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申请号:201911355405.6 公开号:CN111024667A 主分类号:G01N21/64
申请人:【中文】中国林业科学研究院【EN】CHINESE ACADEMY OF FORESTRY 申请日:2019.12.25 公开日:2020.04.17
摘要:【中文】本发明涉及一种高通量快速评价和/或筛选耐盐碱植物的方法,其包括如下步骤:1)获得受试植物的离体枝条,得到受试材料;2)将所述受试材料置于盐和/或碱的溶液中进行处理,得到处理材料;同时将所述受试材料置于清水中进行处理,得到空白对照材料;3)测定所述处理材料和所述空白对照材料的叶绿素含量和/或叶绿素荧光参数;4)比较所述处理材料的叶绿素含量和/或叶绿素荧光参数和所述空白对照材料的叶绿素含量和/或叶绿素荧光参数。 【EN】The invention relates to a method for quickly evaluating and/or screening saline-alkali tolerant plants in high throughput, which comprises the following steps: 1) obtaining an isolated branch of a tested plant to obtain a tested material; 2) placing the tested material in a solution of salt and/or alkali for treatment to obtain a treated material; meanwhile, the tested material is placed in clear water for processing, and a blank control material is obtained; 3) determining the chlorophyll content and/or chlorophyll fluorescence parameters of the treated material and the blank control material; 4) comparing the chlorophyll content and/or chlorophyll fluorescence parameter of the treated material with the chlorophyll content and/or chlorophyll fluorescence parameter of the blank control material.
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