摘要：【中文】本发明公开了一种固相萃取‑高效液相色谱测定婴幼儿米粉中烟酸、烟酰胺的方法，以EDTA‑NaCl混合溶液为提取液，首次使用亲水亲脂平衡（HLB）固相萃取柱净化，以20 mmol/L甲酸铵（pH 4.5）为流动相，Atlantis T3色谱柱上梯度洗脱分离，在261nm检测紫外吸收的方法。本方法提取效率高、净化效果好、分离度高、分析时间短，线性范围为0.3‑30.0mg/L、检测限（0.3mg/kg）与定量限（1mg/kg）较低、回收率在93.3%~102.5%范围内。方法精密度高、稳定性与重现性好。有较高的实用价值，可应用于各种婴幼儿米粉中烟酸与烟酰胺的测定。 【EN】The invention discloses a method for measuring nicotinic acid and nicotinamide in infant rice flour by using a solid-phase extraction-high performance liquid chromatography, which is a method for detecting ultraviolet absorption at 261nm by using an EDTA-NaCl mixed solution as an extracting solution, using a Hydrophilic Lipophilic Balance (HLB) solid-phase extraction column for purification for the first time, using 20mmol/L ammonium formate (pH 4.5) as a mobile phase and carrying out gradient elution separation on an Atlantis T3 chromatographic column. The method has the advantages of high extraction efficiency, good purification effect, high separation degree, short analysis time, linear range of 0.3-30.0mg/L, lower detection limit (0.3 mg/kg) and quantification limit (1 mg/kg), and recovery rate of 93.3% -102.5%. The method has high precision, stability and reproducibility. Has higher practical value and can be applied to the determination of nicotinic acid and nicotinamide in various infant rice flour.