摘要：【中文】本发明涉及一种基于金磁微粒的时间分辨荧光免疫检测方法，属于免疫学检测领域。本发明提供基于金磁微粒的时间分辨荧光免疫检测方法，包括免疫金磁微粒液的制备，用镧系元素鳌合物标记抗原或抗体，获得荧光标记物，将待检测样品加入免疫金磁微粒液中，再荧光标记物，混匀孵育，然后洗涤金磁微粒，反应体系中加入荧光增强物，然后检测反应体系中标记物的荧光。本发明提供的基于金磁微粒的时间分辨荧光免疫检测方法，首次将金磁微粒包被生物原料技术用于时间分辨荧光检测，大大提高检测范围，缩短反应时间，提高灵敏度，金磁微粒可以和不同的探针分子结合，使检测同一样本中不同的待测物成为可能。 【EN】The invention relates to a time-resolved fluorescence immunoassay method based on gold magnetic particles, and belongs to the field of immunological detection. The invention provides a time-resolved fluorescence immunoassay method based on gold magnetic particles, which comprises the steps of preparing an immune gold magnetic particle liquid, labeling an antigen or an antibody by using lanthanide chelate to obtain a fluorescence label, adding a sample to be detected into the immune gold magnetic particle liquid, then uniformly mixing and incubating the fluorescence label, then washing the gold magnetic particles, adding a fluorescence enhancer into a reaction system, and then detecting the fluorescence of the label in the reaction system. The gold magnetic particle-coated biological raw material technology is used for time-resolved fluorescence detection for the first time, so that the detection range is greatly improved, the reaction time is shortened, the sensitivity is improved, and the gold magnetic particles can be combined with different probe molecules, so that the detection of different substances to be detected in the same sample is possible.