摘要：【中文】本发明公开了猪血浆及肺泡液内艾地普林和磺胺甲噁唑的定量检测方法，包括准备艾地普林和磺胺甲噁唑标准品溶液和待检测的样品提取液；线性回归分析，绘制标准曲线；分别用回收率和变异系数表示准确度和精密度；绘制工作曲线并计算出艾地普林和磺胺甲噁唑在猪血液和肺泡灌洗液中的回归方程和相关系数；实际样品测定和结果计算；本发明方法准确度高，精确度和灵敏度好，杂质较少，针对以往的HPLC法进行了改进，将梯度的时间以及条件进行了优化，增加了各峰之间的分离度，使得目标药物峰的灵敏度增高，提高了回收率。 【EN】The invention discloses a quantitative detection method of adefovir dipivoxil and sulfamethoxazole in pig plasma and alveolar fluid, which comprises the steps of preparing an adefovir dipivoxil and sulfamethoxazole standard solution and a sample extracting solution to be detected; performing linear regression analysis, and drawing a standard curve; the accuracy and the precision are respectively expressed by the recovery rate and the variation coefficient; drawing a working curve and calculating a regression equation and a correlation coefficient of the adefovir dipivoxil and the sulfamethoxazole in the pig blood and the alveolar lavage fluid; actual sample measurement and result calculation; the method has the advantages of high accuracy, good precision and sensitivity and less impurities, improves the conventional HPLC method, optimizes the gradient time and conditions, increases the separation degree among all peaks, increases the sensitivity of the target drug peak and improves the recovery rate.