摘要：【中文】本发明公开了一种血清中维生素D代谢物‑25（OH）D3的提取方法及检测方法，该提取方法包括：1）使25（OH）D3从沉淀蛋白中释放出，再瞬离至少一次，从而获得含有25（OH）D3、磷脂和蛋白质的混合物样品；2）利用硅藻土萃取板至少吸附混合物样品中的磷脂和蛋白质；3）对硅藻土萃取板进行洗脱，然后压合硅藻土萃取板和收集板从而收集含有25（OH）D3的洗脱液；4）吹干收集板，并复溶维生素D；该检测方法包括将血清中维生素D代谢物‑25（OH）D3的提取方法所获的25（OH）D3的复溶液作为待检测物；将待检测物上样至液相色谱串联质谱仪检测。本发明提供的提取方法即萃取方法,可同时完成样品富集与净化，萃取率更高,能够大大提高后续检测的灵敏度。 【EN】The invention discloses an extraction method and a detection method of a vitamin D metabolite-25 (OH) D3 in serum, wherein the extraction method comprises the following steps: 1) releasing 25(OH) D3 from the precipitated protein and transiently dissociating at least once to obtain a sample mixture comprising 25(OH) D3, phospholipids and proteins; 2) adsorbing at least phospholipids and proteins in a sample of the mixture using a diatomaceous earth extraction plate; 3) eluting the diatomite extraction plate, and then pressing the diatomite extraction plate and the collection plate together to collect the eluent containing 25(OH) D3; 4) drying the collecting plate by blowing, and re-dissolving vitamin D; the detection method comprises the steps of taking a re-solution of 25(OH) D3 obtained by the extraction method of 25(OH) D3 as a to-be-detected object; and (4) loading the substance to be detected to a liquid chromatogram tandem mass spectrometer for detection. The extraction method provided by the invention can complete sample enrichment and purification at the same time, has higher extraction rate, and can greatly improve the sensitivity of subsequent detection.

摘要：【中文】本发明公开了一种血清中维生素D代谢物‑25（OH）D3的萃取方法及检测方法，该萃取方法包括：1）使25（OH）D3从沉淀蛋白中释放出，再瞬离至少一次，从而获得含有25（OH）D3、磷脂和蛋白质的混合物样品；2）利用活性炭萃取板至少吸附混合物样品中的磷脂和蛋白质；3）对活性炭萃取板进行洗脱，然后压合活性炭萃取板和收集板从而收集含有25（OH）D3的洗脱液；4）吹干收集板，并进行复溶；该检测方法包括将血清中维生素D代谢物‑25（OH）D3的萃取方法所获的25（OH）D3的复溶液作为待检测物；将待检测物上样至液相色谱串联质谱仪检测。本发明提供的萃取方法可同时完成样品富集与净化，萃取率更高,能够大大提高后续检测的灵敏度。 【EN】The invention discloses an extraction method and a detection method of a vitamin D metabolite-25 (OH) D3 in serum, wherein the extraction method comprises the following steps: 1) releasing 25(OH) D3 from the precipitated protein and transiently dissociating at least once to obtain a sample mixture comprising 25(OH) D3, phospholipids and proteins; 2) adsorbing at least phospholipid and protein in the mixture sample by using an activated carbon extraction plate; 3) eluting the activated carbon extraction plate, and then pressing the activated carbon extraction plate and the collection plate together to collect the eluent containing 25(OH) D3; 4) drying the collecting plate and redissolving; the detection method comprises the steps of taking a re-solution of 25(OH) D3 obtained by the extraction method of a vitamin D metabolite-25 (OH) D3 in serum as an object to be detected; and (4) loading the substance to be detected to a liquid chromatogram tandem mass spectrometer for detection. The extraction method provided by the invention can simultaneously complete sample enrichment and purification, has higher extraction rate, and can greatly improve the sensitivity of subsequent detection.