摘要：【中文】本发明涉及二乙酸镳草镰刀菌烯醇竞争型气压免疫传感器及检测方法。二乙酸镳草镰刀菌烯醇竞争型气压免疫传感器，包括单独存放的抗二乙酸镳草镰刀菌烯醇单克隆抗体、酶标条、包被酶标条用二乙酸镳草镰刀菌烯醇‑半谷氨酸‑乳清蛋白偶联物、过氧化氢溶液、表面修饰羊抗鼠抗体的铂纳米粒子或金核铂壳纳米粒子和用于检测气压的气压检测传感器；所述抗二乙酸镳草镰刀菌烯醇单克隆抗体由保藏编号为CCTCC NO:C201881的杂交瘤细胞菌株DAS5G11E7产生。该方法具有灵敏度高、便携、廉价和易于操作等优点。 【EN】The invention relates to a ribes diacetate sickle enol competition type air pressure immunosensor and a detection method. The ribes diacetate sickle knife enol competitive type air pressure immunosensor comprises a ribes diacetate sickle enol monoclonal antibody, an enzyme label strip, a ribes diacetate sickle enol-half glutamic acid-lactalbumin conjugate for coating the enzyme label strip, a hydrogen peroxide solution, platinum nanoparticles or gold core platinum shell nanoparticles with surface modified goat anti-mouse antibodies and an air pressure detection sensor for detecting air pressure, wherein the ribes diacetate sickle enol monoclonal antibody is independently stored; the anti-ribes diacetate fusarenol monoclonal antibody is generated by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO: C201881. The method has the advantages of high sensitivity, portability, low cost, easy operation and the like.

摘要：【中文】本发明涉及同步检测二乙酸镳草镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、T‑2毒素胶体金免疫层析试纸条。它包括底板，底板的一面从上到下依次粘贴吸水垫、检测垫、金标垫和样品垫，相邻各垫在连接处交叠连接，检测垫以硝酸纤维素膜为基垫，硝酸纤维素膜上横向设置有质控线和检测线，检测线位于质控线的下方，个数为三条，呈间隔分布，三条检测线上分别包被有各毒素蛋白偶联物，所述质控线包被有兔抗鼠多克隆抗体；金标垫横向喷涂有胶体金标记的各抗体；所述抗二乙酸镳草镰刀菌烯醇单克隆抗体由保藏编号为CCTCC NO:C201881的杂交瘤细胞株DAS5G11E7分泌产生。能在一条试纸条上实现对二乙酸镳草镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、T‑2毒素三种真菌毒素的同步、快速检测。 【EN】The invention relates to a colloidal gold immunochromatographic test strip for synchronously detecting sickle knife enol, deoxynivalenol and T-2 toxin of ribes diacetate. The detection pad takes a nitrocellulose membrane as a base pad, quality control lines and detection lines are transversely arranged on the nitrocellulose membrane, the detection lines are positioned below the quality control lines, the number of the detection lines is three, the detection lines are distributed at intervals, each toxin protein conjugate is respectively coated on each detection line, and the quality control lines are coated with rabbit anti-mouse polyclonal antibodies; the gold label pad is transversely sprayed with each antibody marked by colloidal gold; the anti-ribes diacetate fusarenol monoclonal antibody is secreted and generated by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO: C201881. The method can realize synchronous and rapid detection of three fungaltoxins, namely sickle enol diacetate, deoxynivalenol and T-2 toxin, on one test strip.

摘要：【中文】本发明公开了一种电化学同步检测锌、镉、铅、铜、汞离子的方法。该方法可实现多种重金属离子的高灵敏同步检测，对锌、镉、铅、铜、汞离子的检测灵敏度分别为：0.17,0.51,0.68,0.89,0.51μAμg^‑1L^‑1cm^‑2，检测限分别为0.08,0.09,0.05,0.19,0.01μg/L，线性范围分别为6‑7000,4‑6000,6‑5000,4‑4000,6‑5000μg/L。具有高的灵敏度、良好的选择性、宽的线性范围、优良的重现性、稳定性，达到简单、低廉、快速、灵敏的检测效果。 【EN】The invention discloses a method for electrochemical synchronous detection of zinc, cadmium, lead, copper and mercury ions. The method can realize high-sensitivity synchronous detection of various heavy metal ions, and the detection sensitivity to zinc, cadmium, lead, copper and mercury ions is respectively as follows: 0.17,0.51,0.68,0.89,0.51 μ A μ g^‑1L^‑1cm^‑2The detection limits are respectively 0.08,0.09,0.05,0.19 and 0.01 mu g/L, and the linear ranges are respectively 6-7000,4-6000,6-5000,4-4000 and 6-5000 mu g/L. Has high sensitivity, good selectivity, wide linear range, excellent reproducibility and stabilitySimple, cheap, rapid and sensitive detection effect.