摘要：【中文】本发明属于果蔬保鲜领域，具体涉及一种葡萄果实防腐烂的保鲜液及其制备方法和保鲜纸，包括以下质量百分比的组分：1.5％壳聚糖、1％生物明胶，0.05％‑0.1％的柠檬酸、1.5％‑3％姜黄素、1.5％‑3％壳寡糖，余量水。本申请的保鲜纸的涂膜基质明胶采用生物来源，成膜性好，可降解无毒性，同时选用壳聚糖一方面有成膜性，同时其对交链孢霉等真菌均有较好的抑制作用，以生物明胶和壳聚糖为成膜材料载体，添加生物来源的保鲜液成分涂布于纸上，有效成分为壳聚糖、壳寡糖和柠檬酸及姜黄素的混合物，持效期长，并且无化学污染，对葡萄果实采后腐烂有明显抑制效果。 【EN】The invention belongs to the field of fruit and vegetable preservation, and particularly relates to a grape fruit rot-proof preservative solution, a preparation method thereof and preservative paper, which comprise the following components in percentage by mass: 1.5 percent of chitosan, 1 percent of biological gelatin, 0.05 percent to 0.1 percent of citric acid, 1.5 percent to 3 percent of curcumin, 1.5 percent to 3 percent of chitosan oligosaccharide and the balance of water. The coating matrix gelatin of the preservative paper is biologically derived, has good film forming property, can be degraded and is non-toxic, chitosan is selected to have film forming property on one hand and has good inhibition effect on fungi such as alternaria, biological gelatin and chitosan are used as film forming material carriers, preservative solution components of biological sources are added and coated on the paper, the effective components are the mixture of chitosan, chitosan oligosaccharide, citric acid and curcumin, the effect duration is long, no chemical pollution is caused, and the preservative paper has obvious inhibition effect on rot of grape fruits after picking.

摘要：【中文】本发明提供了嗜酸乳杆菌的液体发酵菌剂的制备方法，经过一级种子培养、二级种子扩培、高密度发酵培养和后发酵的培养方法得到含有高浓度活菌及代谢产物的嗜酸乳杆菌的液体发酵菌剂。该制备方法对培养基和发酵条件进行了优化、补料工艺及大规模发酵工艺参数优化，大大提高了产品性能与生产效率，代谢产物富含量显著高于现有发酵水平。该制备过程无需菌体浓缩、保护剂或载体混合、冷冻干燥、粉碎等过程，其生产工艺相对简单，过程成本低，适合大规模工业化生产。此外，本发明提供的嗜酸乳杆菌的液体发酵菌剂通过添加至饮水与饲料中应用于家禽养殖，在家禽饮水中投放极为方便、不会产生堵塞饮水管道的问题，具有较大的应用前景与市场空间。 【EN】The invention provides a preparation method of a liquid fermentation microbial inoculum of lactobacillus acidophilus, which is used for obtaining the liquid fermentation microbial inoculum of the lactobacillus acidophilus containing high-concentration live bacteria and metabolites through a primary seed culture method, a secondary seed propagation culture method, a high-density fermentation culture method and a post-fermentation culture method. The preparation method optimizes the culture medium and fermentation conditions, optimizes the feeding process and large-scale fermentation process parameters, greatly improves the product performance and production efficiency, and remarkably increases the content of metabolites higher than the existing fermentation level. The preparation process does not need processes of thallus concentration, protective agent or carrier mixing, freeze drying, crushing and the like, has relatively simple production process and low process cost, and is suitable for large-scale industrial production. In addition, the liquid fermentation microbial inoculum of lactobacillus acidophilus provided by the invention is added into drinking water and feed to be applied to poultry breeding, is very convenient to put in the drinking water of the poultry, does not cause the problem of blocking drinking water pipelines, and has a wide application prospect and a wide market space.

摘要：【中文】本发明公开了一种中华绒螯蟹复合发酵饲料及其制备方法，该饲料包括质量比为0.3‑4：60000的菌剂和发酵底物；还可包括中草药复合提取物，其与发酵底物的质量比为0.3‑5：60000。其中，发酵底物按照质量份包含豆粕6‑16份、小麦20‑30份、玉米30‑50份、麸皮5‑25份、糖蜜1‑5份、复合预混料1‑3份和水10‑20份；菌剂包含植物乳杆菌1.6‑2.6份、枯草芽孢杆菌3.0‑3.4份、酿酒酵母菌2.9‑3.6份、布氏乳杆菌2.1‑2.7份、粪肠球菌1.1‑1.7份。本发明保证了肠道益生菌在本产品中的数量、活性和存活时间，特定组成的中草药复合提取物和复合预混料，具有微生态制剂特性及类似抗生素生长因子的功能，可显著增强中华绒螯蟹的免疫力和抗病能力，降低疾病发生率，提高其饲养质量。 【EN】The invention discloses a eriocheir sinensis compound fermented feed and a preparation method thereof, wherein the feed comprises the following components in percentage by mass of 0.3-4: 60000 and fermentation substrate; the fermentation substrate can also comprise Chinese herbal medicine compound extracts, and the mass ratio of the Chinese herbal medicine compound extracts to the fermentation substrate is 0.3-5: 60000. wherein the fermentation substrate comprises 6-16 parts of soybean meal, 20-30 parts of wheat, 30-50 parts of corn, 5-25 parts of bran, 1-5 parts of molasses, 1-3 parts of compound premix and 10-20 parts of water according to the mass parts; the microbial inoculum comprises 1.6-2.6 parts of lactobacillus plantarum, 3.0-3.4 parts of bacillus subtilis, 2.9-3.6 parts of saccharomyces cerevisiae, 2.1-2.7 parts of lactobacillus buchneri and 1.1-1.7 parts of enterococcus faecalis. The invention ensures the quantity, activity and survival time of intestinal probiotics in the product, and the Chinese herbal medicine compound extract and compound premix with specific composition have the characteristics of microecological preparation and the function similar to antibiotic growth factor, can obviously enhance the immunity and disease resistance of the eriocheir sinensis, reduce the disease incidence and improve the feeding quality.

摘要：【中文】本发明一方面提供了一种嗜酸乳杆菌产细菌素，其氨基酸序列如SEQ ID NO.1所示，其分子量为1527Da。本发明另一方面提供了该细菌素的分离纯化方法：步骤一，将该细菌素发酵液依次经过经过陶瓷膜、10kDa超滤膜和1kDa超滤膜过滤过滤处理，对截留液进行真空浓缩，得到细菌素粗提物；步骤二，将细菌素粗提物经过Sephadex G‑25分离收集活性馏分；步骤三，将细菌素活性馏分通过HPLC分离纯化细菌素。本发明提供的嗜酸乳杆菌产细菌素对革兰氏阳性菌和革兰氏阴性菌均具有较好的抑菌效果，且热稳定性好，具有较广谱的抑菌效果和良好的预防腹泻的作用，可在调节动物肠道菌群平衡的应用中发挥重要作用。 【EN】On the one hand, the invention provides a bacteriocin produced by lactobacillus acidophilus, the amino acid sequence of the bacteriocin is shown in SEQ ID No.1, and the molecular weight of the bacteriocin is 1527 Da. The invention also provides a method for separating and purifying the bacteriocin, which comprises the following steps: sequentially filtering and filtering the bacteriocin fermentation liquor by a ceramic membrane, a 10kDa ultrafiltration membrane and a 1kDa ultrafiltration membrane, and concentrating trapped fluid in vacuum to obtain a bacteriocin crude extract; step two, separating and collecting the bacteriocin crude extract by Sephadex G-25 to obtain active fraction; and step three, separating and purifying the bacteriocin by HPLC. The lactobacillus acidophilus bacteriocin provided by the invention has good bacteriostatic effect on gram-positive bacteria and gram-negative bacteria, has good thermal stability, has a broad-spectrum bacteriostatic effect and a good diarrhea prevention effect, and can play an important role in the application of regulating the balance of animal intestinal flora.

摘要：【中文】本发明公开了一种发酵饲料防腐剂的制备方法，通过果胶酶、纤维素酶和β‑葡萄糖苷酶对豆粕和苹果渣进行酶解，将豆粕和苹果渣中的果胶降解为果胶寡糖，包括如下步骤：步骤一，以豆粕为原料，加入酶制剂进行酶解；步骤二，以苹果渣为原料，加入酶制剂进行酶解；步骤三，将步骤一得到的豆粕酶解物和步骤二得到的苹果渣酶解物经喷雾干燥后混合即可制得发酵饲料防腐剂。本发明的发酵饲料防腐剂的制备方法操作简单，原料易得，成本低廉，可大规模推广；所制备的发酵饲料防腐剂热稳定性好，抑菌效果显著。 【EN】The invention discloses a preparation method of a fermented feed preservative, which is characterized in that bean pulp and apple pomace are subjected to enzymolysis through pectinase, cellulase and β -glucosidase, and pectin in the bean pulp and the apple pomace is degraded into pectin oligosaccharide.

摘要：【中文】本发明公开了一种固态发酵生产乳酸菌素的方法，包括如下步骤：菌株的的获取、筛选；菌株的三级扩培；固态发酵物料原料的处理；菌种液的调制；接种；发酵；干燥；粉碎和打包。本发明提供的一种固态发酵生产乳酸菌素的方法实现了本来由液态发酵方式生产的产品的所有指标(抑菌能力、总酸含量和蛋白质含量)，通过优化固态发酵生产乳酸菌素的工艺及参数大幅提高生产效率(生产效率提升60‑70倍)，并且大幅降低生产成本(固态发酵生产的吨干燥成本降低为液态发酵生产成本的10％)。此外，本发明提供的方法，成本低廉，并且可以满足动物养殖中的大规模应用，在农牧业的无抗生素养殖中可以起到很大的作用。 【EN】The invention discloses a method for producing lactein by solid state fermentation, which comprises the following steps: obtaining and screening strains; carrying out three-stage propagation on the strains; treating solid fermentation material raw materials; preparing strain liquid; inoculating; fermenting; drying; and (6) crushing and packaging. The method for producing the lactein by solid state fermentation provided by the invention realizes all indexes (bacteriostatic ability, total acid content and protein content) of the product produced by the liquid state fermentation way, greatly improves the production efficiency (improves the production efficiency by 60-70 times) by optimizing the process and parameters for producing the lactein by solid state fermentation, and greatly reduces the production cost (reduces the ton drying cost of the solid state fermentation production to 10 percent of the production cost of the liquid state fermentation). In addition, the method provided by the invention has low cost, can meet the requirement of large-scale application in animal breeding, and can play a great role in antibiotic-free breeding in farming and animal husbandry.

摘要：【中文】本申请涉及一种含纳米粉体的天然酯绝缘油的制备方法及制备装置，所述制备方法包括如下步骤：将微米氮化硼粉末和天然酯绝缘油的混合液通过间歇式超声模式进行超声剥离，之后将超声剥离后的混合液离心，收集含二维纳米氮化硼的上清液，所述二维纳米氮化硼的厚度为10nm以下，宽度为500nm以下；将含二维纳米氮化硼的上清液与天然酯绝缘油在真空环境下进行充分混合，之后对混合溶液进行脱气脱水，得到含纳米粉体的天然酯绝缘油；本发明还提供了一种包括超声模块、离心模块、分析检测模块、真空混合模块和脱气脱水模块的含纳米粉体的天然酯绝缘油的制备装置。本发明制备的绝缘油的导热系数高、介电损耗小、击穿电压高。 【EN】The application relates to a preparation method and a preparation device of natural ester insulating oil containing nano powder, wherein the preparation method comprises the following steps: ultrasonically stripping a mixed solution of micron boron nitride powder and natural ester insulating oil in an intermittent ultrasonic mode, centrifuging the ultrasonically stripped mixed solution, and collecting a supernatant containing two-dimensional nano boron nitride, wherein the thickness of the two-dimensional nano boron nitride is less than 10nm, and the width of the two-dimensional nano boron nitride is less than 500 nm; fully mixing the supernatant containing the two-dimensional nano boron nitride with the natural ester insulating oil in a vacuum environment, and then degassing and dehydrating the mixed solution to obtain the natural ester insulating oil containing the nano powder; the invention also provides a preparation device of the natural ester insulating oil containing the nano powder, which comprises an ultrasonic module, a centrifugal module, an analysis and detection module, a vacuum mixing module and a degassing and dehydration module. The insulating oil prepared by the invention has the advantages of high heat conductivity coefficient, small dielectric loss and high breakdown voltage.