摘要：【中文】本发明公开了一种基于卷积神经网络的图书采购推荐方法及装置，首先，获取历史书单数据和历史订单数据；然后将历史书单数据与历史订单数据进行匹配，根据匹配结果将历史书单数据中的图书划分为购买和未购买两个类别；接着从带有标签的历史书单数据划分出训练集；接下来将训练集中包含的信息转化为向量的表示形式；再利用训练集对应的向量对预先构建的神经网络模型进行训练；最后将待处理图书的信息转换为向量后，传入训练好的神经网络模型，得到待处理图书的采购推荐结果。本发明的方法可以利用构建的神经网络模型对图书进行购买推荐，并实现了推荐效率以及推荐准确性的提高。 【EN】The invention discloses a book purchasing recommendation method and device based on a convolutional neural network, and the method comprises the following steps of firstly, acquiring history book order data and historical order data; then matching the historical book order data with the historical order data, and dividing the books in the historical book order data into two categories of purchase and unpurchased according to the matching result; then, marking out a training set from the data of the history list with the label; then converting the information contained in the training set into a vector representation form; then, training a pre-constructed neural network model by using vectors corresponding to the training set; and finally, converting the information of the book to be processed into vectors, and transmitting the vectors into the trained neural network model to obtain a purchasing recommendation result of the book to be processed. The method can utilize the built neural network model to purchase and recommend the book, and improves the recommendation efficiency and the recommendation accuracy.

摘要：【中文】本说明书提供商品结算方法、服务器、电子设备、介质和终端。所述方法可以包括：接收结算终端提供的图像和重量；对所述图像进行识别，得出所述图像所表示的商品的商品信息；根据所述商品信息和所述重量生成结算信息；将所述结算信息发送给所述结算终端，以用于所述结算终端进行结算。给消费者带来了便利。 【EN】The present specification provides a commodity settlement method, a server, an electronic device, a medium, and a terminal. The method may include: receiving an image and a weight provided by a settlement terminal; identifying the image to obtain commodity information of a commodity represented by the image; generating settlement information according to the commodity information and the weight; and sending the settlement information to the settlement terminal for settlement by the settlement terminal. Bringing convenience to consumers.

摘要：【中文】本发明提供了可用于在工业相关条件下不对称合成β‑羟基‑α‑氨基酸的工程化多肽，这些工程化多肽是以催化合成(2S,3R)‑2‑氨基‑3‑羟基‑3‑(4‑硝基苯)丙酸的能力为指标，进过定向进化过程开发而得到的。本公开内容还提供了编码工程化多肽的多核苷酸、能够表达工程化多肽的宿主细胞和使用工程化多肽产生β‑羟基‑α‑氨基酸的方法。与其他制备方法相比，使用本发明的工程化多肽用于β‑羟基‑α‑氨基酸的制备，所得产物中立体异构体纯度高，反应条件温和，污染小，能耗低，因此具有很好的工业应用前景。 【EN】The invention provides engineered polypeptides for asymmetrically synthesizing β -hydroxy- α -amino acid under industrially relevant conditions, which are obtained by developing a directed evolution process with the ability of catalytically synthesizing (2S,3R) -2-amino-3-hydroxy-3- (4-nitrophenyl) propionic acid as an index, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides and a method for producing β -hydroxy- α -amino acid by using the engineered polypeptides.

摘要：【中文】本发明公开了一种大肠杆菌诱导蛋白表达的发酵方法，属于生物技术领域。该发酵方法包括以下步骤：将待发酵的种子液接种于发酵培养液中，并置于0.05~0.1MPa的压力条件下进行恒温发酵培养6~10h后，得到发酵底液；往上述发酵底液中添加诱导剂；所述诱导剂在所述发酵底液中质量浓度为0.1~1.5mmol/L；往上述加有诱导剂的发酵底液中添加补料液，并继续进行恒温发酵培养20~48h，得到发酵产物；所述补料液与所述加有诱导剂的发酵底液的体积比为(0.5~2):1。本发明提供的发酵方法可以在36~40℃的环境下进行诱导蛋白的表达，其无需对发酵底液进行快速降温，也可以达到与降温到25~30℃的蛋白表达量，从而可以大大降低能耗以及节约生产成本。 【EN】The invention discloses a fermentation method for expressing escherichia coli induced protein, and belongs to the technical field of biology. The fermentation method comprises the following steps: inoculating a seed solution to be fermented into a fermentation culture solution, and performing constant-temperature fermentation culture for 6-10 h under the pressure condition of 0.05-0.1 MPa to obtain a fermentation base solution; adding an inducer to the fermentation base solution; the mass concentration of the inducer in the fermentation base solution is 0.1-1.5 mmol/L; adding a feed liquid into the fermentation base liquid added with the inducer, and continuously carrying out constant-temperature fermentation culture for 20-48 h to obtain a fermentation product; the volume ratio of the feed liquid to the fermentation base liquid added with the inducer is (0.5-2): 1. The fermentation method provided by the invention can be used for inducing protein expression in an environment of 36-40 ℃, does not need to rapidly cool the fermentation base liquid, and can reach the protein expression amount of cooling to 25-30 ℃, thereby greatly reducing energy consumption and saving production cost.

摘要：【中文】本发明提供一种工业用酶液的热处理澄清方法，包括以下步骤：(1)选择目的蛋白培养发酵：选择目的蛋白，按照现有发酵生产工艺发酵得到发酵液，再将发酵液进行细胞破碎处理，即得细胞破碎液；(2)热处理澄清：将步骤(1)所得细胞破碎液进行热处理澄清；(3)后处理：将步骤(2)热处理澄清后的液体进行离心处理，并收集清液，即为目的蛋白的工业用酶澄清液。该方法具有工艺简单、成本较低且具有环保优势，澄清后酶液中目的蛋白的酶活损失不大于10％，且浊度低于500NTU并能够通过0.2μm的过滤膜。 【EN】The invention provides a heat treatment clarification method of industrial enzyme liquid, which comprises the following steps: (1) selecting target protein for culture fermentation: selecting target protein, fermenting according to the existing fermentation production process to obtain fermentation liquor, and then performing cell disruption treatment on the fermentation liquor to obtain cell disruption liquid; (2) and (3) heat treatment clarification: carrying out heat treatment clarification on the cell disruption solution obtained in the step (1); (3) and (3) post-treatment: and (3) centrifuging the liquid subjected to heat treatment and clarification in the step (2), and collecting clear liquid, namely the industrial enzyme clarified liquid of the target protein. The method has the advantages of simple process, low cost and environmental protection, the enzyme activity loss of the target protein in the clarified enzyme solution is not more than 10 percent, the turbidity is lower than 500NTU, and the clarified enzyme solution can pass through a filtering membrane of 0.2 mu m.

摘要：【中文】本发明提供了可用于在工业相关条件下不对称合成β‑羟基‑α‑氨基酸的工程化醛缩酶多肽。本公开内容还提供了编码工程化醛缩酶多肽的多核苷酸、能够表达工程化醛缩酶多肽的宿主细胞和使用工程化醛缩酶多肽产生β‑羟基‑α‑氨基酸的方法。与其他制备方法相比，使用本发明的工程化醛缩酶多肽用于β‑羟基‑α‑氨基酸的制备，所得产物中立体异构体纯度高，反应条件温和，具有很好的工业应用前景。 【EN】The present disclosure also provides polynucleotides encoding the engineered aldolase polypeptides, host cells capable of expressing the engineered aldolase polypeptides, and methods of producing β -hydroxy- α -amino acids using the engineered aldolase polypeptides.

摘要：【中文】本发明涉及医药中间体技术领域，具体涉及一种烯酮还原酶与一种布瓦西坦中间体的制备方法，该制备方法包括以下步骤：在吗啉的催化下，戊醛和乙醛酸反应生成5‑羟基‑4‑正丙基‑2(5H)‑呋喃酮；在硼氢化钠的催化下，5‑羟基‑4‑正丙基‑2(5H)‑呋喃酮脱除羟基生成4‑正丙基‑2(5H)‑呋喃酮；在烯酮还原酶的存在下，4‑正丙基‑2(5H)‑呋喃酮发生还原反应生成目标产物布瓦西坦中间体；该烯酮还原酶的核苷酸序列如SEQ ID NO.1所示，且其氨基酸序列如SEQ ID NO.2所示。本发明所述的布瓦西坦中间体的制备方法仅需3步反应，即可制得布瓦西坦中间体，无需额外化学拆分，整体工艺绿色环保，成本较低，易于实施。 【EN】The invention relates to the technical field of medical intermediates, in particular to a preparation method of ketene reductase and a brivaracetam intermediate, which comprises the following steps: under the catalysis of morpholine, valeraldehyde and glyoxylic acid react to generate 5-hydroxy-4-n-propyl-2 (5H) -furanone; under the catalysis of sodium borohydride, hydroxyl of 5-hydroxyl-4-n-propyl-2 (5H) -furanone is removed to generate 4-n-propyl-2 (5H) -furanone; in the presence of ketene reductase, 4-n-propyl-2 (5H) -furanone is subjected to reduction reaction to generate a target product, namely a brivaracetam intermediate; the nucleotide sequence of the enone reductase is shown as SEQ ID NO.1, and the amino acid sequence thereof is shown as SEQ ID NO. 2. The preparation method of the intermediate of the invention can prepare the intermediate of the invention only by 3 steps of reaction without additional chemical resolution, and has the advantages of green and environment-friendly overall process, low cost and easy implementation.

摘要：【中文】本发明创造公开了吸附式通用测量夹具，包括：夹具座，其设有多个上下设置并且矩阵分布的第一通孔，每个第一通孔内均安装有环形线圈电磁铁；多个负压分管，其与所述第一通孔一一对应设置，每个所述负压分管均包括下软管和具有导磁性的上管体，所述上管体的下端与所述下软管密封连接，每个所述上管体均从下往上穿过对应的环形线圈电磁铁并延伸至所述夹具座的上方；用以提供负压的负压总管，每个所述下软管均与所述负压总管连通，每个所述负压分管均连接有气阀，所述气阀用以控制所述负压分管的通断。与现有技术相比，本发明创造能够对各种不同形状、不同尺寸的工件进行吸附定位，极大地提高了测量夹具的通用性。 【EN】The invention discloses an adsorption type universal measuring clamp, which comprises: the fixture seat is provided with a plurality of first through holes which are arranged up and down and distributed in a matrix manner, and each first through hole is internally provided with an annular coil electromagnet; the negative pressure branch pipes are arranged in one-to-one correspondence with the first through holes, each negative pressure branch pipe comprises a lower hose and an upper pipe body with magnetic conductivity, the lower end of the upper pipe body is connected with the lower hose in a sealing manner, and each upper pipe body penetrates through the corresponding annular coil electromagnet from bottom to top and extends to the upper part of the clamp seat; the negative pressure branch pipes are connected with air valves, and the air valves are used for controlling the on-off of the negative pressure branch pipes. Compared with the prior art, the invention can adsorb and position workpieces with different shapes and sizes, and greatly improves the universality of the measuring clamp.