摘要：【中文】本发明属于甲醛检测技术领域，具体涉及一种双光子荧光探针、制备方法和在甲醛检测方面的应用。本发明中提供了一种双光子荧光探针，该双光子荧光探针采用氨基胍基作为甲醛活性识别基团，具有灵敏度高，选择性好，响应速度更快，能够应用于活体检测的特点。本发明还提供了该双光子荧光探针的制备方法，羟基四苯乙烯与N‑Boc‑溴乙胺反应，之后脱去Boc，加入S‑甲基异硫氨基脲氢碘酸盐生成产物，即为检测甲醛的荧光探针。 【EN】The invention belongs to the technical field of formaldehyde detection, and particularly relates to a two-photon fluorescent probe, a preparation method and application in the aspect of formaldehyde detection. The invention provides a two-photon fluorescent probe, which adopts aminoguanidino as a formaldehyde activity recognition group, has the characteristics of high sensitivity, good selectivity and higher response speed, and can be applied to in-vivo detection. The invention also provides a preparation method of the two-photon fluorescent probe, which comprises the steps of reacting hydroxy tetraphenylethylene with N-Boc-bromoethylamine, removing Boc, and adding S-methyl isothioaminourea hydroiodide to generate a product, namely the fluorescent probe for detecting formaldehyde.