摘要：【中文】本发明通过应用所述的番茄果实发育相关基因的功能性分子标记物Sly_miRNA164b，进而对番茄果实发育相关基因进行表达，通过GUS染色技术即可快速验证植株果实的发育状态，操作简单易行，实验结果可靠；同时通过功能性分子标记分析得到Sly_miRNA164b与番茄果实形状和成熟的相关性，能够快速、准确、高效地鉴定番茄果实的形状和成熟，为番茄的筛选提供了依据，同时也为种质资源鉴定中分子标记提供了重要的途径。 【EN】According to the invention, the functional molecular marker Sly _ miRNA164b of the tomato fruit development related gene is applied to express the tomato fruit development related gene, the development state of plant fruits can be rapidly verified by a GUS dyeing technology, the operation is simple and easy, and the experimental result is reliable; meanwhile, the correlation between the Sly _ miRNA164b and the shape and the maturity of the tomato fruit is obtained through functional molecular marker analysis, the shape and the maturity of the tomato fruit can be identified quickly, accurately and efficiently, a basis is provided for screening tomatoes, and an important way is provided for molecular markers in germplasm resource identification.