摘要：【中文】本发明公开了一种强酸性条件下基质附着生物膜中微生物总DNA的提取方法，属于分子生物学技术领域。包括以下步骤：1)采用pH＝4～5的缓冲液1在适宜水压下将微生物从附着基质上洗脱，离心收集菌体样品；2)采用含有EDTA的pH＝7～7.5的缓冲液2进行洗涤、离心收集菌体样品；3)将步骤2)收集的菌体样品进行DNA提取。本发明的方法提取的微生物总核糖核酸质量较好，采用1％琼脂糖凝胶电泳检测得到的总条带明亮清晰，上机测序时DNA链长和浓度符合测序和建库要求，为强酸性条件下微生物生态学和分子生物学研究奠定了技术基础。 【EN】The invention discloses a method for extracting total DNA of microorganisms in a substrate-attached biomembrane under a strong acid condition, and belongs to the technical field of molecular biology. The method comprises the following steps: 1) eluting microorganisms from the attachment matrix by using a buffer solution 1 with the pH value of 4-5 under proper water pressure, and centrifugally collecting a thallus sample; 2) washing with a buffer solution 2 containing EDTA and having a pH value of 7-7.5, and centrifuging to collect a thallus sample; 3) extracting DNA from the thallus sample collected in the step 2). The microbial total ribonucleic acid extracted by the method has good quality, the total band obtained by detecting the method by adopting 1 percent agarose gel electrophoresis is bright and clear, the length and the concentration of the DNA chain meet the requirements of sequencing and library building when the DNA is subjected to on-machine sequencing, and the method lays a technical foundation for microbial ecology and molecular biology research under the strong acid condition.

摘要：【中文】本发明公开了一种提取强酸性条件下基质微生物总RNA和总蛋白的方法，属于分子生物学技术领域。本发明的方法包括以下步骤：1)取基质材料，采用pH＝3.6～5.5的缓冲溶液A在一定压力的水压下冲洗基质材料，将微生物从附着基质表面上洗脱，离心收集菌体；2)将步骤1)收集的菌体裂解，采用溶液B提取总RNA和总蛋白，所述溶液B为pH＝4.0～4.5的Trizol试剂。所述水压压力范围为30～120MPa。本发明提取方法显著改善了强酸性条件下基质附着微生物总RNA和总蛋白的提取效率和质量，满足同时提取或分别提取RNA和蛋白质的检测要求。 【EN】The invention discloses a method for extracting total RNA and total protein of matrix microorganisms under a strong acid condition, and belongs to the technical field of molecular biology. The method of the invention comprises the following steps: 1) taking a matrix material, adopting a buffer solution A with the pH value of 3.6-5.5 to wash the matrix material under the water pressure of certain pressure, eluting microorganisms from the surface of an attached matrix, and centrifugally collecting thalli; 2) cracking the thallus collected in the step 1), and extracting total RNA and total protein by using a solution B, wherein the solution B is a Trizol reagent with the pH value of 4.0-4.5. The water pressure range is 30-120 MPa. The extraction method of the invention obviously improves the extraction efficiency and quality of the total RNA and the total protein of the matrix-attached microorganisms under the strong acid condition, and meets the detection requirements of simultaneously extracting or respectively extracting the RNA and the protein.

摘要：【中文】本发明公开了一种提取基质附着微生物总DNA的装置和方法，属于分子生物学技术领域，该装置包括控压冲洗装置、负压装置和收集装置，通过控压冲洗装置控制冲洗出水水压，通过负压管路将冲洗管路冲洗基质产生的混悬液收集至收集装置，本发明同时公开了DNA的提取方法，在加压下冲洗基质，配合负压装置收集样品，克服了现有技术针对附着在基质上的微生物样品存在取样困难、取样不完整的难题，显著改善了基质附着微生物基因组DNA的代表性、提取效率和质量，条带明亮清晰、基本无降解，对难提取微生物，如真菌等均有较好的提取效率。 【EN】The invention discloses a device and a method for extracting total DNA of matrix-attached microorganisms, belonging to the technical field of molecular biology.

摘要：【中文】本发明公开了一种基于视觉检测系统的自动化漆包线输送线，包括，搬运单元，包括第一搬移组件以及设置于所述第一搬移组件内侧的第一转运组件和第一输送机，所述第一转运组件设置于第一输送机一侧；检测单元，包括设置于所述第一输送机上的视检组件，以及位于第一输送机一端的质检组件；预包装单元，与质检组件连接；以及，装箱单元，设置于预包装单元一端；第一转运组件包括载台和链条输送机，所述载台设置于所述链条输送机上；本发明通过设置的搬运单元、检测单元、预包装单元和装箱单元之间的相互配合，能够实现全自动包装漆包线，提高了漆包线生产自动化水平，操作过程省时省力，大大提高了漆包线生产效率及成本。 【EN】The invention discloses an automatic enameled wire conveying line based on a visual inspection system, which comprises a conveying unit, a conveying unit and a conveying unit, wherein the conveying unit comprises a first conveying assembly, a first transferring assembly and a first conveyor, the first transferring assembly and the first conveyor are arranged on the inner side of the first conveying assembly, and the first transferring assembly is arranged on one side of the first conveyor; the detection unit comprises a visual inspection assembly arranged on the first conveyor and a quality inspection assembly positioned at one end of the first conveyor; the pre-packaging unit is connected with the quality inspection component; the packing unit is arranged at one end of the pre-packaging unit; the first transfer assembly comprises a carrying platform and a chain conveyor, and the carrying platform is arranged on the chain conveyor; according to the invention, through the mutual cooperation of the carrying unit, the detection unit, the pre-packaging unit and the packing unit, the full-automatic enameled wire packaging can be realized, the automatic level of enameled wire production is improved, the operation process is time-saving and labor-saving, and the enameled wire production efficiency and cost are greatly improved.

摘要：【中文】本发明提供一种基于BIM+GIS技术的土方工程虚拟施工方法，利用无人机正向、倾斜摄影技术采集项目地的地形数据，形成资料库；利用BentleyContextCapture，对数据资料进行处理，生成GIS模型；在所述GIS模型中进行现场土石方的开挖与回填测算，输出计算结果；不再停留于传统“方格法”绘制“土方平衡图”进行工程量粗略估算，节约工程量计算时间及提高计算结果的准确度，并且在模型上可以直接进行距离、高层、面积、体积的测量，也可直接计算挖方填方，误差极小。还可用于审核BIM模型测算误差，利用BIM和GIS数据，双重保障土方数据的准确，为合理制定施工计划、精确掌握施工进度以及最终结算提供保障。 【EN】The invention provides an earthwork virtual construction method based on BIM + GIS technology, which utilizes the forward and oblique photography technologies of an unmanned aerial vehicle to collect topographic data of project sites to form a database; processing the data by using a BentleyContextCapture to generate a GIS model; carrying out excavation and backfill measurement and calculation on site earth and stone in the GIS model, and outputting a calculation result; the method does not stay in the traditional 'square method' for drawing the 'earthwork equilibrium diagram' to carry out rough estimation on the engineering quantity, saves the engineering quantity calculation time and improves the accuracy of the calculation result, and can directly measure the distance, the high layer, the area and the volume on a model and also can directly calculate the excavation fill, and the error is extremely small. The method can be used for checking the BIM model to measure and calculate errors, and the accuracy of the earth data is doubly guaranteed by using the BIM and the GIS data, so that guarantee is provided for reasonably making a construction plan, accurately mastering the construction progress and finally settling accounts.

摘要：【中文】本发明涉及一种实现煤炭滚筒干燥系统污染物零排放的工艺及方法，属于节能减排技术领域。技术方案是：干燥机（2）通过烟气管道（8）与旋风除尘器（10）连接后经引风机（11）与湿式除尘器（12）的进风口连接，湿式除尘器（12）的污水排出口通过污水管道（21）与循环水池（14）连接，循环水池（14）经循环水泵（19）与厂区污水处理系统（15）连接后再与湿式除尘器（12）进水口连接，湿式除尘器的排风口与冷却脱水塔（13）连接后，通过净化气管道（18）与助燃风机（6）连接，助燃风机与热风炉（3）连接。本发明的有益效果是：烟气经冷却脱水塔脱水处理后新进入干燥系统的热风炉中进行助燃，干燥系统不再向外排放污染物。 【EN】The invention relates to a process and a method for realizing zero emission of pollutants in a coal roller drying system, belonging to the technical field of energy conservation and emission reduction. The technical scheme is as follows: the drying machine (2) is connected with the cyclone dust collector (10) through a flue gas pipeline (8) and then is connected with an air inlet of a wet dust collector (12) through an induced draft fan (11), a sewage discharge port of the wet dust collector (12) is connected with a circulating water tank (14) through a sewage pipeline (21), the circulating water tank (14) is connected with a factory sewage treatment system (15) through a circulating water pump (19) and then is connected with a water inlet of the wet dust collector (12), an air outlet of the wet dust collector is connected with a cooling dehydration tower (13) and then is connected with a combustion-supporting fan (6) through a purified gas pipeline (18), and the combustion-supporting fan is connected with the hot blast stove (3). The invention has the beneficial effects that: the flue gas is dehydrated by the cooling dehydration tower and then enters a hot blast stove of a drying system for supporting combustion, and the drying system does not discharge pollutants outwards.

摘要：【中文】本发明提供一种基于BIM+GIS的施工场地虚拟构建复原方法，采集项目地的地形数据，形成资料库；对资料库中的数据处理，生成GIS模型；将三维倾斜摄影数据与三维正向摄影数据通过Global Mapper提取坐标与高程信息，合成正像摄影图；结合施工场地三维模型与正向摄影模型，对施工现场需保护或项目完工后需要复原的物体进行坐标、尺寸、形态的进行定位，作为模型数据备份；对施工现场需保护或项目完工后需要复原的物体进行坐标、尺寸、形态的进行定位，为后续加固、保护与复原制定方案与工作提供数据帮助。利用BIM+GIS技术的高精度、可视化、效率高等特点，对施工总平面布置图进行深化设计，辅助项目施工现场实现绿色施工。 【EN】The invention provides a construction site virtual construction restoration method based on BIM + GIS, which is characterized by collecting topographic data of project sites to form a database; processing data in a database to generate a GIS model; extracting coordinate and elevation information from the three-dimensional oblique photography data and the three-dimensional forward photography data through a Global Mapper to synthesize a positive image photographic map; positioning coordinates, sizes and forms of objects needing to be protected on a construction site or needing to be restored after a project is finished by combining a three-dimensional model of the construction site and a forward photography model, and taking the objects as model data backup; and positioning the coordinates, the sizes and the shapes of the objects needing to be protected on the construction site or needing to be restored after the project is finished, and providing data help for subsequent schemes for reinforcing, protecting and restoring and working. The characteristics of high precision, visualization, high efficiency and the like of the BIM + GIS technology are utilized to carry out deep design on the construction general plane layout drawing and assist the project construction site to realize green construction.

摘要：【中文】本发明提供一种基于BIM+GIS的施工场地空间虚拟施工方法，包括如下步骤：步骤1、采集项目地的地形数据，形成资料库；步骤2、对资料库中的数据处理，生成GIS模型；步骤3、在GIS模型中进行空间测量分析；可逆向建模将现有施工现场闲置空闲或利用不合理的空间检查及释放出来，利用空间管理节省空间成本、经济有效的利用空间，最终提供良好工作环境、生活环境。 【EN】The invention provides a construction site space virtual construction method based on BIM + GIS, which comprises the following steps: step 1, collecting topographic data of a project place to form a database; step 2, processing the data in the database to generate a GIS model; step 3, performing spatial measurement analysis in the GIS model; the reversible modeling checks and releases the idle or unreasonable space utilization of the existing construction site, saves space cost by using space management, economically and effectively utilizes space, and finally provides a good working environment and a living environment.

摘要：【中文】本发明提供一种基于BIM+GIS地基与基础分部工程分析及选型方法，采集项目地的地形数据，形成资料库；对资料库中的数据处理，生成GIS地形模型；根据项目地质勘察报告生成BIM地质模型；确定基坑支护的形式、基础的形式，根据相关设计图纸，生成基坑与基础BIM模型；将GIS地形模型与BIM地质模型进行嵌套，修正BIM地质模型的地表偏差，形成BIM修正模型；将基坑与基础BIM模型与BIM修正模型进行二次嵌套形成综合模型，在综合模型上进行快速场内测量计算项目地的土石方计划开挖、回填的工程量，以及进行地基与基础分部工程相关分析及选型，有效把控成本。 【EN】The invention provides a BIM + GIS-based foundation and foundation subsection engineering analysis and model selection method, which is used for collecting topographic data of project sites to form a database; processing data in a database to generate a GIS terrain model; generating a BIM geological model according to the project geological survey report; determining the form of a foundation pit support and the form of a foundation, and generating a BIM (building information modeling) model of the foundation pit and the foundation according to a relevant design drawing; nesting the GIS terrain model and the BIM geological model, and correcting the earth surface deviation of the BIM geological model to form a BIM correction model; and carrying out secondary nesting on the foundation pit and foundation BIM model and the BIM correction model to form a comprehensive model, carrying out rapid in-situ measurement on the comprehensive model to calculate the project amount of planned excavation and backfilling of earth and stone in project sites, and carrying out relevant analysis and type selection on foundation and foundation subsection projects, thereby effectively controlling the cost.

摘要：【中文】本发明公开了一种用于高压架空线路的电磁屏蔽型钢芯铝绞线，包括多股钢导线和通过绞合方式缠绕在所述多股钢导线周围的多股铝导线所制成的绞线本体；每根所述的钢导线和铝导线的外周面均通过电解沉积工艺沉积有用于电磁屏蔽的铁基非晶合金层，所述铁基非晶合金层的组分为Fe：68.77%、Co：18.23%、Si：0.99%、B：12.01%。本发明优点是在现有技术基础上降低了高压架空线路电磁辐射强度50%～70%，减少了对高压架空线路走廊沿线居民和社会人员电磁辐射影响，净化公共空间电磁环境。 【EN】The invention discloses an electromagnetic shielding steel core aluminum stranded wire for a high-voltage overhead line, which comprises a stranded wire body and a plurality of steel wires, wherein the stranded wire body is made of the plurality of aluminum wires wound around the plurality of steel wires in a stranding mode; the periphery of each steel wire and each aluminum wire is deposited with an iron-based amorphous alloy layer for electromagnetic shielding through an electrolytic deposition process, and the iron-based amorphous alloy layer comprises the following components: 68.77%, Co: 18.23%, Si: 0.99%, B: 12.01 percent. The invention has the advantages that the electromagnetic radiation intensity of the high-voltage overhead line is reduced by 50-70% based on the prior art, the electromagnetic radiation influence on residents and social personnel along the corridor of the high-voltage overhead line is reduced, and the electromagnetic environment of a public space is purified.