细胞重建能力，从而获得功能更好的造血干细胞，为临床解决造血干细胞短缺难题提供了一种新的解决方案，对于解决造血干细胞的临床问具有重要意义。 【EN】The application discloses a mesenchymal stem cell and application thereof. The mesenchymal stem cells of the present application are mesenchymal stem cells that have been treated to inhibit Sirt1 gene expression or have been subjected to Sirt1 gene knockout. The mesenchymal stem cells can promote the in-vitro amplification of the hematopoietic stem cells, enhance the function of the hematopoietic stem cells and improve the reconstruction capacity of the hematopoietic stem cells after transplantation, thereby obtaining the hematopoietic stem cells with better functions, providing a new solution for solving the problem of hematopoietic stem cell shortage clinically and having important significance for solving the clinical problem of the hematopoietic stem cells.

摘要：【中文】本申请公开了全反式维甲酸在间充质干细胞分化调节中的应用。本申请的全反式维甲酸在间充质干细胞分化调节中的应用，具体包括采用全反式维甲酸同时抑制间充质干细胞向成骨细胞和脂肪细胞的分化。本申请经过研究证实全反式维甲酸能够同时抑制间充质干细胞向成骨细胞和脂肪细胞的分化，本申请基于此提供了全反式维甲酸的新的功能用途，这为间充质干细胞的分化调节研究，以及定向调控间充质干细胞分化潜能，提供了一种新的工具和调节手段。 【EN】The application discloses application of all-trans retinoic acid in mesenchymal stem cell differentiation regulation. The application of the all-trans-retinoic acid in the differentiation regulation of the mesenchymal stem cells specifically comprises the step of adopting the all-trans-retinoic acid to simultaneously inhibit the differentiation of the mesenchymal stem cells into osteoblasts and adipocytes. Research proves that the all-trans-retinoic acid can inhibit the differentiation of the mesenchymal stem cells into osteoblasts and adipocytes simultaneously, and the application provides a new functional application of the all-trans-retinoic acid based on the research, so that a new tool and a new regulating means are provided for the research on the differentiation regulation of the mesenchymal stem cells and the directional regulation of the differentiation potential of the mesenchymal stem cells.

摘要：【中文】本发明公开了一种一次性咽拭子采取装置，涉及到医疗器械技术领域，包括套管、固定开口器、面罩、采集管、电源模块和贴片灯珠，套管上设有固定开口器和面罩，面罩位于固定开口器的一侧，采集管套设在套管内，且采集管的一端突出于套管的一端，采集管的另一端突出于套管的另一端，套管的一端的外缘均匀的设有若干贴片灯珠，且若干贴片灯珠位于固定开口器远离面罩的一侧，套管的另一端可拆卸地设有电源模块，电源模块位于面罩远离固定开口器的一侧，每一贴片灯珠分别与电源模块电连接。其具有结构简单、方便使用、能够起到支撑患者下颌关节，防止患者长时间张开口部导致下颌关节受累，同时也能够防止患者唾液传播、安全性高的特点。 【EN】The invention discloses a disposable throat swab taking device, which relates to the technical field of medical instruments and comprises a sleeve, a fixed mouth gag, a mask, a collecting tube, a power module and patch lamp beads, wherein the sleeve is provided with the fixed mouth gag and the mask, the mask is positioned on one side of the fixed mouth gag, the collecting tube is sleeved in the sleeve, one end of the collecting tube protrudes out of one end of the sleeve, the other end of the collecting tube protrudes out of the other end of the sleeve, the outer edge of one end of the sleeve is uniformly provided with a plurality of patch lamp beads, the patch lamp beads are positioned on one side, far away from the mask, of the fixed mouth gag, the other end of the sleeve is detachably provided with the power module, the power module is positioned on one side, far away from the fixed mouth gag. It has simple structure, facilitates the use, can play and support patient's lower jaw joint, prevents that the patient from opening the oral area for a long time and leading to lower jaw joint to be tired, also can prevent that patient's saliva from spreading simultaneously, characteristics that the security is high.