摘要：【中文】本发明公开了一种基于毛囊细胞干性维持的毛发再生剂，包括以下组分：浓度为100.0‑120.0μg/ml的PDGF、浓度为250.0‑350.0μg/ml的KGF、浓度为500.0‑600.0μg/ml的VEGF、浓度为60.0‑80.0μg/ml的间充质干细胞外泌体。本发明的毛发再生剂使脱发部分的毛发得到了再生，并且长出的毛发质地与正常毛发无任何差别。本发明的毛发再生剂治疗脱发效果好，为患者摆脱了脱发的烦恼，具有广阔的应用前景。 【EN】The invention discloses a hair regenerating agent based on hair follicle cell dryness maintenance, which comprises the following components: PDGF with the concentration of 100.0-120.0 mug/ml, KGF with the concentration of 250.0-350.0 mug/ml, VEGF with the concentration of 500.0-600.0 mug/ml and mesenchymal stem cell exosome with the concentration of 60.0-80.0 mug/ml. The hair restorer of the present invention restores hair in the bald part and the hair grows in texture without any difference from normal hair. The hair regenerating agent of the invention has good effect of treating alopecia, gets rid of the trouble of alopecia for patients and has wide application prospect.

摘要：【中文】本发明公开了一种细胞活性检测模拟生理环境缓冲剂，包括以下组分：无机盐、缓冲剂、氨基酸、葡萄糖。所述无机盐包括氯化钠、氯化钾、磷酸二氢钾、碳酸氢钠。所述氨基酸包括L‑精氨酸、L‑门冬氨酸、L‑谷氨酸、甘氨酸、L‑组氨酸、L‑羟脯氨酸、L‑异亮氨酸、L‑亮氨酸、L‑甲硫氨酸、L‑苯丙氨酸、L‑脯氨酸、L‑丝氨酸、L‑苏氨酸、L‑色氨酸、L‑酪氨酸、L‑缬氨酸。所述缓冲剂为4‑羟乙基哌嗪乙磺酸。本发明的细胞活性检测模拟生理环境缓冲剂无毒性，细胞能够很好的保持数量、质量及生物学特性的完整性，充分满足了生物实验的需要，具有广阔的应用前景。 【EN】The invention discloses a cell activity detection simulated physiological environment buffer agent, which comprises the following components: inorganic salt, buffering agent, amino acid and glucose. The inorganic salt comprises sodium chloride, potassium dihydrogen phosphate and sodium bicarbonate. The amino acids include L-arginine, L-aspartic acid, L-glutamic acid, glycine, L-histidine, L-hydroxyproline, L-isoleucine, L-leucine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine, and L-valine. The buffer is 4-hydroxyethyl piperazine ethanesulfonic acid. The buffer agent for simulating the physiological environment in the cell activity detection has no toxicity, and the cells can well keep the completeness of quantity, quality and biological characteristics, fully meet the requirements of biological experiments and have wide application prospects.

摘要：【中文】本发明属于虾捕捞装置技术领域，涉及一种用于渔船的南极磷虾连续性捕捞装置。本发明包括船体和第一捕捞网，第一捕捞网尾部上设有锥形壳体，船体上设有吸虾泵；吸虾泵的吸入端与锥形壳体底部相连，排出端连有磷虾储存舱；锥形壳体内壁上设有螺旋凸起；锥形壳体外壁上设有环形固定架，环形固定架内设有环形滑块，环形滑块上设有若干根叶片连杆，每根叶片连杆上均固设有叶片，若干个所述叶片与环形固定架形成叶轮结构；每根叶片连杆的端部均设有毛刷杆。本发明的优点是：在降低磷虾破损率、提高被捕磷虾的品质时，有效提高网内磷虾的捕捞效率并且及时清理攀附在网尾部上的磷虾。 【EN】The invention belongs to the technical field of shrimp fishing devices, and relates to a euphausia superba continuous fishing device for a fishing boat. The shrimp fishing device comprises a ship body and a first fishing net, wherein a conical shell is arranged at the tail part of the first fishing net, and a shrimp sucking pump is arranged on the ship body; the suction end of the shrimp suction pump is connected with the bottom of the conical shell, and the discharge end of the shrimp suction pump is connected with a krill storage cabin; the inner wall of the conical shell is provided with a spiral bulge; an annular fixing frame is arranged on the outer wall of the conical shell, an annular sliding block is arranged in the annular fixing frame, a plurality of blade connecting rods are arranged on the annular sliding block, blades are fixedly arranged on each blade connecting rod, and the plurality of blades and the annular fixing frame form an impeller structure; the end part of each blade connecting rod is provided with a brush rod. The invention has the advantages that: when reducing the krill breakage rate, improving the quality of catching the krill, effectively improve the efficiency of catching of krill in the net and in time clear up the krill of attaching on the net afterbody.

摘要：【中文】本发明涉及样品检测设备技术领域，具体为一种批量外泌体浓度检测试剂盒及其操作方法，所述试剂盒包括一个恒温箱，所述恒温箱内设有分层测定板，所述分层测定板从上到下分为三层依次为试剂板、样品底板和酶标板底板，所述试剂板、样品底板和酶标板底板一角均设有支架孔，所述试剂板、样品底板和酶标板底板的侧面正对着支架孔处设有固定螺栓，所述支架孔内设有测定板支架并将试剂板、样品底板和酶标板底板依次串联。本发明可快速进行外泌体的整体浓度测量，利用BCA测量原理，掌握了样品整体的外泌体蛋白的浓度参数。 【EN】The invention relates to the technical field of sample detection equipment, in particular to a kit for detecting the concentration of a batch of exosomes and an operation method thereof. The invention can rapidly measure the whole concentration of the exosome, and grasps the concentration parameters of the whole exosome protein of the sample by utilizing the BCA measuring principle.

摘要：【中文】一种基于干细胞上清收集提取鉴定分析的药物制备方法，其特征在于，包括如下步骤：取脐带间充质干细胞，采用完全培养基进行第一培养，然后采用无血清的DMEM/F12培养基进行培养，收集细胞培养基的上清液，得到脐带间充质干细胞上清液；脐带间充质干细胞的接种密度为每平方厘米(1～10)×103个。培养的培养时间为35‑48小时，培养条件为37℃、5％CO2。然后进行第二培养，第二培养的培养时间为40～60小时，培养条件为37℃、5％CO2。所述收集脐带间充质干细胞培养基的上清液之后还包括离心、取上清、提取、鉴定、分析、滤膜过滤的步骤。本发明的药物不会引起任何不良反应，患者容易接受。 【EN】A medicine preparation method based on stem cell supernatant collection, extraction, identification and analysis is characterized by comprising the following steps: taking umbilical cord mesenchymal stem cells, carrying out first culture by adopting a complete culture medium, then carrying out culture by adopting a serum-free DMEM/F12 culture medium, and collecting the supernatant of the cell culture medium to obtain the umbilical cord mesenchymal stem cell supernatant; the inoculation density of the umbilical cord mesenchymal stem cells is 1-10 multiplied by 103 per square centimeter. The culture time is 35-48 hr, and the culture condition is 37 deg.C and 5% CO 2. And then carrying out second culture, wherein the culture time of the second culture is 40-60 hours, and the culture conditions are 37 ℃ and 5% CO 2. The method comprises the following steps of collecting the supernatant of the umbilical cord mesenchymal stem cell culture medium, centrifuging, taking the supernatant, extracting, identifying, analyzing and filtering with a filter membrane. The medicine of the invention can not cause any adverse reaction and is easy to be accepted by patients.

摘要：【中文】本发明公开了一种可变更规模的空调系统及其运行方法。其中空调系统，包括作为末端节点的至少一个室内外和至少一个室外机，还包括：作为中转节点与所述末端节点连接成一个网络的至少一个路由阀；所述至少一个路由阀根据预设策略将网络内的所述室内机的制冷或制热需求传递给至少一个室外机，并将满足所述制冷或制热需求的至少一个室外机和对应的室内机连接形成的冷媒循环回路。本发明可以灵活变更空调系统的规模，使空调系统的规模可以根据需要收缩或扩展。 【EN】The invention discloses a scale-changeable air conditioning system and an operation method thereof. The air conditioning system comprises at least one indoor unit, at least one outdoor unit and at least one outdoor unit, wherein the at least one indoor unit and the at least one outdoor unit are used as end nodes, and the air conditioning system further comprises: at least one routing valve as a transit node connected to said end node in a network; and the at least one routing valve transmits the refrigerating or heating requirements of the indoor units in the network to at least one outdoor unit according to a preset strategy, and connects at least one outdoor unit meeting the refrigerating or heating requirements with the corresponding indoor unit to form a refrigerant circulation loop. The invention can flexibly change the scale of the air conditioning system, so that the scale of the air conditioning system can be contracted or expanded according to requirements.

摘要：【中文】本发明公开了一种毛囊源细胞培养基，包括以下组分和配比：血清替代物 2‑15 v/v%；复合维生素 20‑90 ug/ml；氨基酸 20‑30 ng/ml；细胞生长因子 1.5‑9 ng/ml；L‑谷氨酰胺 3‑7 mmol/ml；青链霉素双抗溶液 12‑18μM；羟基乙醇 18‑25μM；壳聚糖 5‑10μg/ml；倍他米松 10‑15 ng/ml；余量为DMEM/F12培养基。通过本发明培养基培养的毛囊源细胞增长快速，生长稳定，纯度高，安全性高，为毛囊源细胞施用于治疗皮肤缺损奠定了基础，具有很好的临床应用前景。 【EN】The invention discloses a hair follicle source cell culture medium which comprises the following components in parts by weight: 2-15 v/v% serum replacement; vitamin complex 20-90 ug/ml; amino acid 20-30 ng/ml; 1.5-9 ng/ml of cell growth factor; 3-7 mmol/ml of L-glutamine; the streptomycin double-resistant solution is 12-18 mu M; 18-25 mu M of hydroxyl ethanol; 5-10 mug/ml of chitosan; betamethasone 10-15 ng/ml; the balance being DMEM/F12 medium. The hair follicle source cells cultured by the culture medium have the advantages of rapid growth, stable growth, high purity and high safety, lay a foundation for applying the hair follicle source cells to the treatment of skin defects, and have good clinical application prospect.

摘要：【中文】本发明公开了一种毛囊干细胞制备方法，包括以下步骤：剪取志愿者头发毛囊根部，清洗后，加入0.25% Dispase酶，37℃消化0.5‑1.5h；酶消化后，接种于毛囊干细胞培养基中，培养条件为37℃、5% CO₂，每1‑2d换液一次；毛囊干细胞的扩增：将步骤S2获得的毛囊干细胞转接到毛囊干细胞扩增培养基中继续培养；所述毛囊干细胞扩增培养基包括以下组分：DMEM基础培养基、FBS、T‑β、hEGF、VEGF以及Rho相关激酶；毛囊干细胞的纯化。通过本发明方法制备的毛囊干细胞能够长期体外传代培养，并且具有很高的再生和分化能力为毛囊干细胞施用于治疗皮肤缺损奠定了基础，具有很好的临床应用前景。 【EN】The invention discloses a preparation method of hair follicle stem cells, which comprises the following steps: cutting hair follicle root of volunteer, cleaning, adding 0.25% Dispase enzyme, and digesting at 37 deg.C for 0.5-1.5 hr; after enzyme digestion, the cells were inoculated into a hair follicle stem cell medium at 37 ℃ with 5% CO₂The method comprises the following steps of changing the liquid once every 1-2 days, expanding hair follicle stem cells, transferring the hair follicle stem cells obtained in the step S2 into a hair follicle stem cell expansion culture medium for continuous culture, wherein the hair follicle stem cell expansion culture medium comprises the following components of a DMEM (DMEM) basic culture medium, FBS (FBS), T- β, hEGF, VEGF (vascular endothelial growth factor) and Rho-related kinase, and purifying the hair follicle stem cells.

摘要：【中文】本发明涉及生物医药技术领域，一种快速判断样本离体时间试剂，其特征在于其为包含硫化铵或氰化钾的溶液。一种快速判断样本离体时间的方法，其特征包括如下步骤：取离体组织的抗凝血在小三角瓶中摇，如血很快变为鲜红，表明血中含有较多还原血红蛋白；如血不能变红，则可肯定血中仍有其他异常血红蛋白。本发明的快速判断样本离体时间试剂及方法检测手段流程可控，可实现精确检测，具有成本低，实施方便的优点。 【EN】The invention relates to the technical field of biological medicines, and discloses a reagent for quickly judging the in vitro time of a sample. A method for rapidly judging the in vitro time of a sample is characterized by comprising the following steps: the anticoagulation blood of the isolated tissue is taken and shaken in a small triangular flask, if the blood quickly turns into bright red, the blood contains more reduced hemoglobin; if the blood can not turn red, other abnormal hemoglobin still can be ensured in the blood. The reagent and the method for rapidly judging the sample in-vitro time have controllable detection means and flow, can realize accurate detection, and have the advantages of low cost and convenient implementation.

摘要：【中文】本发明公开了一种细胞复苏后高活率冻存液，包括以下组分及含量：海藻糖 6‑10 v/v%；β‑葡聚糖 6‑10 v/v%；羟乙基淀粉 2‑5 v/v%；氨基酸 0.1‑2.0 v/v%；甲基纤维素 0.15‑1.5 v/v%；保护剂 0.18‑0.25 v/v%；甘油 16‑20 v/v%；余量为基础培养基。本发明的细胞复苏后高活率冻存液可以增加复苏后细胞的存活率达90％以上，保存于本发明细胞复苏后高活率冻存液中的细胞可以快速用于临床治疗，为间充质干细胞进一步应用于临床治疗奠定了基础。 【EN】The invention discloses a high-survival-rate frozen stock solution after cell recovery, which comprises the following components and contents of 6-10 v/v% of trehalose, 6-10 v/v% of β -glucan, 2-5 v/v% of hydroxyethyl starch, 0.1-2.0 v/v% of amino acid, 0.15-1.5 v/v% of methyl cellulose, 0.18-0.25 v/v% of protective agent, 16-20 v/v% of glycerol and the balance of a basic culture medium.