摘要：【中文】本发明公开了一种13型高危HPV多重荧光检测试剂盒，包括热启动Taq酶系统、dUTP‑UDG防污染体系，加尾引物、通用引物、荧光探针、阴性对照和阳性对照，荧光探针为HPV特异性探针；本发明的试剂盒适用于定性检测宫颈脱落细胞和生殖泌尿道分泌物样本中13种可引起宫颈癌的高风险HPV基因型，并对16和18两种基因型进行分型；本试剂盒应用同源加尾系统降低了多个引物之间的干扰，实现了在同一管荧光PCR扩增体系中检测13种HPV基因型，本发明的检测试剂盒，检测通量高，大大降低了筛查成本。 【EN】The invention discloses a 13-type high-risk HPV multiple fluorescence detection kit, which comprises a hot-start Taq enzyme system, a dUTP-UDG anti-pollution system, a tailing primer, a universal primer, a fluorescence probe, a negative control and a positive control, wherein the fluorescence probe is an HPV specific probe; the kit is suitable for qualitatively detecting 13 high-risk HPV genotypes which can cause cervical cancer in cervical exfoliated cell and genitourinary tract secretion samples, and typing 16 and 18 genotypes; the kit reduces the interference among a plurality of primers by applying a homologous tailing system, realizes the detection of 13 HPV genotypes in the same tube fluorescent PCR amplification system, has high detection flux and greatly reduces the screening cost.