摘要：【中文】本发明公开了一种基于光纤传输型荧光寿命引导的穿刺活检方法及装置，包括(a)光纤传输型荧光寿命探测装置将脉冲激光经过激发光纤传输后照射在待测活体组织上；(b)从待测活体组织激发出来的荧光信号由探测装置的收集光纤接收，进入荧光探测模块，得到荧光寿命信息和荧光光谱信息；(c)鉴别是否为病变组织；(d)移动探测装置探测位置，重复步骤a到c，检测病变组织所在的位置；(e)进行穿刺活检，选取合适的穿刺点，调整好活检装置的位置、角度和方向，然后触发活检装置穿刺，取出病变组织。本发明对活体组织微弱的自发荧光光谱及荧光寿命进行高灵敏度的检测，能够从细胞病理生理等微观层面区别病变组织及正常组织，继而触发穿刺活检取到病变组织。 【EN】The invention discloses a puncture biopsy method and a device based on optical fiber transmission type fluorescence life guidance, comprising (a) an optical fiber transmission type fluorescence life detection device irradiates pulse laser on a living tissue to be detected after the pulse laser is transmitted by an excitation optical fiber; (b) a fluorescence signal excited from the living tissue to be detected is received by a collecting optical fiber of the detection device and enters a fluorescence detection module to obtain fluorescence life information and fluorescence spectrum information; (c) identifying whether the tissue is pathological or not; (d) moving the detection device to detect the position, repeating the steps a to c, and detecting the position of the lesion tissue; (e) and performing puncture biopsy, selecting a proper puncture point, adjusting the position, the angle and the direction of the biopsy device, triggering the biopsy device to puncture, and taking out the pathological tissue. The invention carries out high-sensitivity detection on the weak autofluorescence spectrum and the fluorescence life of the living tissue, can distinguish the pathological change tissue and the normal tissue from the microscopic level of cell pathophysiology and the like, and then triggers the puncture biopsy to obtain the pathological change tissue.