摘要：【中文】本发明公开了一种基于CRISPR/Cas9编辑本氏烟基因的载体及其构建方法和应用，包括针对本氏烟UbEF1B基因构建的BKG‑g10、BKG‑g11载体和/或针对本氏烟CCR4/NOT基因构建的BKG‑g12、BKG‑g13载体。上述载体将UbEF1B基因或CCR4/NOT基因编辑后本氏烟生长状态正常，表型相较于野生型未发生明显变化；不会对其性状产生影响，利于后代的延续。依靠CRISPR/Cas9系统编辑本氏烟内源基因相较于传统的育种方法能更快更有效的获得抗病材料。 【EN】The invention discloses a CRISPR/Cas 9-based vector for editing Nicotiana benthamiana gene, and a construction method and application thereof, wherein the CRISPR/Cas 9-based vector comprises a BKG-g10 and a BKG-g11 vector constructed for the Nicotiana benthamiana UbEF1B gene and/or a BKG-g12 and a BKG-g13 vector constructed for the Nicotiana benthamiana CCR4/NOT gene. The growth state of the Nicotiana benthamiana is normal after the UbEF1B gene or CCR4/NOT gene is edited by the vector, and the phenotype is NOT obviously changed compared with the wild type; the method has no influence on the characters of the plants and is beneficial to the continuation of the offspring. Compared with the traditional breeding method, the method has the advantages that the CRISPR/Cas9 system is relied on to edit the endogenous gene of the Nicotiana benthamiana, so that the disease-resistant material can be obtained more quickly and effectively.