摘要：【中文】本发明公开了一种强酸性条件下基质附着生物膜中微生物总DNA的提取方法，属于分子生物学技术领域。包括以下步骤：1)采用pH＝4～5的缓冲液1在适宜水压下将微生物从附着基质上洗脱，离心收集菌体样品；2)采用含有EDTA的pH＝7～7.5的缓冲液2进行洗涤、离心收集菌体样品；3)将步骤2)收集的菌体样品进行DNA提取。本发明的方法提取的微生物总核糖核酸质量较好，采用1％琼脂糖凝胶电泳检测得到的总条带明亮清晰，上机测序时DNA链长和浓度符合测序和建库要求，为强酸性条件下微生物生态学和分子生物学研究奠定了技术基础。 【EN】The invention discloses a method for extracting total DNA of microorganisms in a substrate-attached biomembrane under a strong acid condition, and belongs to the technical field of molecular biology. The method comprises the following steps: 1) eluting microorganisms from the attachment matrix by using a buffer solution 1 with the pH value of 4-5 under proper water pressure, and centrifugally collecting a thallus sample; 2) washing with a buffer solution 2 containing EDTA and having a pH value of 7-7.5, and centrifuging to collect a thallus sample; 3) extracting DNA from the thallus sample collected in the step 2). The microbial total ribonucleic acid extracted by the method has good quality, the total band obtained by detecting the method by adopting 1 percent agarose gel electrophoresis is bright and clear, the length and the concentration of the DNA chain meet the requirements of sequencing and library building when the DNA is subjected to on-machine sequencing, and the method lays a technical foundation for microbial ecology and molecular biology research under the strong acid condition.

摘要：【中文】本发明公开了一种提取基质附着微生物总DNA的装置和方法，属于分子生物学技术领域，该装置包括控压冲洗装置、负压装置和收集装置，通过控压冲洗装置控制冲洗出水水压，通过负压管路将冲洗管路冲洗基质产生的混悬液收集至收集装置，本发明同时公开了DNA的提取方法，在加压下冲洗基质，配合负压装置收集样品，克服了现有技术针对附着在基质上的微生物样品存在取样困难、取样不完整的难题，显著改善了基质附着微生物基因组DNA的代表性、提取效率和质量，条带明亮清晰、基本无降解，对难提取微生物，如真菌等均有较好的提取效率。 【EN】The invention discloses a device and a method for extracting total DNA of matrix-attached microorganisms, belonging to the technical field of molecular biology.

摘要：【中文】本发明公开了一种基于视觉检测系统的自动化漆包线输送线，包括，搬运单元，包括第一搬移组件以及设置于所述第一搬移组件内侧的第一转运组件和第一输送机，所述第一转运组件设置于第一输送机一侧；检测单元，包括设置于所述第一输送机上的视检组件，以及位于第一输送机一端的质检组件；预包装单元，与质检组件连接；以及，装箱单元，设置于预包装单元一端；第一转运组件包括载台和链条输送机，所述载台设置于所述链条输送机上；本发明通过设置的搬运单元、检测单元、预包装单元和装箱单元之间的相互配合，能够实现全自动包装漆包线，提高了漆包线生产自动化水平，操作过程省时省力，大大提高了漆包线生产效率及成本。 【EN】The invention discloses an automatic enameled wire conveying line based on a visual inspection system, which comprises a conveying unit, a conveying unit and a conveying unit, wherein the conveying unit comprises a first conveying assembly, a first transferring assembly and a first conveyor, the first transferring assembly and the first conveyor are arranged on the inner side of the first conveying assembly, and the first transferring assembly is arranged on one side of the first conveyor; the detection unit comprises a visual inspection assembly arranged on the first conveyor and a quality inspection assembly positioned at one end of the first conveyor; the pre-packaging unit is connected with the quality inspection component; the packing unit is arranged at one end of the pre-packaging unit; the first transfer assembly comprises a carrying platform and a chain conveyor, and the carrying platform is arranged on the chain conveyor; according to the invention, through the mutual cooperation of the carrying unit, the detection unit, the pre-packaging unit and the packing unit, the full-automatic enameled wire packaging can be realized, the automatic level of enameled wire production is improved, the operation process is time-saving and labor-saving, and the enameled wire production efficiency and cost are greatly improved.

摘要：【中文】本发明公开了一种地铁车站附属结构，其特征在于：包括顶管机外壳（6）、壳内二衬（7）、暗挖部分、附属结构顶管部分（4）和附属结构明挖部分（3）；暗挖部分一端与车站主体（1）连接，另一端连接壳内二衬（7），顶管机外壳（6）与附属结构顶管部分（4）连接；附属结构顶管部分（4）与附属结构明挖部分（3）连接，所述的顶管机外壳（6）包覆在壳内二衬（7）外表面。 【EN】The invention discloses an auxiliary structure of a subway station, which is characterized in that: comprises a pipe jacking machine shell (6), a shell inner two-lining (7), a subsurface excavated part, an accessory structure pipe jacking part (4) and an accessory structure open excavated part (3); one end of the underground excavation part is connected with the station main body (1), the other end of the underground excavation part is connected with the second lining (7) in the shell, and the shell (6) of the push bench is connected with the push bench part (4) of the accessory structure; the accessory structure pipe jacking part (4) is connected with the accessory structure open cut part (3), and the pipe jacking machine shell (6) is coated on the outer surface of the shell inner two liners (7).