摘要：本发明公开了一株副猪嗜血杆菌MnSOD蛋白制备的杂交瘤细胞株、其分泌的单克隆抗体以及制备的用于检测副猪嗜血杆菌的阻断ELISA试剂盒，所述试剂盒包括包被酶标版，辣根过氧化物酶标记的单克隆抗体，所述杂交瘤细胞株的保藏编号为：CCTCC NO:C202230，所述副猪嗜血杆菌MnSOD蛋白单克隆抗体由上述杂交瘤细胞株所分泌，所述包被酶标板以MnSOD蛋白作为包被抗原。本发明的试剂盒以阻断ELISA法为原理，检测副猪嗜血杆菌，具有灵敏性和特异性高，重复性好，检测快速方便，易于标准化等优点，非常方便。

摘要：【中文】本发明属于生物技术领域，提供一种猪特异性CpG寡聚脱氧核苷酸，包括CpG‑08序列，CpG‑08序列如SEQ ID NO.1所示；还提供了含有上述的一种猪特异性CpG寡聚脱氧核苷酸重复序列的质粒。本发明中构建的pCpG‑08重组质粒对猪瘟活疫苗和猪圆环病毒灭活疫苗的免疫均具有较好的增强作用，可成为新的疫苗佐剂投放市场。 【EN】The invention belongs to the technical field of biology, and provides a pig specificity CpG oligodeoxynucleotide which comprises a CpG-08 sequence, wherein the CpG-08 sequence is shown as SEQ ID NO. 1; also provides a plasmid containing the porcine specific CpG oligodeoxynucleotide repetitive sequence. The pCpG-08 recombinant plasmid constructed in the invention has a good enhancement effect on the immunity of the classical swine fever live vaccine and the porcine circovirus inactivated vaccine, and can be used as a new vaccine adjuvant to be put on the market.