摘要：【中文】本发明公开了一种具有滑动装饰可旋推的隐形门，包括墙体、隐形门框和门体，隐形门框位于墙体的内部，放置槽的内部一段设置有固定块，放置槽的一端设置有电动伸缩杆一，电动伸缩杆一与固定块之间通过连接轴相配合连接，矩形安装架与隐形门框的两端滑槽均通过滑动装置相配合连接，转动杆的上端且位于隐形门框的内部设置有与矩形安装架相活动连接的连动杆，隐形门框底端的一侧地面且靠近滑动装置的一侧设置有定位槽孔，矩形安装架的一侧设置有与定位槽孔相配合连接的卡件装置。有益效果：不仅保证了隐形门无论在关闭或开启状态下的美观性及隐形门的整体强度，同时由于施工方法得当，降低了成本，提高了隐形门的使用寿命，易于推广使用。 【EN】The invention discloses a sliding decorative rotatably-pushed invisible door which comprises a wall body, an invisible door frame and a door body, wherein the invisible door frame is positioned in the wall body, a fixed block is arranged at one section in a placing groove, an electric telescopic rod I is arranged at one end of the placing groove, the electric telescopic rod I is matched and connected with the fixed block through a connecting shaft, a rectangular mounting frame is matched and connected with sliding grooves at two ends of the invisible door frame through a sliding device, a linkage rod movably connected with the rectangular mounting frame is arranged at the upper end of a rotating rod and positioned in the invisible door frame, a positioning slotted hole is formed in one side of the bottom end of the invisible door frame, which is the ground and is close to the sliding device, and a clamping device matched and connected with the positioning. Has the advantages that: the method not only ensures the aesthetic property of the invisible door and the integral strength of the invisible door no matter in a closed or opened state, but also reduces the cost, prolongs the service life of the invisible door and is easy to popularize and use due to proper construction method.

摘要：【中文】一种设定级配应用的光触媒涂层的陶瓷制品及其制作方法，包含陶瓷坯体与光触媒涂层，所述光触媒涂层是在创新设计粒径级配并采用磨抛技术嵌铺于烧制后磨抛后的制品表面并在表层成“T”字型平面膜层状态，从而低成本地实现有效且高效、持久地在陶瓷制品表面产生可见光下降解甲醛等有害物质的净化功能作用。 【EN】The ceramic product comprises a ceramic body and a photocatalyst coating, wherein the photocatalyst coating is formed by innovatively designing grain size gradation, embedding and paving the grain size gradation on the surface of a fired polished product by a polishing technology, and forming a T-shaped planar film layer on the surface layer, so that the purification function of effectively, efficiently and durably degrading harmful substances such as formaldehyde under the condition of generating visible light on the surface of the ceramic product is realized at low cost.

摘要：【中文】一种利用磨抛后表面微孔结合纳米材料应用的陶瓷制品及其制作方法，包含陶瓷坯体与预定功能性纳米涂层，所述预定功能性纳米涂层嵌铺于烧制后磨抛后的制品表面并在表层成“T”字型平面膜层状态，从而有效且高效、持久地在陶瓷制品表面释放产生相应预定纳米材料的有益的功能效果。 【EN】The ceramic product comprises a ceramic body and a predetermined functional nano coating, wherein the predetermined functional nano coating is embedded and paved on the surface of the product after being fired and ground and polished and forms a T-shaped plane membrane layer state on the surface layer, so that the beneficial functional effect of the corresponding predetermined nano material is effectively, efficiently and durably released on the surface of the ceramic product.

摘要：【中文】本发明提供了可用于在工业相关条件下不对称合成β‑羟基‑α‑氨基酸的工程化多肽，这些工程化多肽是以催化合成(2S,3R)‑2‑氨基‑3‑羟基‑3‑(4‑硝基苯)丙酸的能力为指标，进过定向进化过程开发而得到的。本公开内容还提供了编码工程化多肽的多核苷酸、能够表达工程化多肽的宿主细胞和使用工程化多肽产生β‑羟基‑α‑氨基酸的方法。与其他制备方法相比，使用本发明的工程化多肽用于β‑羟基‑α‑氨基酸的制备，所得产物中立体异构体纯度高，反应条件温和，污染小，能耗低，因此具有很好的工业应用前景。 【EN】The invention provides engineered polypeptides for asymmetrically synthesizing β -hydroxy- α -amino acid under industrially relevant conditions, which are obtained by developing a directed evolution process with the ability of catalytically synthesizing (2S,3R) -2-amino-3-hydroxy-3- (4-nitrophenyl) propionic acid as an index, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides and a method for producing β -hydroxy- α -amino acid by using the engineered polypeptides.

摘要：【中文】本发明公开了一种大肠杆菌诱导蛋白表达的发酵方法，属于生物技术领域。该发酵方法包括以下步骤：将待发酵的种子液接种于发酵培养液中，并置于0.05~0.1MPa的压力条件下进行恒温发酵培养6~10h后，得到发酵底液；往上述发酵底液中添加诱导剂；所述诱导剂在所述发酵底液中质量浓度为0.1~1.5mmol/L；往上述加有诱导剂的发酵底液中添加补料液，并继续进行恒温发酵培养20~48h，得到发酵产物；所述补料液与所述加有诱导剂的发酵底液的体积比为(0.5~2):1。本发明提供的发酵方法可以在36~40℃的环境下进行诱导蛋白的表达，其无需对发酵底液进行快速降温，也可以达到与降温到25~30℃的蛋白表达量，从而可以大大降低能耗以及节约生产成本。 【EN】The invention discloses a fermentation method for expressing escherichia coli induced protein, and belongs to the technical field of biology. The fermentation method comprises the following steps: inoculating a seed solution to be fermented into a fermentation culture solution, and performing constant-temperature fermentation culture for 6-10 h under the pressure condition of 0.05-0.1 MPa to obtain a fermentation base solution; adding an inducer to the fermentation base solution; the mass concentration of the inducer in the fermentation base solution is 0.1-1.5 mmol/L; adding a feed liquid into the fermentation base liquid added with the inducer, and continuously carrying out constant-temperature fermentation culture for 20-48 h to obtain a fermentation product; the volume ratio of the feed liquid to the fermentation base liquid added with the inducer is (0.5-2): 1. The fermentation method provided by the invention can be used for inducing protein expression in an environment of 36-40 ℃, does not need to rapidly cool the fermentation base liquid, and can reach the protein expression amount of cooling to 25-30 ℃, thereby greatly reducing energy consumption and saving production cost.

摘要：【中文】本发明提供一种工业用酶液的热处理澄清方法，包括以下步骤：(1)选择目的蛋白培养发酵：选择目的蛋白，按照现有发酵生产工艺发酵得到发酵液，再将发酵液进行细胞破碎处理，即得细胞破碎液；(2)热处理澄清：将步骤(1)所得细胞破碎液进行热处理澄清；(3)后处理：将步骤(2)热处理澄清后的液体进行离心处理，并收集清液，即为目的蛋白的工业用酶澄清液。该方法具有工艺简单、成本较低且具有环保优势，澄清后酶液中目的蛋白的酶活损失不大于10％，且浊度低于500NTU并能够通过0.2μm的过滤膜。 【EN】The invention provides a heat treatment clarification method of industrial enzyme liquid, which comprises the following steps: (1) selecting target protein for culture fermentation: selecting target protein, fermenting according to the existing fermentation production process to obtain fermentation liquor, and then performing cell disruption treatment on the fermentation liquor to obtain cell disruption liquid; (2) and (3) heat treatment clarification: carrying out heat treatment clarification on the cell disruption solution obtained in the step (1); (3) and (3) post-treatment: and (3) centrifuging the liquid subjected to heat treatment and clarification in the step (2), and collecting clear liquid, namely the industrial enzyme clarified liquid of the target protein. The method has the advantages of simple process, low cost and environmental protection, the enzyme activity loss of the target protein in the clarified enzyme solution is not more than 10 percent, the turbidity is lower than 500NTU, and the clarified enzyme solution can pass through a filtering membrane of 0.2 mu m.

摘要：【中文】本发明公开了一种光固化3D打印的连续变成分供料出料系统，包括PC机、第一控制器、第二控制器、供料装置、供料管、工作槽、出料管、集料器，工作槽一侧壁设置多个供料口，相对的另一侧壁设置多个出料口，供料装置包括多个进样器，PC机第一控制端与第一控制器连接，第二控制端与第二控制器连接，第一控制器分别与供料装置中的多个进样器电连接，每个进样器分别通过供料管与工作槽的各个供料口连接，工作槽的各个出料口通过出料管与集料器的进料口连接；第二控制器与集料器电连接。本发明在供料过程中通过对供料的成分进行调控，从而使3D打印产品在光固化方向上实现成分的可控变化，可通过成分的变化实现产品功能的多样化和可定制性。 【EN】The invention discloses a continuous-to-separate-feeding and discharging system for photocuring 3D printing, which comprises a PC (personal computer), a first controller, a second controller, a feeding device, a feeding pipe, a working tank, a discharging pipe and a material collector, wherein a plurality of feeding ports are formed in one side wall of the working tank, a plurality of discharging ports are formed in the other opposite side wall of the working tank, the feeding device comprises a plurality of sample injectors, a first control end of the PC is connected with the first controller, a second control end of the PC is connected with the second controller, the first controller is respectively and electrically connected with the plurality of sample injectors in the feeding device, each sample injector is respectively connected with each feeding port of the working tank through the feeding pipe, and each discharging port of the working tank is connected with a feeding port of the material collector through the discharging pipe; the second controller is electrically connected with the material collector. According to the invention, the components of the feeding are regulated and controlled in the feeding process, so that the controllable change of the components of the 3D printing product in the photocuring direction is realized, and the diversification and customizability of the product functions can be realized through the change of the components.

摘要：【中文】本发明公开了一种水气分离装置，包括外壳和分水芯体，分水芯体的上端部和下端部均与外壳的内腔壁密封连接，分水芯体与外壳的内腔壁之间围成分水腔，外壳的内腔中位于上端部以上的部位为排气腔，位于下端部以下的部分为储水腔；外壳上设置有能够与排气腔连通的排气头，能够与储水腔连通的排水头、能够与分水腔连通的进气头，所述下端部上设置有用于连通分水腔和储水腔的导水孔，分水芯体的内部具有通孔，该通孔用于连通储水腔和排气腔。在本发明中，分水腔和排气腔为同心腔结构，相较于现有技术中分水腔和排气腔成横向布置，本发明中的设置方式使得水气分离装置的结构较紧凑，减小了对空间的占用，从而利于整体的合理布局。 【EN】The invention discloses a water-gas separation device, which comprises a shell and a water distribution core body, wherein the upper end part and the lower end part of the water distribution core body are hermetically connected with the inner cavity wall of the shell; be provided with on the shell can with the exhaust head of exhaust chamber intercommunication, can with the drainage head of water storage chamber intercommunication, can with the head of admitting air that divides the water cavity to communicate, be provided with the water guide hole that is used for communicating branch water cavity and water storage chamber under the tip, the inside of dividing the water core has the through-hole, and this through-hole is used for communicating water storage chamber and exhaust chamber. Compared with the prior art that the water dividing cavity and the exhaust cavity are transversely arranged, the water dividing cavity and the exhaust cavity are arranged in a concentric cavity structure, the structure of the water-gas separation device is compact due to the arrangement mode, the space occupation is reduced, and the whole reasonable layout is facilitated.

摘要：【中文】本发明公开了一种道岔模拟操作盘，包括模拟操作电路；所述模拟操作电路包括四个接线端子、2个3档钮子开关、2个2组3位式接点钮子开关、2个直流发光二级管、1个直流电流表头、变压器、电容、电阻、熔断器和整流桥堆；钮子开关K2第二组接点的引脚分别连接钮子开关K1和电容C，上、下两个引脚分别连接两个发光二级管；钮子开关K2第一组接点的中间引脚连接开关K1第一组接点的中间引脚，上、下引脚分别连接X1、X2两个接线端子；四个连接柱外接端子用于连接转辙机。本发明可以快速检测出转辙机内部配线是否正确，且使用便捷。 【EN】The invention discloses a switch simulation operation panel, which comprises a simulation operation circuit; the analog operation circuit comprises four wiring terminals, 2 3-gear button switches, 2 groups of 3-bit contact button switches, 2 direct current light emitting diodes, 1 direct current meter head, a transformer, a capacitor, a resistor, a fuse and a rectifier bridge stack; pins of a second group of contacts of the toggle switch K2 are respectively connected with the toggle switch K1 and the capacitor C, and the upper pin and the lower pin are respectively connected with the two light emitting diodes; the middle pin of the first group of the connection points of the button switch K2 is connected with the middle pin of the first group of the connection points of the switch K1, and the upper pin and the lower pin are respectively connected with two wiring terminals X1 and X2; and the four connecting column external terminals are used for connecting a switch machine. The invention can quickly detect whether the internal wiring of the switch machine is correct or not, and is convenient and fast to use.

摘要：【中文】本发明提供了可用于在工业相关条件下不对称合成β‑羟基‑α‑氨基酸的工程化醛缩酶多肽。本公开内容还提供了编码工程化醛缩酶多肽的多核苷酸、能够表达工程化醛缩酶多肽的宿主细胞和使用工程化醛缩酶多肽产生β‑羟基‑α‑氨基酸的方法。与其他制备方法相比，使用本发明的工程化醛缩酶多肽用于β‑羟基‑α‑氨基酸的制备，所得产物中立体异构体纯度高，反应条件温和，具有很好的工业应用前景。 【EN】The present disclosure also provides polynucleotides encoding the engineered aldolase polypeptides, host cells capable of expressing the engineered aldolase polypeptides, and methods of producing β -hydroxy- α -amino acids using the engineered aldolase polypeptides.