摘要：【中文】本发明提供了一种原代肿瘤细胞筛选培养基，所述原代肿瘤细胞筛选培养基由以下体积百分比的成分组成：5％～20％的无细胞腹水、3％～15％的胎牛血清，其余为哺乳动物细胞培养基。本发明提供的筛选培养基成分能够选择性杀伤间质细胞，对肿瘤细胞生长具有高选择性。同时，能够提高肿瘤细胞的增殖速度，缩短培养周期。本发明提供的肿瘤细胞系构建方法可以纯化癌细胞系至99％以上，大大提高了体外构建肿瘤细胞系的成功率。 【EN】The invention provides a primary tumor cell screening culture medium, which consists of the following components in percentage by volume: 5 to 20 percent of acellular ascites, 3 to 15 percent of fetal calf serum and the balance of a mammalian cell culture medium. The screened culture medium component provided by the invention can selectively kill interstitial cells and has high selectivity on the growth of tumor cells. Meanwhile, the proliferation speed of the tumor cells can be improved, and the culture period is shortened. The tumor cell line construction method provided by the invention can purify the cancer cell line to more than 99%, and greatly improves the success rate of in vitro construction of the tumor cell line.

摘要：【中文】本发明公开了一种溶解性有机质污染的污水尾水分级处理方法，该方法包括：对溶解性有机质污染的污水尾水进行过滤预处理，得到中间污水I；将中间污水I依次通过膜孔径为10kDa的第一超滤膜和膜孔径为5kDa的第二超滤膜，进行二级超滤处理，分别得到一级污水、二级污水和三级污水；再分别向各级污水中投加磁粉、絮凝剂和助凝剂，得到的各级出水混合后，得到分级处理后的总出水。本发明通过不同孔径的超滤膜，将溶解性有机质污染的污水尾水分成三级污水，并分别对每级污水进行磁混凝处理，排除不同分子量的溶解性有机质之间的相互影响和干扰，显著提高了磁混凝技术对溶解性有机质污染中氨氮、总磷等主要水污染物的去除效率。 【EN】The invention discloses a method for grading and treating tail water of sewage polluted by soluble organic matters, which comprises the following steps: carrying out filtration pretreatment on the sewage tail water polluted by the dissolved organic matters to obtain intermediate sewage I; sequentially passing the intermediate sewage I through a first ultrafiltration membrane with a membrane aperture of 10kDa and a second ultrafiltration membrane with a membrane aperture of 5kDa, and performing secondary ultrafiltration treatment to respectively obtain primary sewage, secondary sewage and tertiary sewage; and respectively adding magnetic powder, a flocculating agent and a coagulant aid into the sewage at each stage, and mixing the obtained effluent at each stage to obtain the total effluent after grading treatment. According to the invention, the tail water of the sewage polluted by the dissolved organic matters is divided into three stages of sewage through ultrafiltration membranes with different apertures, and magnetic coagulation treatment is respectively carried out on each stage of sewage, so that mutual influence and interference among the dissolved organic matters with different molecular weights are eliminated, and the removal efficiency of the magnetic coagulation technology on main water pollutants such as ammonia nitrogen, total phosphorus and the like in the dissolved organic matter pollution is remarkably improved.