摘要：【中文】针筒式一次性穴位贴制作仪，包括针筒、拆分式推杆和针筒盖，拆分式推杆滑动安装在针筒内，拆分式推杆可以用于对中药进行搅拌，也可以用于将中药推出针筒外，针筒盖可拆式安装在针筒的端部，在针筒盖内设有通孔，拆分式推杆通过通孔伸出于针筒外侧，在针筒盖的侧壁上固定连接有支撑架，通过支撑架的设置，可以将针筒盖直立防止在平面上，防止针筒盖被污染。 【EN】The disposable acupuncture point of cylinder formula pastes preparation appearance, including the cylinder, split formula push rod and cylinder lid, split formula push rod slidable mounting is in the cylinder, split formula push rod can be used for stirring traditional chinese medicine, also can be used for pushing away traditional chinese medicine outside the cylinder, the removable tip of installing at the cylinder of cylinder lid, be equipped with the through-hole in the cylinder lid, split formula push rod stretches out in the cylinder outside through the through-hole, fixedly connected with support frame on the lateral wall of cylinder lid, through the setting of support frame, can prevent vertically on the plane with the cylinder lid, prevent that the cylinder lid from being contaminated by the lid.

摘要：【中文】本发明涉及一种木糖母液中多种单糖组分的快速定量方法，利用色谱分析方法对木糖母液待测样品中葡萄糖、木糖、半乳糖、阿拉伯糖和甘露糖共五种组分同时进行含量测定和分析，实现一次性完成木糖母液中的各组分分析，各组分物质峰型尖锐，分离效果显著；在25min内完成分析，分析时间短；所使用的设备较常见，流动相配制简单，方法容易实现，检测成本相对较低。通过标准溶液外标法进行计算，得到木糖母液中各组分的含量。该方法得出的标准曲线的线性关系良好，精密度和准确度高，测定结果可靠，检测效率高，可批量操作。 【EN】The invention relates to a method for rapidly quantifying various monosaccharide components in xylose mother liquor, which is characterized in that five components including glucose, xylose, galactose, arabinose and mannose in a sample to be tested in the xylose mother liquor are simultaneously subjected to content measurement and analysis by using a chromatographic analysis method, so that the analysis of each component in the xylose mother liquor is completed at one time, the substance peak of each component is sharp, and the separation effect is obvious; the analysis is completed within 25min, and the analysis time is short; the used equipment is common, the mobile phase is simple to prepare, the method is easy to realize, and the detection cost is relatively low. And calculating by a standard solution external standard method to obtain the content of each component in the xylose mother liquor. The standard curve obtained by the method has good linear relation, high precision and accuracy, reliable measuring result, high detection efficiency and batch operation.

摘要：【中文】本发明涉及分子生物学技术领域，具体涉及一种牛诺如病毒的实时荧光定量RT‑PCR检测方法。该方法是基于BNoV的靶向RdRp基因设计一对特异性引物，并对实时荧光定量PCR的反应体系及反应条件进行优化，从而建立的检测BNoV的荧光定量PCR方法，该方法线性关系良好，扩增效率好；是常规PCR方法敏感性的1000倍，敏感性较高；仅对BNoV具有良好的扩增曲线，特异性好；组内和组间差异均较小，具有良好的可重复性。 【EN】The invention relates to the technical field of molecular biology, in particular to a real-time fluorescent quantitative RT-PCR detection method of a norovirus. The method is a fluorescence quantitative PCR method for detecting BNoV, which is established by designing a pair of specific primers based on a targeted RdRp gene of BNoV and optimizing a reaction system and reaction conditions of real-time fluorescence quantitative PCR, and has good linear relation and good amplification efficiency; the sensitivity is 1000 times that of the conventional PCR method, and the sensitivity is higher; the primer has a good amplification curve only for BNoV, and has good specificity; the difference between groups is small, and the repeatability is good.