摘要：【中文】本发明公开了一种基于外显子测序数据的拷贝数变异检测方法及系统、终端和存储介质。方法包括：对正常样本的外显子测序数据进行数据清理，然后对数据进行标准化处理，得到正常样本集数据矩阵；根据每个外显子区域在所有样本中的离散程度，将外显子区域划分成稳定与不稳定的区域；正常样本集数据矩阵在外显子稳定的区域中处理批次效应进而构建参考数据矩阵；使用PCA方法对参考数据矩阵进行处理，通过用主成分重构原始数据，将参考数据矩阵转换到其他空间并得到新的参数；将测试数据变换到参考数据矩阵使用PCA转换后的空间中，然后使用Z‑score方法得到测试数据与参考数据矩阵在当前空间中的差异程度，完成对测试样本的拷贝数变异的检测。采用该方法可以降低成本，实现外显子测序数据拷贝数变异检测的准确性和有效性。 【EN】The invention discloses a copy number variation detection method and system based on exon sequencing data, a terminal and a storage medium. The method comprises the following steps: performing data cleaning on exon sequencing data of a normal sample, and then performing standardization processing on the data to obtain a normal sample set data matrix; dividing the exon regions into stable and unstable regions according to the discrete degree of each exon region in all samples; processing batch effect of the normal sample set data matrix in an exon stable region to further construct a reference data matrix; processing the reference data matrix by using a PCA method, and transforming the reference data matrix into other spaces by reconstructing original data by using principal components to obtain new parameters; and converting the test data into a space obtained by converting the reference data matrix by using PCA, and then obtaining the difference degree of the test data and the reference data matrix in the current space by using a Z-score method to finish the detection of the copy number variation of the test sample. By adopting the method, the cost can be reduced, and the accuracy and the effectiveness of exon sequencing data copy number variation detection can be realized.

摘要：【中文】一种基于模式增长算法的基因变异检测方法，在预处理后的测序比对数据中提取有变异特征信号的数据并进行聚类，将聚类后的每一类数据中所有的短读段序列依据比对状态拆分成状态分别为S和M的两段，将所有的S段的序列压缩建立一致性序列，将所有的M段的序列压缩建立一致性序列；并对同一类中的数据信息进行计算，则形成超项目，根据每个超项目在基因组上的位置将每个超项目按顺序存入变异信号数据库中；使用模式增长算法，建立断点间的比对关系；建立变异模型，确定变异的类型。本发明直接将过滤后数据库中的所有断点为对象进行比对来获取相互比对关系，从而得到全局比对信息，实现更精准的变异检测效果，对较小或较大变异均有很好的检测效果。 【EN】A gene variation detection method based on a pattern growth algorithm comprises the steps of extracting data with variation characteristic signals from preprocessed sequencing comparison data, clustering, splitting all short read segment sequences in each type of clustered data into two segments with states of S and M respectively according to a comparison state, compressing all sequences of the S segments to establish a consistent sequence, and compressing all sequences of the M segments to establish a consistent sequence; calculating data information in the same class to form superitems, and sequentially storing each superitem into a variation signal database according to the position of each superitem on a genome; establishing a comparison relation between breakpoints by using a pattern growth algorithm; and establishing a variation model and determining the type of variation. According to the invention, all breakpoints in the filtered database are directly compared as objects to obtain the mutual comparison relationship, so that the global comparison information is obtained, a more accurate variation detection effect is realized, and a good detection effect is achieved on small or large variations.

摘要：【中文】面向生物大数据的流式传输与变异实时挖掘系统及方法，传输层读取数据层中的测序数据文件，通过生物数据流式传输算法生成测序数据流，再将测序数据流，发送给计算层；计算层接收来自传输层的实时测序读段数据，根据基于Map Reduce的删除变异实时挖掘算法，实时计算该局部测序区域是否存在删除变异并输出删除变异的左右端点，并将删除变异的左右端点传输给用户层。通过基于Map Reduce的删除变异实时挖掘算法，根据实时接收到的局部测序数据流，即可实时判断局部区域是否存在删除变异，而不需要整个基因组中的上下文信息，实现了对测序数据的解耦，降低了传统测序数据处理算法对计算资源的高要求和高依赖。 【EN】The method comprises the steps that a transmission layer reads a sequencing data file in a data layer, a sequencing data stream is generated through a biological data streaming algorithm, and then the sequencing data stream is sent to a calculation layer; and the calculation layer receives real-time sequencing read data from the transmission layer, calculates whether the local sequencing region has deletion variation or not in real time according to a deletion variation real-time mining algorithm based on Map Reduce, outputs left and right endpoints of the deletion variation, and transmits the left and right endpoints of the deletion variation to the user layer. By adopting the Map Reduce-based deletion variation real-time mining algorithm, whether deletion variation exists in a local region can be judged in real time according to a real-time received local sequencing data stream without context information in the whole genome, so that decoupling of sequencing data is realized, and high requirements and high dependence of a traditional sequencing data processing algorithm on computing resources are reduced.

摘要：【中文】一种软硬一体PVC管的制备方法，包括以下操作步骤：该导管材料包括聚氯乙烯树脂，该材料每包含100重量份作为基础树脂的聚氯乙烯树脂就包含如下组分：5‑20重量份丙烯酸酯类交联剂，10‑30重量份增塑剂，1‑4重量份稳定剂，0.1‑3重量份聚乙烯蜡，0.2‑3重量份抗黄剂；将所含原料准确称量造粒，在挤出机中拉管，得到PVC软管；将得到的PVC软管所需软段使用辐照屏蔽板遮挡，所需硬段经过辐照加工，即得制品；本发明的有益效果是：该方法可在同一根PVC导管上实现硬度不同变化，即同一根PVC管材有一段较软，其余则较硬，该方法工艺简单，加工效率高，成本低廉。 【EN】A preparation method of a rigid-flexible integrated PVC pipe comprises the following operation steps: the material for a catheter comprises a polyvinyl chloride resin, and comprises the following components per 100 parts by weight of the polyvinyl chloride resin as a base resin: 5-20 parts of acrylate crosslinking agent, 10-30 parts of plasticizer, 1-4 parts of stabilizer, 0.1-3 parts of polyethylene wax and 0.2-3 parts of anti-yellowing agent; accurately weighing and granulating the contained raw materials, and drawing a pipe in an extruder to obtain a PVC hose; shielding the soft section required by the obtained PVC hose by using an irradiation shielding plate, and performing irradiation processing on the required hard section to obtain a product; the invention has the beneficial effects that: the method can realize different hardness changes on the same PVC pipe, namely one section of the same PVC pipe is softer, and the rest is harder.

摘要：【中文】一种基于二代测序数据的反转相关复杂变异检测方法，在滑动窗口内，根据给定的bam文件与选定的参考基因组进行比对，得到Read Pair信号，并以Read Pair信号对不能完全匹配的Read进行Split Read信号分析，得到对应的断点匹配情况；建立Split Read信号理论模型；将断点匹配情况经过建立的模型，如果符合某个模型时，记录下相应的变异类型和位置，再判断是否是可信的变异。本发明根据理论信号建立了变异模型信号，因此可以很准确地提出变异类型；本发明使用Split Read信号，以模式增长算法寻找字符串的最大最小唯一子串，所以能够很精确地指出变异的位置信息。 【EN】A reverse correlation complex variation detection method based on second-generation sequencing data is characterized in that in a sliding window, a given bam file is compared with a selected reference genome to obtain a Read Pair signal, and the Read Pair signal is used for performing Split Read signal analysis on Read which cannot be completely matched to obtain a corresponding breakpoint matching condition; establishing a Split Read signal theoretical model; and recording the corresponding mutation type and position if the breakpoint matching condition meets a certain model, and judging whether the mutation is credible. According to the method, the variation model signal is established according to the theoretical signal, so that the variation type can be accurately provided; the invention uses the Split Read signal to search the maximum and minimum unique substrings of the character string by a pattern growth algorithm, so that the position information of the variation can be accurately pointed out.

摘要：【中文】一种轮式磨煤机挡板式静态分离器，包括：锥面外壳、锥面内斗、分配器、落煤管、叶片、叶片操控装置、回粉装置、分离器下端的落煤格栅。所作的改进是：叶片操纵装置使用手摇式手动推杆；在叶片的迎风面设置L导流板；在锥面内斗上口增设叶片转轴下端约束座；由内锥壳、外锥壳、重力闭合排粉门，组成回粉装置。本发明的积极效果是：叶片操纵装置改为手动推杆式，结构简单，操作更加方便，便于调整，转角精确。叶片两端固定，使叶片保持垂直、角度一致性，提高了分离效果，叶片上增加导流板，切向气流比例增加，提升分离效率。回粉机构改为内、外锥壳组合的自重式排粉，收集回落的煤粉效率更高，结构简单、降低了设备的生产制造成本。 【EN】A wheel mill baffled static separator comprising: the device comprises a conical surface shell, a conical surface inner hopper, a distributor, a coal dropping pipe, blades, a blade control device, a powder returning device and a coal dropping grid at the lower end of a separator. The improvement is as follows: the blade control device uses a hand-operated manual push rod; arranging an L-shaped guide plate on the windward side of the blade; a restriction seat at the lower end of a blade rotating shaft is additionally arranged at the upper opening of the conical inner bucket; the powder returning device is composed of an inner cone shell, an outer cone shell and a gravity closed powder discharging door. The invention has the positive effects that: the blade control device is changed into a manual push rod type, so that the blade control device is simple in structure, more convenient to operate, convenient to adjust and accurate in corner. The two ends of each blade are fixed, so that the blades are kept vertical and at the same angle, the separation effect is improved, the guide plates are additionally arranged on the blades, the tangential airflow proportion is increased, and the separation efficiency is improved. The powder returning mechanism is changed into the dead weight type powder discharging of the combination of the inner conical shell and the outer conical shell, the efficiency of collecting the fallen coal powder is higher, the structure is simple, and the production and manufacturing cost of the device is reduced.

摘要：【中文】本发明涉及一种人工挖孔井微型灌注桩支护安全性判定方法，属于桥梁建筑施工领域，通过确定微型灌注桩的施工长度和与竖向线之间的施工角度、施工长度和施工角度、人工挖孔井底的埋深、桩侧土体的饱和重度γ_sat、桩侧处土体的粘聚力和内摩擦角、挖孔井底处的主动土压力强度σ_a0和向下桩身范围内对应一半深度处的主动土压力强度σ_a1、灌注桩远离挖孔井一侧的主动土压力合力、桩体最危险点处的最大剪应力τ_max、桩体最危险点处的弯矩M_max、桩体最危险点处由于弯矩引起的最大拉应力σ_max及对上述结果进行安全性判别确定桩体是否处于安全状态，保证施工安全进行。 【EN】The invention relates to a method for judging the support safety of a miniature cast-in-place pile of an artificial hole digging well, which belongs to the field of bridge building construction and is implemented by determining the construction length of the miniature cast-in-place pileAnd the construction angle between the vertical line and the vertical lineConstruction length and angle, and buried depth of bottom of artificial holeSaturation gravity gamma of soil body on side of pile_satPile side soil mass cohesionAnd angle of internal frictionActive earth pressure intensity sigma at bottom of hole digging well_a0And active soil pressure strength sigma at the position corresponding to half depth in the range of the lower pile body_a1Active soil pressure resultant force of cast-in-place pile far away from hole digging wellMaximum shear stress tau at the most dangerous point of the pile body_maxBending moment M at most dangerous point of pile body_maxMaximum tensile stress sigma caused by bending moment at most dangerous point of pile body_maxAnd judging the safety of the results to determine whether the pile body is in a safe state, so as to ensure the safe construction.