摘要：【中文】本发明涉及药物化学技术领域，尤其是一种异烟酰胺的制备方法。该种异烟酰胺的制备方法，以固体斜面上保存的丙酸棒杆菌Corynebacteriumpropinquum为出发菌株，经过发酵、膜过滤、水合反应、再过滤、浓缩、干燥、包装的工艺生产异烟酰胺。本发明的一种异烟酰胺的制备方法采用微生物酶催化生产异烟酰胺,具有反应条件温和、选择性好、流程简单、产物产率和纯度高、成本低、环境污染小等优点，生产过程产生的废物少，进一步降低了生产成本，特别适合大规模生产。 【EN】The invention relates to the technical field of medicinal chemistry, in particular to a preparation method of isonicotinamide. The preparation method of the isonicotinamide takes corynebacterium propionicum propinquum stored on a solid inclined plane as an original strain, and produces the isonicotinamide through the processes of fermentation, membrane filtration, hydration reaction, refiltering, concentration, drying and packaging. The preparation method of isonicotinamide adopts microbial enzyme to catalyze and produce isonicotinamide, has the advantages of mild reaction condition, good selectivity, simple process, high product yield and purity, low cost, small environmental pollution and the like, generates less waste in the production process, further reduces the production cost, and is particularly suitable for large-scale production.