摘要：【中文】本发明涉及一种罗非鱼无乳链球菌疫苗及其制备方法，通过生物信息学原理分析和筛选了无乳链球菌的一个表面锚定蛋白的部分多肽序列作为疫苗制备的抗原，并且利用借助化学合成技术构建碳纳米管载表面蛋白系统，将候选出的多肽抗原与功能化单壁碳纳米结合制备罗非鱼无乳链球菌亚单位疫苗。本发明筛选的多肽抗原可比灭活疫苗更为显著地提高被接种的罗非鱼的抗体水平，并可抵抗高毒力的无乳链球菌攻击。并且，本发明筛选的多肽抗原能够紧密、均匀地连接于功能化修饰的碳纳米管表面，与单独的多肽抗原组对比，能够显著提高罗非鱼的免疫力。 【EN】The invention relates to a tilapia streptococcus agalactiae vaccine and a preparation method thereof, wherein a partial polypeptide sequence of a surface anchoring protein of streptococcus agalactiae is analyzed and screened as an antigen for preparing the vaccine by a bioinformatics principle, a carbon nano tube surface protein carrying system is constructed by means of a chemical synthesis technology, and the selected polypeptide antigen is combined with functionalized single-walled carbon nano to prepare the tilapia streptococcus agalactiae subunit vaccine. Compared with an inactivated vaccine, the polypeptide antigen screened by the invention can remarkably improve the antibody level of the inoculated tilapia and can resist the attack of the streptococcus agalactiae with high toxicity. Moreover, the polypeptide antigen screened by the invention can be tightly and uniformly connected to the surface of the carbon nano tube subjected to functional modification, and compared with an independent polypeptide antigen group, the immunity of tilapia can be obviously improved.