摘要：【中文】本发明公开了一种乳扇成型机，包括机架及设置于机架上的驱动机构、预成型机构、拉伸成型机构和控制装置，控制装置分别与预成型机构和拉伸成型机构形成电性控制连接，驱动机构与预成型机构形成驱动连接；预成型机构包括螺旋挤压装置、进料斗和挤压成型模头，进料斗设置于螺旋挤压装置上，挤压成型模头设置于螺旋挤压装置的出口末端，拉伸成型机构靠近挤压成型模头的输出端，乳品物料由进料斗进入螺旋挤压装置进行螺旋挤压，经螺旋挤压后的乳品物料再经挤压成型模头挤压形成连续的乳片输出，乳片经拉伸成型机构拉伸后，制得乳扇。通过采用预挤出成型与快速拉伸成型相结合的成型结构，实现了乳扇加工的连续化和不同尺寸规格的乳扇产品加工。 【EN】The invention discloses a milk fan forming machine, which comprises a rack, and a driving mechanism, a preforming mechanism, a stretching forming mechanism and a control device which are arranged on the rack, wherein the control device is electrically controlled and connected with the preforming mechanism and the stretching forming mechanism respectively; preforming mechanism includes the screw extrusion device, feeder hopper and extrusion die head, the feeder hopper sets up on the screw extrusion device, the extrusion die head sets up in the export end of screw extrusion device, stretch forming mechanism is close to the output of extrusion die head, the dairy products material gets into the screw extrusion device by the feeder hopper and carries out screw extrusion, the dairy products material after screw extrusion forms continuous emulsion piece output through extrusion die head extrusion again, the emulsion piece is after stretch forming mechanism stretches, make the breast fan. By adopting a forming structure combining pre-extrusion forming and rapid stretching forming, the continuity of the milk fan processing and the milk fan product processing with different sizes and specifications are realized.

摘要：【中文】本发明提供一种重组腺病毒，其包含：目标表达框、位于目标表达框5’端的第一同源臂、位于目标表达框3’端的第二同源臂、一个或两个sgRNA靶标序列，其中sgRNA靶标序列位于第一同源臂的5’端或第二同源臂的3’端，或分别位于第一同源臂的5’端和第二同源臂的3’端；本发明还提供基于所述重组腺病毒的基因编辑方法、制备基因编辑动物的方法、制备工程化T细胞的方法以及包含所述重组腺病毒的系统、组合物和试剂盒。 【EN】The present invention provides a recombinant adenovirus comprising: the target expression cassette comprises a target expression cassette, a first homology arm positioned at the 5 'end of the target expression cassette, a second homology arm positioned at the 3' end of the target expression cassette, and one or two sgRNA target sequences, wherein the sgRNA target sequences are positioned at the 5 'end of the first homology arm or the 3' end of the second homology arm, or positioned at the 5 'end of the first homology arm and the 3' end of the second homology arm respectively; the invention also provides a gene editing method based on the recombinant adenovirus, a method for preparing a gene editing animal, a method for preparing an engineered T cell, and a system, a composition and a kit containing the recombinant adenovirus.

摘要：【中文】一种基于模型拆分与拼接打印的FDM打印方法、系统及介质，属于增材制造技术领域。本发明包括以下步骤：确定待打印产品三维模型的成形方向；以模型中垂直于成形方向且与相邻面外夹角小于180°的平面为拆分面，将模型拆分成若干子模型；将拆分后的子模型全部导入分层软件，根据成形方向与相对位置关系对各子模型进行拼接摆放，随后完成分层切片处理；使用FDM打印机完成产品的增材制造成形。本发明通过模型拆分与拼接打印，可实现FDM打印过程中外表面实体成形区域与内部图形填充区域的层内无相邻打印成形，有利于提升切片层的打印质量与粘结强度，增强FDM打印产品的表面平整度和力学性能。 【EN】An FDM printing method, system and medium based on model splitting and splicing printing belong to the technical field of additive manufacturing. The invention comprises the following steps: determining the forming direction of a three-dimensional model of a product to be printed; taking a plane which is perpendicular to the forming direction and has an included angle of less than 180 degrees with the adjacent plane in the model as a splitting plane, splitting the model into a plurality of sub-models; all split submodels are imported into layering software, all submodels are spliced and placed according to the forming direction and the relative position relation, and then layering slicing processing is finished; and (3) performing additive manufacturing forming of the product by using an FDM printer. According to the invention, through model splitting and splicing printing, adjacent printing forming in the layers of the outer surface entity forming region and the inner graph filling region in the FDM printing process can be realized, the printing quality and the bonding strength of the sliced layer are favorably improved, and the surface flatness and the mechanical property of an FDM printed product are enhanced.

摘要：【中文】本发明提供一种用于超浅覆土层的隧道，该隧道包括若干个沿隧道轴向首尾依次相连的隧道单元，每个隧道单元包括底板、侧板和顶板，侧板的数量为两块，两块侧板设置在底板的两端，顶板设置在侧板的顶端，在底板的内表面形成有第一凹槽，在第一凹槽内部设置有基座，在基座的上表面设置有轨道，在顶板的内部开设有引流通道，在顶板的外表面开设有排水槽，在排水槽内部设置有滤棉，在顶板的内部开设有排水通道，引流通道与排水槽的下部相连通，排水通道与引流通道的下端相连通。该隧道可以解决地表渗水的问题，还可以减轻隧道内部的噪音污染，同时解决了隧道接缝处易扩展的问题。 【EN】The invention provides a tunnel for an ultra-shallow soil covering layer, which comprises a plurality of tunnel units which are sequentially connected end to end along the axial direction of the tunnel, wherein each tunnel unit comprises a bottom plate, two side plates and a top plate, the two side plates are arranged at two ends of the bottom plate, the top plate is arranged at the top ends of the side plates, a first groove is formed in the inner surface of the bottom plate, a base is arranged in the first groove, a track is arranged on the upper surface of the base, a drainage channel is arranged in the top plate, a drainage groove is arranged on the outer surface of the top plate, filter cotton is arranged in the drainage groove, a drainage channel is arranged in the top plate, the drainage channel is communicated with the lower part of the drainage groove, and the drainage channel is communicated with the lower end of the drainage channel. The problem of earth's surface infiltration can be solved in this tunnel, can also alleviate the inside noise pollution in tunnel, has solved the easy problem that expands of tunnel joint department simultaneously.

摘要：【中文】本发明涉及一种弧面点胶装置及方法，属于机器视觉与精密点胶领域。本发明装置包括机械模块、图像采集模块、图像处理模块、点胶模块和控制模块。本装置使用相机采集圆环形零件图像并通过图像处理算法获取零件的圆心坐标，进行料盘位置补偿；使用相机采集针头图像并手动微调针头使针头图像的圆心与标定好的基线重合，进行针头位置补偿；通过计算机对装置各部分进行联动控制，从而实现圆环形零件的弧面自动点胶。本装置及方法采用控制程序对整个系统进行控制，具备可实现四维联动、全自动和高精度点胶的优点。 【EN】The invention relates to a cambered surface glue dispensing device and method, and belongs to the field of machine vision and precise glue dispensing. The device comprises a mechanical module, an image acquisition module, an image processing module, a dispensing module and a control module. The device uses a camera to collect the images of the circular ring-shaped parts, obtains the coordinates of the circle centers of the parts through an image processing algorithm, and performs position compensation on the material tray; acquiring a needle head image by using a camera and manually finely adjusting the needle head to enable the circle center of the needle head image to be coincident with the calibrated baseline, and performing needle head position compensation; and all parts of the device are subjected to linkage control through a computer, so that automatic dispensing of the cambered surface of the circular ring-shaped part is realized. The device and the method adopt a control program to control the whole system, and have the advantages of realizing four-dimensional linkage, full automation and high-precision dispensing.

摘要：【中文】本发明属于动物细胞基因工程和基因遗传修饰领域，具体涉及一种HER2基因人源化小鼠肿瘤细胞模型的构建方法及其应用，本发明将采用CRISPR技术敲除小鼠HER2基因，同时利用慢病毒感染将人源化HER2基因插入到小鼠乳腺癌细胞4T1中。本发明构建的HER2人源化小鼠乳腺癌人源化肿瘤模型不仅可用于抗体肿瘤药效的评估，对药物开发的临床效果有着重要的指导意义。同时也是抗人HER2抗体药物安全性评价的理想模型，对HER2抗体药物&HER 2‑CAR T、HER 2‑CAR NK免疫细胞疗法的毒理学进行临床前体内评价，填补了市场空白。 【EN】The invention belongs to the field of animal cell genetic engineering and genetic modification, and particularly relates to a construction method and application of a HER2 gene humanized mouse tumor cell model. The HER2 humanized mouse breast cancer humanized tumor model constructed by the invention can be used for evaluating the drug effect of antibody tumor and has important guiding significance on the clinical effect of drug development. Meanwhile, the method is an ideal model for evaluating the safety of the anti-human HER2 antibody drug, clinical precursor internal evaluation is carried out on the toxicology of HER2 antibody drug & HER 2-CAR T and HER 2-CAR NK immune cell therapy, and the market gap is filled.

摘要：【中文】本发明属于小鼠细胞基因工程和基因遗传修饰领域，具体涉及一种EpCAM基因人源化小鼠肿瘤细胞模型的构建方法及其应用，本发明将采用CRISPR技术敲除小鼠EpCAM基因，同时利用慢病毒感染将人源化EpCAM基因插入到小鼠结肠癌细胞CT26中。本发明构建的EpCAM人源化小鼠结肠癌肿瘤模型不仅可用于抗体肿瘤药效的评估，对药物开发的临床效果有着重要的指导意义。同时也是抗人EpCAM抗体药物安全性评价的理想小鼠肿瘤细胞模型，对EpCAM抗体药物&EpCAM‑CAR T、EpCAM‑CAR NK免疫细胞疗法的毒理学进行临床前体内评价，填补了市场空白。 【EN】The invention belongs to the field of mouse cell genetic engineering and genetic modification, and particularly relates to a construction method and application of an EpCAM gene humanized mouse tumor cell model. The EpCAM humanized mouse colon cancer tumor model constructed by the invention can be used for evaluating the drug effect of antibody tumor and has important guiding significance on the clinical effect of drug development. Meanwhile, the model is an ideal mouse tumor cell model for evaluating the safety of anti-human EpCAM antibody drugs, and clinical precursor internal evaluation is performed on the toxicology of EpCAM antibody drugs & EpCAM-CAR T and EpCAM-CAR NK immune cell therapy, so that the market blank is filled.

摘要：【中文】本发明涉及一种CD52基因被敲除的工程化免疫细胞的制备及其用途；特别地，本发明筛选了一种sgRNA分子，可以高特异性地靶向CD52基因，从而提高基因编辑的效率，有利于制备通用型CAR细胞。 【EN】The invention relates to a preparation method and application of an engineered immune cell with a knocked-out CD52 gene; particularly, the sgRNA molecule is screened, the CD52 gene can be targeted at with high specificity, so that the gene editing efficiency is improved, and the preparation of a universal CAR cell is facilitated.

摘要：【中文】本发明属于特丁基对苯二酚含量检测技术领域，公开了一种豆瓣酱中特丁基对苯二酚含量的检测方法，采用超高效液相色谱方法，以甲醇为豆瓣酱中特丁基对苯二酚的提取溶剂，HLB(hydrophile‑lipophile balance)固相萃取小柱净化；十八烷基硅烷键合硅胶为填充剂的色谱柱分离，水和甲醇混合溶剂为流动相，荧光检测器的激发检测波长为293±2nm，发射波长为332±2nm。本发明的方法操作简单可靠，可有效消除杂质干扰，有机溶剂消耗少，重现性好，获得良好的检测效果。 【EN】The invention belongs to the technical field of tert-butyl hydroquinone content detection, and discloses a method for detecting the tert-butyl hydroquinone content in thick broad-bean sauce, which adopts an ultra-high performance liquid chromatography method, takes methanol as an extraction solvent of the tert-butyl hydroquinone in the thick broad-bean sauce, and adopts HLB (hydrophile-lipophile balance) solid phase extraction small column purification; chromatographic column separation with octadecylsilane chemically bonded silica as filler, mixed solvent of water and methanol as mobile phase, excitation detection wavelength of fluorescence detector 293 + -2 nm, and emission wavelength 332 + -2 nm. The method of the invention has simple and reliable operation, can effectively eliminate impurity interference, has less organic solvent consumption and good reproducibility, and obtains good detection effect.

摘要：【中文】本发明具体涉及一种利用正电子湮没技术检测介电材料绝缘性能的方法，现有介电材料的绝缘性能检测方法存在试验装置庞大，耗费成本高以及现有方法只能得到材料表层陷阱信息、不能得到体态缺陷的问题。该方法如下：1、加工待测样品；2、形成检测组件；3、将检测组件固定放置在正电子寿命谱测量仪的两个探测器中间；4、正电子寿命谱测量仪收集到的正电子湮没事件数到达设定数量时，该待测样品测量完毕；5、正电子寿命谱测量仪分析得到正电子在介电材料中湮没的寿命数据，并计算得到正电子在介电材料中湮没的平均寿命；6、获取介电材料的缺陷信息；7、获取介电材料的绝缘性能。 【EN】The invention particularly relates to a method for detecting the insulating property of a dielectric material by using a positron annihilation technology, and the existing method for detecting the insulating property of the dielectric material has the problems of huge test devices and high cost consumption, and the existing method can only obtain the trap information on the surface layer of the material and can not obtain the body state defect. The method comprises the following steps: 1. processing a sample to be detected; 2. forming a detection assembly; 3. fixedly placing the detection assembly between two detectors of a positron life spectrum measuring instrument; 4. when the number of positron annihilation events collected by the positron life spectrum measuring instrument reaches a set number, the measurement of the sample to be measured is finished; 5. analyzing by a positron lifetime spectrum measuring instrument to obtain lifetime data of annihilation of positrons in the dielectric material, and calculating to obtain the average lifetime of annihilation of the positrons in the dielectric material; 6. acquiring defect information of the dielectric material; 7. the insulating property of the dielectric material is obtained.