摘要：【中文】本发明提供了可用于在工业相关条件下不对称合成β‑羟基‑α‑氨基酸的工程化醛缩酶多肽。本公开内容还提供了编码工程化醛缩酶多肽的多核苷酸、能够表达工程化醛缩酶多肽的宿主细胞和使用工程化醛缩酶多肽产生β‑羟基‑α‑氨基酸的方法。与其他制备方法相比，使用本发明的工程化醛缩酶多肽用于β‑羟基‑α‑氨基酸的制备，所得产物中立体异构体纯度高，反应条件温和，具有很好的工业应用前景。 【EN】The present disclosure also provides polynucleotides encoding the engineered aldolase polypeptides, host cells capable of expressing the engineered aldolase polypeptides, and methods of producing β -hydroxy- α -amino acids using the engineered aldolase polypeptides.

摘要：【中文】本发明涉及医药中间体技术领域，具体涉及一种烯酮还原酶与一种布瓦西坦中间体的制备方法，该制备方法包括以下步骤：在吗啉的催化下，戊醛和乙醛酸反应生成5‑羟基‑4‑正丙基‑2(5H)‑呋喃酮；在硼氢化钠的催化下，5‑羟基‑4‑正丙基‑2(5H)‑呋喃酮脱除羟基生成4‑正丙基‑2(5H)‑呋喃酮；在烯酮还原酶的存在下，4‑正丙基‑2(5H)‑呋喃酮发生还原反应生成目标产物布瓦西坦中间体；该烯酮还原酶的核苷酸序列如SEQ ID NO.1所示，且其氨基酸序列如SEQ ID NO.2所示。本发明所述的布瓦西坦中间体的制备方法仅需3步反应，即可制得布瓦西坦中间体，无需额外化学拆分，整体工艺绿色环保，成本较低，易于实施。 【EN】The invention relates to the technical field of medical intermediates, in particular to a preparation method of ketene reductase and a brivaracetam intermediate, which comprises the following steps: under the catalysis of morpholine, valeraldehyde and glyoxylic acid react to generate 5-hydroxy-4-n-propyl-2 (5H) -furanone; under the catalysis of sodium borohydride, hydroxyl of 5-hydroxyl-4-n-propyl-2 (5H) -furanone is removed to generate 4-n-propyl-2 (5H) -furanone; in the presence of ketene reductase, 4-n-propyl-2 (5H) -furanone is subjected to reduction reaction to generate a target product, namely a brivaracetam intermediate; the nucleotide sequence of the enone reductase is shown as SEQ ID NO.1, and the amino acid sequence thereof is shown as SEQ ID NO. 2. The preparation method of the intermediate of the invention can prepare the intermediate of the invention only by 3 steps of reaction without additional chemical resolution, and has the advantages of green and environment-friendly overall process, low cost and easy implementation.