摘要：【中文】本发明公开了一种13型高危HPV多重荧光检测试剂盒，包括热启动Taq酶系统、dUTP‑UDG防污染体系，加尾引物、通用引物、荧光探针、阴性对照和阳性对照，荧光探针为HPV特异性探针；本发明的试剂盒适用于定性检测宫颈脱落细胞和生殖泌尿道分泌物样本中13种可引起宫颈癌的高风险HPV基因型，并对16和18两种基因型进行分型；本试剂盒应用同源加尾系统降低了多个引物之间的干扰，实现了在同一管荧光PCR扩增体系中检测13种HPV基因型，本发明的检测试剂盒，检测通量高，大大降低了筛查成本。 【EN】The invention discloses a 13-type high-risk HPV multiple fluorescence detection kit, which comprises a hot-start Taq enzyme system, a dUTP-UDG anti-pollution system, a tailing primer, a universal primer, a fluorescence probe, a negative control and a positive control, wherein the fluorescence probe is an HPV specific probe; the kit is suitable for qualitatively detecting 13 high-risk HPV genotypes which can cause cervical cancer in cervical exfoliated cell and genitourinary tract secretion samples, and typing 16 and 18 genotypes; the kit reduces the interference among a plurality of primers by applying a homologous tailing system, realizes the detection of 13 HPV genotypes in the same tube fluorescent PCR amplification system, has high detection flux and greatly reduces the screening cost.

摘要：【中文】一种2‑氯‑4‑硝基‑6‑溴‑苯胺的制备方法，包括以下步骤：(a)邻二氯苯进行混酸硝化，生成的废酸继续套用至下一批硝化反应中；(b)硝化产物在醇类溶剂中提纯得3,4‑二氯硝基苯，回收的醇类溶剂继续套用至下一批提纯工序中；(c)3,4‑二氯硝基苯在水相中，采用特定的催化剂进行氨解制得邻氯对硝基苯胺，回收的液氨继续套用至下一批氨解反应中；(d)邻氯对硝基苯胺在溴化氢和氧化剂体系中溴化，溴化废酸液及溴素回收用于下一批溴化反应中。本发明的方法所得产品收率佳，纯度高，质量高，同时对其中两种废酸及溴素实现了回收利用，减少了废水量。与传统工艺相比有明显的质量及环保优势，且生产安全性高。 【EN】A preparation method of 2-chloro-4-nitro-6-bromo-aniline comprises the following steps: (a) carrying out mixed acid nitration on o-dichlorobenzene, and continuously mechanically applying generated waste acid to the next batch of nitration reaction; (b) purifying the nitration product in an alcohol solvent to obtain 3, 4-dichloronitrobenzene, and continuously mechanically applying the recovered alcohol solvent to the next purification process; (c)3, 4-dichloronitrobenzene is subjected to ammonolysis in a water phase by adopting a specific catalyst to prepare o-chloro-p-nitroaniline, and the recovered liquid ammonia is continuously applied to the next batch of ammonolysis reaction; (d) the o-chloro-p-nitroaniline is brominated in a hydrogen bromide and oxidant system, and the bromination waste acid liquor and bromine are recovered and used in the next bromination reaction. The method has the advantages of high product yield, high purity and high quality, and simultaneously realizes the recycling of two waste acids and bromine and reduces the amount of waste water. Compared with the traditional process, the method has the advantages of obvious quality and environmental protection, and high production safety.

摘要：【中文】本发明公开了一种往复式变频深潜泵及其控制方法，属于深潜泵技术领域，将水道设置在打孔的动子内侧，通过启动直线电机使其定子与动子之间相互配合，带动动子上下循环运动，当深井水位达到一定液位时，压力开关或液位计动作，信号传输至模、数转换器，最后将脉冲信号传至地面控制器实施水泵电机与水位高度的连锁作业从而实现PID调节。当水位超过设定值时水泵电机自动启动，水位低于设定值时电机停止运行，从而实现了自动变频调节控制的往复式变频深潜泵来进行抽水或抽油，提高了工作效率降低了能耗。 【EN】The invention discloses a reciprocating variable-frequency deep submersible pump and a control method thereof, belonging to the technical field of deep submersible pumps.A water channel is arranged at the inner side of a perforated rotor, the stator and the rotor are mutually matched by starting a linear motor to drive the rotor to do up-and-down circular motion, when the water level of a deep well reaches a certain liquid level, a pressure switch or a liquid level meter acts, signals are transmitted to an analog-digital converter, and finally pulse signals are transmitted to a ground controller to implement the linkage operation of the water pump motor and the water level height so as to realize PID adjustment. When the water level exceeds the set value, the water pump motor is automatically started, and when the water level is lower than the set value, the motor stops running, so that the reciprocating type variable frequency deep submersible pump controlled by automatic variable frequency regulation is used for pumping water or oil, the working efficiency is improved, and the energy consumption is reduced.