摘要：【中文】本发明提供了一种裂殖壶藻的发酵工艺，该发酵工艺包括如下步骤：(1)取裂殖壶藻藻种，制备种子液；(2)将步骤(1)制备种子液接入培养基中，其中，种子液接种量为2‑3％；(3)在温度为25‑28℃的条件下发酵48‑56h，即可。本发明发酵工艺制备的裂殖壶藻藻粉蛋白含量在45‑50％，脂肪酸含量在25‑35％，DHA含量在10‑15％，相较于现有技术的藻粉更适用于水产养殖。且本发明发酵工艺通过控制碳源、氮源流加时间，可在较短的发酵时间内获得较高的裂殖壶藻生物量和DHA含量。同时，本发明发酵过程大幅降低了原料中氮源成本和总发酵成本。此外，本发明流加碳源、氮源过程易操作，适合裂殖壶藻大规模发酵，有利于工业化生产。 【EN】The invention provides a fermentation process of schizochytrium limacinum, which comprises the following steps: (1) taking schizochytrium limacinum algae seeds, and preparing a seed solution; (2) inoculating the seed solution prepared in the step (1) into a culture medium, wherein the inoculation amount of the seed solution is 2-3%; (3) fermenting at 25-28 deg.C for 48-56 h. The schizochytrium limacinum algae powder prepared by the fermentation process has the protein content of 45-50%, the fatty acid content of 25-35% and the DHA content of 10-15%, and is more suitable for aquaculture compared with the algae powder in the prior art. In addition, the fermentation process can obtain higher schizochytrium biomass and DHA content in shorter fermentation time by controlling the feeding time of the carbon source and the nitrogen source. Meanwhile, the fermentation process greatly reduces the cost of the nitrogen source in the raw materials and the total fermentation cost. In addition, the process of feeding the carbon source and the nitrogen source is easy to operate, is suitable for large-scale fermentation of schizochytrium limacinum, and is beneficial to industrial production.

摘要：【中文】本发明公开了一种循环水系统的鲤鱼投喂方法，根据水流速度影响下的营养代谢规律和循环水系统特点进行喂养，针对鲤鱼对水流作用下的生理规律，水流刺激下使鲤鱼持续运动，流速提升鲤鱼对能量需求逐渐增强，随着时间的延伸，其体内各项代谢酶活动增强，肠道的吸收功能提高，使其饲料转化效率升高，分别配置初期专用饲料和中后期专用饲料，使饲料利用效率提高，达到提高鲤鱼生长速度，保证水质稳定，提高养殖效益的目的。 【EN】The invention discloses a carp feeding method of a circulating water system, which is characterized in that feeding is carried out according to a nutrition metabolism rule and the characteristics of the circulating water system under the influence of a water flow speed, the carp is enabled to continuously move under the stimulation of the water flow according to a physiological rule under the action of the carp on the water flow, the energy demand of the carp is gradually enhanced by increasing the flow speed, various metabolic enzymes in the carp are enhanced along with the extension of time, the absorption function of intestinal tracts is improved, the feed conversion efficiency is improved, and the aims of improving the growth speed of the carp, ensuring stable water quality and improving the culture benefit are fulfilled by respectively configuring an initial-stage special feed and a middle-and-later-stage special feed.

摘要：【中文】本发明公开了一种鱼肉中土腥味物质的测定方法，该测定方法是以顺式‑十氢‑1‑萘酚溶液为内标制备的鱼肉为样品，采用顶空固相微萃取法提取样品中的土臭味素和2‑甲基异莰醇，再通过气相色谱质谱联用法进行的测定过程。采用顶空固相微萃取法提取鱼肉中的土腥味物质，以顺式‑十氢‑1‑萘酚为内标，气相色谱质谱联用法进行定性和定量测定，旨在建立鱼肉中土臭味素和2‑甲基异莰醇的检测方法，为养殖水产品异味物质脱除提供检测方法依据。 【EN】The invention discloses a method for measuring earthy substances in fish, which takes the fish prepared by taking cis-decahydro-1-naphthol solution as an internal standard as a sample, adopts a headspace solid phase microextraction method to extract earthy odorsubstance and 2-methylisoborneol in the sample, and then adopts a gas chromatography-mass spectrometry combined measuring process. A headspace solid phase microextraction method is adopted to extract earthy flavor substances in fish, cis-decahydro-1-naphthol is taken as an internal standard, and a gas chromatography-mass spectrometry combined method is used for qualitative and quantitative determination, so that the method aims to establish a method for detecting the earthy flavor substances and 2-methylisoborneol in the fish and provide a detection method basis for removing the odorous substances in the cultured aquatic products.