摘要：【中文】本发明公开了一种载有生物活性物质的聚(β‑氨基酯)药物输送体系及其制备方法，以聚(β‑氨基酯)为主链，疏水性药物与聚(β‑氨基酯)通过点击化学反应偶联到主链上，通过亲疏水力的作用形成不同尺寸的纳米微球，纳米微球体系能够更好穿透组织，能够更好的起到治疗效果。亲水性药物与聚(β‑氨基酯)通过点击化学反应偶联到主链上，由于聚(β‑氨基酯)主链上聚乙二醇能够延长生物活性物质(多肽，抗体，药物)的半衰期，提高生物活性物质的生物利用度。本发明提供了一种简洁快速制备多肽，蛋白，亲疏水型药物载体的制备方法，可以用药物治疗，组织损伤修复和药物缓释领域，例如骨组织工程，神经组织工程，肿瘤治疗，心脑血管治疗等领域。 【EN】The invention discloses a poly (β -amino ester) drug delivery system carrying bioactive substances and a preparation method thereof, wherein poly (β -amino ester) is taken as a main chain, a hydrophobic drug and poly (β -amino ester) are coupled to the main chain through click chemical reaction, and nano microspheres with different sizes are formed under the action of hydrophilic and hydrophobic forces, and the nano microsphere system can better penetrate tissues and can better play a role in treatment.