摘要：【中文】本发明属于荧光检测技术领域，具体公开了一种以鲁米诺‑铝为荧光纳米材料检测GSH的方法。本发明利用聚集诱导荧光原理，以铝离子作为配位中心离子将鲁米诺聚集在一起形成鲁米诺‑铝荧光纳米材料(luminol‑Al NPs)。基于荧光共振能量转移(FRET)原理及MnO₂纳米片与谷胱甘肽的氧化还原反应，luminol‑Al NPs可以用于检测谷胱甘肽，其检测范围从0.2～20μM，最低检测限为45nM，其他金属离子和生物小分子对谷胱甘肽的检测无干扰，本方法用于检测谷胱甘肽具有较高的灵敏度、选择性和准确性。 【EN】The invention belongs to the technical field of fluorescence detection, and particularly discloses a method for detecting GSH by using luminol-aluminum as a fluorescent nano material. The invention utilizes the aggregation-induced fluorescence principle, and uses aluminum ions as coordination center ions to aggregate luminol together to form luminol-aluminum fluorescent nano materials (luminol-Al NPs). Based on Fluorescence Resonance Energy Transfer (FRET) principle and MnO₂The oxidation-reduction reaction of the nanosheets and glutathione, the luminol-Al NPs can be used for detecting the glutathione, the detection range is 0.2-20 mu M, the minimum detection limit is 45nM, other metal ions and small biological molecules have no interference on the detection of the glutathione, and the method has high sensitivity, selectivity and accuracy when used for detecting the glutathione.

摘要：【中文】本发明属于荧光检测技术领域，具体公开了一种以戊二醛‑壳聚糖非共轭荧光聚合物为荧光探针检测GSH的方法，所述方法以戊二醛‑壳聚糖非共轭荧光聚合物(GCPF)为荧光探针，以MnO₂纳米片为荧光淬灭剂来检测谷胱甘肽(GSH)。本发明可用于定量测定GSH，线性范围为0.5～50μM，最低检测限为(LOD)为84nM，其他离子和生物分子对GSH的检测无干扰，选择性高。将本方法用于检测血清样品中的GSH，其回收率为101.48～103.23％，在生物分析中的具有较好的准确性。 【EN】The invention belongs to the technical field of fluorescence detection, and particularly discloses a method for detecting GSH by taking glutaraldehyde-chitosan non-conjugated fluorescent polymer as a fluorescent probe₂The nano-sheet is a fluorescence quenching agent for detecting Glutathione (GSH). The method can be used for quantitatively determining the GSH, the linear range is 0.5-50 mu M, the lowest limit of detection (LOD) is 84nM, other ions and biomolecules have no interference to the GSH detection, and the selectivity is high. The method is used for detecting the GSH in the serum sample, the recovery rate is 101.48-103.23%, and the method has better accuracy in biological analysis.

摘要：【中文】本发明涉及一种向日葵加工用脱粒机，包括机架、机架两侧设置的一号电机和二号电机及机架上端部设置于进料槽；机架上设置有两组对称分布的转轴，转轴上下为一组，且两组转轴上分别配合连接有传动带，该传动带带面上设置有均匀分布的刺钉；转轴下方对应机架上依次倾斜设置有上下分布的第一振动筛和第二振动筛，该第二振动筛顶端对应机架上固定有鼓风机；一号电机和二号电机分别通过皮带与相应转轴传动连接，且二者转向相反，转速不同。本发明脱粒效果好，脱粒效率高，不会损伤葵花籽。 【EN】The invention relates to a threshing machine for processing sunflowers, which comprises a frame, a first motor and a second motor which are arranged on two sides of the frame, and a feeding chute arranged at the upper end part of the frame; two groups of symmetrically distributed rotating shafts are arranged on the rack, the upper part and the lower part of each rotating shaft are provided with one group, the two groups of rotating shafts are respectively matched and connected with a driving belt, and uniformly distributed barbed nails are arranged on the surface of the driving belt; a first vibrating screen and a second vibrating screen which are distributed up and down are sequentially arranged below the rotating shaft and on the corresponding rack in an inclined mode, and an air blower is fixed at the top end of the second vibrating screen on the corresponding rack; the first motor and the second motor are in transmission connection with corresponding rotating shafts through belts respectively, and the rotating directions of the first motor and the second motor are opposite, and the rotating speeds are different. The invention has good threshing effect and high threshing efficiency, and can not damage sunflower seeds.

摘要：【中文】本发明公开了一种用于检测体液中戊型肝炎病毒的引物组合，其包括逆转录引物组、用于检测348bp片段引物组、用于检测B片段引物组；该方法旨在将微量的RNA通过逆转录预扩增得到较高浓度的DNA模板，结合琼脂糖凝胶电泳法检测样本中348bp片段与B片段情况；可检测样本RNA浓度低至100拷贝，且可以同时检测HEV基因348bp与B片段，本发明提供的检测方法灵敏度高，特异性强，操作简便，检测时间短，适用于大规模样品的快速检测，为传染源调查、传播环境、病毒来源工作奠定基础。 【EN】The invention discloses a primer combination for detecting hepatitis E virus in body fluid, which comprises a reverse transcription primer group, a primer group for detecting 348bp fragments and a primer group for detecting B fragments; the method aims to obtain a DNA template with higher concentration by carrying out reverse transcription pre-amplification on trace RNA, and detecting the conditions of a 348bp fragment and a B fragment in a sample by combining an agarose gel electrophoresis method; the detection method provided by the invention has the advantages of high sensitivity, strong specificity, simple and convenient operation and short detection time, is suitable for rapid detection of large-scale samples, and lays a foundation for infection source investigation, propagation environment and virus source work.

摘要：【中文】本发明公开了一种基于互联网的电梯安全监控系统，用于解决现有的电梯不能提前获取等待乘坐电梯人员的到达的楼层号以及等待乘坐电梯人员较多时，乘坐混乱，导致拥挤，乘坐不合理的问题；包括电梯模块、服务器、注册登录模块、统计计算模块和删选模块；所述电梯模块包括电梯监控单元、人脸识别单元、分析单元、播报显示单元、处理器、执行单元、通信单元、存储单元和管理单元；本发明通过对电梯安全进行安全监控，提高用户乘坐电梯的安全性；对人脸识别获取到对应的预设楼层号，用户可以不用按电梯按钮，提高使用的方便；对等待乘坐电梯人员进行排序值计算，便于用户合理乘坐；对用户的删选值计算，节省存储单元的存储空间。 【EN】The invention discloses an elevator safety monitoring system based on the internet, which is used for solving the problems that the existing elevator cannot acquire the number of a floor waiting for the arrival of an elevator passenger in advance and the elevator passenger is in a more number, the elevator passenger is in a disordered state, the elevator passenger is crowded and the elevator passenger is unreasonable; the system comprises an elevator module, a server, a registration login module, a statistical calculation module and a deletion module; the elevator module comprises an elevator monitoring unit, a face recognition unit, an analysis unit, a broadcast display unit, a processor, an execution unit, a communication unit, a storage unit and a management unit; the safety of the elevator is monitored safely, so that the safety of a user taking the elevator is improved; the corresponding preset floor number is acquired by face identification, so that a user does not need to press an elevator button, and the use convenience is improved; the sequencing value of the elevator waiting to be taken is calculated, so that a user can conveniently and reasonably take the elevator; and the deletion value of the user is calculated, so that the storage space of the storage unit is saved.

摘要：【中文】本发明属于基因治疗领域，更具体地，涉及一组肝脏特异性启动子及其应用。本发明公开了一组调控基因在肝脏系统特异性高表达的小尺寸的重组核酸序列。所述重组调控序列片段与目前所报道的相近尺寸大小的其他序列相比，驱动报告基因和人凝血因子FVIII在肝脏系统中表达能力明显增强，适用于重组腺病毒伴随病毒(rAAV)介导的基因治疗。 【EN】The invention belongs to the field of gene therapy, and particularly relates to a group of liver-specific promoters and application thereof. The invention discloses a group of small-sized recombinant nucleic acid sequences for regulating and controlling the specificity and high expression of genes in a liver system. Compared with other sequences with similar sizes reported at present, the recombinant regulatory sequence fragment obviously enhances the expression capability of a driving reporter gene and human coagulation factor FVIII in a liver system, and is suitable for recombinant adenovirus associated virus (rAAV) mediated gene therapy.

摘要：【中文】本发明涉及一种超大现浇重力式锚碇及施工方法，包括：大现浇重力式锚碇悬臂模板体系以及超大重力式锚碇温控系统；现浇重力式锚碇为钢筋砼结构，其结构主要由散索鞍支墩基础、锚块、散索鞍支墩、前锚室和压重块组成。本发明的有益效果是：本发明中支架、模板及施工荷载全部由预埋件承担，不需另搭脚手架，适于高空作业；各组件为拼装式连接，连接方式灵活可靠，组装方便；各连接件标准化程度高，通用性强；本发明中模板部分可相对支撑架部分上下左右调节，悬臂支架设有斜撑，可方便调整模板的垂直度，最大角度为±18°，使用灵活。 【EN】The invention relates to an oversized cast-in-situ gravity type anchorage and a construction method, which comprises the following steps: a large cast-in-situ gravity type anchorage cantilever template system and an ultra-large gravity type anchorage temperature control system; the cast-in-situ gravity anchor is a reinforced concrete structure and mainly comprises a cable saddle buttress foundation, an anchor block, a cable saddle buttress, a front anchor chamber and a ballast block. The invention has the beneficial effects that: in the invention, the bracket, the template and the construction load are all borne by the embedded parts, and no additional scaffold is needed, so that the method is suitable for high-altitude operation; all components are connected in an assembling mode, the connection mode is flexible and reliable, and the assembly is convenient; each connecting piece has high standardization degree and strong universality; the template part can be adjusted up and down, left and right relative to the support frame part, the cantilever support is provided with the inclined strut, the perpendicularity of the template can be conveniently adjusted, the maximum angle is +/-18 degrees, and the use is flexible.

摘要：【中文】本发明涉及材料制备技术领域，提供了一种单相Cu₂BaSnS₄薄膜的制备方法。本发明通过将铜盐、锡盐、硫脲和钡盐溶解于乙二醇甲醚中制备前驱溶液，然后再将此前驱溶液旋涂成膜，再采用退火和硫化处理，最终得到单相Cu₂BaSnS₄薄膜。本发明提供的方法无需使用磁控溅射设备，工艺简单，使用设备易得，而且制备得到的单相Cu₂BaSnS₄薄膜纯度较高，对于Cu₂BaSnS₄薄膜大规模廉价制备提供坚实基础。 【EN】The invention relates to the technical field of material preparation, and provides single-phase Cu₂BaSnS₄A method for preparing a film. The method comprises the steps of dissolving copper salt, tin salt, thiourea and barium salt in ethylene glycol monomethyl ether to prepare a precursor solution, then spin-coating the precursor solution to form a film, and finally obtaining the single-phase Cu by annealing and vulcanizing₂BaSnS₄A film. The method provided by the invention does not need magnetron sputtering equipment, has simple process and easily obtained equipment, and the prepared single-phase Cu₂BaSnS₄The film purity is higher for Cu₂BaSnS₄The large-scale cheap preparation of the film provides a solid foundation.

摘要：【中文】本发明公开了一种基于物联网的电梯曳引机钢丝绳状态预警系统，包括预警评估系统与警报终端，所述预警评估系统包括第一钢丝绳检测模块、第二钢丝绳检测模块、第三钢丝绳检测模块、数据接收模块、数据处理模块、预警评级模块、总控模块、预警发送模块与警报终端；所述第一钢丝绳检测模块用于通过磁检测法检测钢丝绳状态，并获取到第一检测数据，所述第二钢丝绳检测模块用于通过电流法检测钢丝绳状态，并获取到第二检测数据，所述第三钢丝绳检测模块用于通过强磁检测法检测钢丝绳状态，并获取到第三检测数据；本发明的有益效果是：能够有效提升该系统的安全性，让使用者能够更加直观的发现生成预警的信息。 【EN】The invention discloses an elevator traction machine steel wire rope state early warning system based on the Internet of things, which comprises an early warning evaluation system and an alarm terminal, wherein the early warning evaluation system comprises a first steel wire rope detection module, a second steel wire rope detection module, a third steel wire rope detection module, a data receiving module, a data processing module, an early warning rating module, a master control module, an early warning sending module and an alarm terminal; the first steel wire rope detection module is used for detecting the state of a steel wire rope by a magnetic detection method and acquiring first detection data, the second steel wire rope detection module is used for detecting the state of the steel wire rope by a current method and acquiring second detection data, and the third steel wire rope detection module is used for detecting the state of the steel wire rope by a strong magnetic detection method and acquiring third detection data; the invention has the beneficial effects that: the safety of the system can be effectively improved, and users can more intuitively find and generate early warning information.

摘要：【中文】本发明公开了一种基于语音助手的电梯选层控制系统及方法，利用对语音助手的改造让各种智能设备和非智能电梯结合在一起，有效降低了语音控制电梯的门槛，实现了智能控制选层的目的，提升电梯的智能水平。针对现有语音识别的识别率容易收到干扰，存在无法识别的问题，利用影像采集模块、面部识别模块、声纹识别模块、记录生成模块以及补偿模块相互配合，通过对面部特征、动作特征、选层信息进行对比，提取出选层信息，能够有效解决在语音识别失败后，需要用户重复操作的问题，进一步提高了识别效率和智能化。 【EN】The invention discloses a floor selection control system and method of an elevator based on a voice assistant, which combines various intelligent devices with a non-intelligent elevator by utilizing the improvement of the voice assistant, effectively reduces the threshold of the voice control elevator, realizes the purpose of intelligently controlling the floor selection and improves the intelligent level of the elevator. The problem that the existing voice recognition is easy to interfere and cannot be recognized is solved, the image acquisition module, the face recognition module, the voiceprint recognition module, the record generation module and the compensation module are matched with one another, and layer selection information is extracted by comparing face features, action features and layer selection information, so that the problem that a user needs to operate repeatedly after voice recognition fails can be effectively solved, and the recognition efficiency and the intellectualization are further improved.