摘要：【中文】本发明公开了一种土壤中放线菌原位培养的方法，通过土壤样品2025份和培养基225份混合灭菌，制备培养基质，后对250份土样进行稀释后，倒板培养，可以得到由单个放线菌个体生长而成菌落，建立了一种放线菌有效分离方法，该方法可在保证获得绝大部分放线菌资源信息的情况下、有效减少工作量；且该方法中对土样没有进行热处理、化学处理、添加抗生素等方法，去除环境中占优势但非目标菌株，因此避免了常规方法中易破坏微生物生态环境，实现了放线菌的原位培养,增加了可培养放线菌数量，在放线菌分离技术上取得重要进展，在放线菌资源的保护和发展方面具有广泛的适用性。 【EN】The invention discloses a method for in-situ culture of actinomycetes in soil, which comprises the steps of mixing 2025 parts of a soil sample and 225 parts of a culture medium for sterilization, preparing a culture medium, diluting 250 parts of the soil sample, and performing plate inversion culture to obtain a bacterial colony formed by individual growth of a single actinomycetes; in addition, the method does not carry out heat treatment, chemical treatment, antibiotic addition and other methods on the soil sample, and removes dominant but non-target strains in the environment, thereby avoiding the damage to the microbial ecological environment easily in the conventional method, realizing the in-situ culture of the actinomycetes, increasing the number of the culturable actinomycetes, making an important progress on the actinomycetes separation technology, and having wide applicability in the aspects of the protection and development of actinomycetes resources.