摘要：【中文】本发明涉及肌醇的酶催化制备领域，具体的说是一种纤维素完全磷酸解生产肌醇的酶法制备方法。本发明所公开的肌醇的制备方法是以预处理过的纤维素(主要成分为纤维多糖)为酶催化反应的底物；采用纤维多糖磷酸化酶、纤维二糖磷酸化酶、聚磷酸葡萄糖激酶、葡萄糖磷酸变位酶、肌醇‑1‑磷酸合成酶和肌醇单磷酸酶催化纤维多糖完全磷酸解转化为肌醇。通过优化多酶反应过程，分阶段控制反应过程温度，显著提升底物的转化效率和肌醇的产率，缩短了反应时间。该技术方法实现了纤维素底物的完全磷酸解，还可以用于其他体外多酶反应体系催化纤维多糖产氢气、产电或生产其他生物基化学品。 【EN】The invention relates to the field of enzyme-catalyzed preparation of inositol, in particular to an enzymatic preparation method for producing inositol by completely phosphorylating cellulose. The preparation method of the inositol disclosed by the invention takes pretreated cellulose (the main component is cellopolysaccharide) as a substrate of enzyme catalytic reaction; the complete phosphorylation conversion of the fibrous polysaccharide into inositol is catalyzed by fibrous polysaccharide phosphorylase, cellobiose phosphorylase, polyphosphoric acid glucokinase, glucose phosphoglucomutase, inositol-1-phosphate synthase and inositol monophosphate enzyme. By optimizing the multienzyme reaction process and controlling the temperature of the reaction process in stages, the conversion efficiency of the substrate and the yield of inositol are obviously improved, and the reaction time is shortened. The technical method realizes the complete phosphorolysis of the cellulose substrate, and can also be used for catalyzing fibrous polysaccharide to produce hydrogen and electricity or produce other bio-based chemicals by other in-vitro multi-enzyme reaction systems.

摘要：【中文】本发明公开了一种利用微生物全细胞和/或其裂解液催化肌肉肌醇制备鲨肌醇的方法，包括构建表达耐热肌肉肌醇脱氢酶和/或耐热鲨肌醇脱氢酶基因的工程菌，并将工程菌进行细胞膜通透性处理和/或破碎，然后利用透性工程菌或其裂解液将肌肉肌醇转化为鲨肌醇。本发明的方法与现有生产鲨肌醇的方法相比，具有生产工艺简单、生产成本低、产率高等优点。 【EN】The invention discloses a method for preparing scyllo-inositol by catalyzing myoinositol with whole cells of microorganisms and/or lysate thereof, which comprises the steps of constructing engineering bacteria for expressing thermotolerant myoinositol dehydrogenase and/or thermotolerant scyllo-inositol dehydrogenase genes, carrying out cell membrane permeability treatment and/or disruption on the engineering bacteria, and then converting the myoinositol into the scyllo-inositol by utilizing the permeable engineering bacteria or the lysate thereof. Compared with the existing method for producing scyllo-inositol, the method of the invention has the advantages of simple production process, low production cost, high yield and the like.

摘要：【中文】本发明公开了一种“一锅‑两步”酶催化法由肌醇制备葡萄糖二酸的方法，包括利用肌醇氧化酶催化肌醇生成中间体葡萄糖醛酸，继而利用醛酸脱氢酶将中间体葡萄糖醛酸转化为葡萄糖二酸。本发明的方法与现有生产葡萄糖二酸的方法相比，具有生产工艺简单、绿色友好、产率高、易于规模化制备等优点。 【EN】The invention discloses a method for preparing glucaric acid from inositol by a one-pot-two-step enzyme catalysis method, which comprises the steps of catalyzing inositol by using inositol oxidase to generate intermediate glucuronic acid, and then converting the intermediate glucuronic acid into glucaric acid by using aldehyde dehydrogenase. Compared with the existing method for producing glucaric acid, the method provided by the invention has the advantages of simple production process, environmental friendliness, high yield, easiness in large-scale preparation and the like.

摘要：【中文】本申请实施例提供了一种音频处理方法、终端及服务器，涉及通信技术领域，该方法包括：终端对采集的音频信号进行语音特征提取，获得语音特征信息，然后将包含语音特征信息的音频数据包发送至服务器。服务器从音频数据包中获得语音特征信息并基于语音特征信息进行选路，从各个音频数据包的编码音频信号中选取目标音频信号，之后再基于选择的目标音频信号进行混音处理。相较于服务器对音频数据包解码后进行语音特征提取来说，终端进行语音特征提取获得的语音特征信息更加真实反映了音频的语音段和非语音段，从而提高服务器选路的准确性。由于服务器不需要对所有的音频数据包进行解码以及不需要进行语音特征提取，从而降低了服务器的消耗。 【EN】The embodiment of the application provides an audio processing method, a terminal and a server, which relate to the technical field of communication, and the method comprises the following steps: the terminal extracts voice characteristics of the collected audio signals to obtain voice characteristic information, and then sends an audio data packet containing the voice characteristic information to the server. The server obtains the voice characteristic information from the audio data packets, performs route selection based on the voice characteristic information, selects a target audio signal from the coded audio signals of each audio data packet, and performs sound mixing processing based on the selected target audio signal. Compared with the voice characteristic extraction after the server decodes the audio data packet, the voice characteristic information obtained by the terminal through the voice characteristic extraction more truly reflects the voice section and the non-voice section of the audio, and therefore the accuracy of the server routing is improved. The server does not need to decode all audio data packets and does not need to extract voice features, so that the consumption of the server is reduced.

摘要：【中文】一些实施例涉及用于制造存储器件的方法。该方法包括形成设置在介电层上方的第一掩模层，第一掩模层具有侧壁，该侧壁限定设置在位于嵌入式存储区域中的磁阻式随机存取存储器(MRAM)单元之上的开口。实施第一蚀刻以在MRAM单元之上的介电层内形成第一通孔开口。在MRAM单元和介电层上方形成顶部电极通孔层。对顶部电极通孔层实施第一平坦化工艺以去除顶部电极通孔层的一部分并且限定具有基本平坦顶面的顶部电极通孔。本发明的实施例还涉及集成电路。 【EN】Some embodiments relate to a method for manufacturing a memory device. The method includes forming a first mask layer disposed over a dielectric layer, the first mask layer having sidewalls that define an opening disposed over a Magnetoresistive Random Access Memory (MRAM) cell located in an embedded memory region. A first etch is performed to form a first via opening within the dielectric layer over the MRAM cell. A top electrode via layer is formed over the MRAM cells and the dielectric layer. A first planarization process is performed on the top electrode via layer to remove a portion of the top electrode via layer and define a top electrode via having a substantially planar top surface. Embodiments of the invention also relate to integrated circuits.