摘要：【中文】本发明提供一种微球试剂的制备方法，所述方法包括如下步骤：1)将微球溶液、血清淀粉样蛋白A抗体溶液和偶联剂溶液混合、超声分散；2)向步骤1)分散后的溶液加入第三助剂，混合、超声分散；3)向步骤2)分散后的溶液加入第四助剂，混合，得到所述微球试剂。本发明采用一步法制备所述微球试剂，只需将微球溶液、血清淀粉样蛋白A抗体溶液和偶联剂溶液按照特定比例混合，再利用超声分散，同时再配合各步骤条件，各个因素协同增效，得到了分散均匀稳定的微球试剂体系。该方法操作简单，受设备的限制小，单次生产量大，有效提高了生产效率，降低了生产成本。 【EN】The invention provides a preparation method of a microsphere reagent, which comprises the following steps: 1) mixing the microsphere solution, the serum amyloid A antibody solution and the coupling agent solution, and performing ultrasonic dispersion; 2) adding a third auxiliary agent into the solution dispersed in the step 1), mixing and ultrasonically dispersing; 3) adding a fourth auxiliary agent into the solution dispersed in the step 2), and mixing to obtain the microsphere reagent. The invention adopts a one-step method to prepare the microsphere reagent, and only the microsphere solution, the serum amyloid A antibody solution and the coupling agent solution are mixed according to a specific proportion, and then ultrasonic dispersion is utilized, and simultaneously the conditions of all the steps are matched, and all the factors are synergized, so that a microsphere reagent system with uniform and stable dispersion is obtained. The method has the advantages of simple operation, small limitation of equipment, large single-pass production, effective improvement of production efficiency and reduction of production cost.

摘要：【中文】本发明涉及一种生物素偶联抗体及其制备方法和应用。所述制备方法包括通过酶切还原抗体以获得有固定反应位点的抗体，再将其与生物素进行偶联，获得生物素偶联抗体。本发明所涉及的获得生物素化抗体的方法克服了现有技术中通过修饰后的生物素直接与抗体连接的弊端，即能使抗体获得固定的精确的反应位点，从而控制每个抗体上连接的生物素的精确含量，最终使得酶促化学发光反应中的本底值降低。 【EN】The invention relates to a biotin coupling antibody and a preparation method and application thereof. The preparation method comprises the steps of reducing the antibody through enzyme digestion to obtain the antibody with a fixed reaction site, and coupling the antibody with biotin to obtain a biotin-coupled antibody. The method for obtaining the biotinylated antibody overcomes the defect that the modified biotin is directly connected with the antibody in the prior art, namely, the antibody can obtain fixed accurate reaction sites, so that the accurate content of the biotin connected on each antibody is controlled, and the background value in the enzymatic chemiluminescence reaction is finally reduced.