摘要：【中文】本发明公开了一种靶向间充质干细胞外泌体，该靶向间充质干细胞外泌体的配方包括以下组份：间充质干细胞15％‑25％、氨基酸1‑2％、葡萄糖3‑8％、无水氯化钙1‑5％、亮氨酸3‑6％、氯化钾2‑4％、次黄嘌呤1‑2％、胸苷1‑3％、核黄素2‑6％、苯丙氨酸4‑8％、氯化钠0.09‑2％、乳酸钠0.1‑2％、氯化钙0.09‑2％、必须氨基酸0.01‑5％、非必须氨基酸0.01‑5％、人血清白蛋白0.1‑5％，其余为去离子水；本发明还公开了靶向间充质干细胞外泌体的制备方法及其应用，该制备方法包括以下步骤：定量称取，间充质干细胞培养，除菌，囊泡颗粒分离，重悬处理，获取分泌体制剂，注射使用；本发明制备出的靶向间充质干细胞外泌体得到较大提升，在其制备过程中通过除菌级亲水性过滤器对其培养基的上清液杀菌处理，避免其滋生有害细菌。 【EN】The invention discloses a targeting mesenchymal stem cell exosome, which comprises the following components in part by weight: 15-25% of mesenchymal stem cells, 1-2% of amino acid, 3-8% of glucose, 1-5% of anhydrous calcium chloride, 3-6% of leucine, 2-4% of potassium chloride, 1-2% of hypoxanthine, 1-3% of thymidine, 2-6% of riboflavin, 4-8% of phenylalanine, 0.09-2% of sodium chloride, 0.1-2% of sodium lactate, 0.09-2% of calcium chloride, 0.01-5% of essential amino acid, 0.01-5% of non-essential amino acid, 0.1-5% of human serum albumin and the balance of deionized water; the invention also discloses a preparation method and application of the targeted mesenchymal stem cell exosome, wherein the preparation method comprises the following steps: quantitatively weighing, culturing mesenchymal stem cells, sterilizing, separating vesicle particles, carrying out heavy suspension treatment, obtaining a secretion preparation, and injecting for use; the targeted mesenchymal stem cell exosome prepared by the method is greatly improved, and the supernatant of the culture medium is sterilized by a sterilization-grade hydrophilic filter in the preparation process, so that harmful bacteria are prevented from breeding.