摘要：【中文】本发明提供了一种从江香猪IFN‑λ1基因的检测方法。本发明属于核酸检测领域，特别是一种快速检测目的基因的方法。提供一种从江香猪IFN‑λ1基因扩增方法，旨在为后续研究IFN‑λ1的抗病毒活性以及抗病毒类药物研发奠定工作基础。本发明采用淋巴细胞分离法分离淋巴细胞，并提取细胞总RNA，通过RT‑PCR扩增出目的基因，克隆、测序完成以后对IFN‑λ1基因进行相关遗传进化分析，以探寻IFN‑λ1基因与其他哺乳动物之间的相关性，为进一步研究IFN‑λ1基因的抗病毒活性提供依据。 【EN】The invention provides a detection method of a Congjiang fragrant pig IFN-lambda 1 gene. The invention belongs to the field of nucleic acid detection, and particularly relates to a method for rapidly detecting a target gene. Provides a method for amplifying IFN-lambda 1 gene of Jiangxiang pigs, and aims to lay a work foundation for the subsequent research on the antiviral activity of IFN-lambda 1 and the research and development of antiviral drugs. The invention adopts a lymphocyte separation method to separate lymphocytes, extracts total RNA of the cells, amplifies a target gene by RT-PCR, clones and sequences, and then carries out related genetic evolution analysis on the IFN-lambda 1 gene so as to search the correlation between the IFN-lambda 1 gene and other mammals, thereby providing a basis for further researching the antiviral activity of the IFN-lambda 1 gene.

摘要：【中文】本发明公开了一种用于检测PRRSV‑N基因的特异性引物和探针及其试剂盒与使用方法。本发明发现了适合作检测PRRSV‑N基因的特异性引物和探针，并采用基因克隆技术，将PRRSV‑N基因片段插入到载体pMD18‑T中，获得含有PRRSV‑N基因片段的重组质粒，以此作为标准品，并组合得到PCR检测试剂盒，通过优化PCR反应条件，建立以实时荧光定量聚合酶链反应为平台的检测方法，并对所建立的方法进行评估。能定性或定量检测或辅助检测PRRSV‑N基因；制备定性或定量检测或辅助检测PRRSV‑N基因产品。对判PRRSV‑N基因的含量以至于后续研究具有重要意义。 【EN】The invention discloses a specific primer and a probe for detecting PRRSV-N gene, a kit and a use method thereof. The invention finds out specific primers and probes suitable for detecting PRRSV-N genes, adopts a gene cloning technology to insert PRRSV-N gene fragments into a vector pMD18-T to obtain recombinant plasmids containing the PRRSV-N gene fragments, takes the recombinant plasmids as a standard substance and combines the recombinant plasmids to obtain a PCR detection kit, establishes a detection method taking real-time fluorescent quantitative polymerase chain reaction as a platform by optimizing PCR reaction conditions, and evaluates the established method. Can qualitatively or quantitatively detect or assist in detecting the PRRSV-N gene; preparing a product for qualitatively or quantitatively detecting or assisting in detecting the PRRSV-N gene. Has important significance for judging the content of PRRSV-N gene so as to follow-up research.

摘要：【中文】本发明公开了一种面向大规模光伏发电系统的信息物理建模方法，所述方法包括：建立IO接口；通过IO接口融合光伏发电系统物理系统模型和光伏发电系统数据传输模型。本发明通过网络接口实现光伏发电系统物理系统模型和光伏发电系统数据传输模型的仿真交互，由于物理仿真和信息仿真分别采用各自的专业软件建立，该系统具备可拓展、可兼容、可替换等开发灵活特性，可提高大规模光伏发电系统CPS仿真效率和精度，能对大电网信息物理系统的建模、计算和控制提供支撑。 【EN】The invention discloses an information physical modeling method for a large-scale photovoltaic power generation system, which comprises the following steps: establishing an IO interface; and fusing a photovoltaic power generation system physical system model and a photovoltaic power generation system data transmission model through an IO interface. According to the invention, the simulation interaction of the photovoltaic power generation system physical system model and the photovoltaic power generation system data transmission model is realized through the network interface, and as the physical simulation and the information simulation are respectively established by adopting respective professional software, the system has the development flexibility characteristics of expandability, compatibility, replaceability and the like, the CPS simulation efficiency and precision of the large-scale photovoltaic power generation system can be improved, and the support can be provided for modeling, calculation and control of the large-power grid information physical system.

摘要：【中文】本发明公开了一种全景式信息物理融合系统仿真架构及其工作方法，所述系统包括相互连接的软件仿真器和外部装置系统。所述方法包括通过软件仿真器获取物理系统的状态信息数据；根据数据采集协议将所述状态信息数据发送至外部装置系统。本发明通过网络socket接口将软件仿真器和外部装置进行连接，通过UDP协议交互实现CPS仿真装置与外部装置/系统交互，实现信息‑物理仿真交互，系统维护方便，仿真规模可随时增减。 【EN】The invention discloses a panoramic information physical fusion system simulation framework and a working method thereof. The method comprises the steps of acquiring state information data of a physical system through a software simulator; and sending the state information data to an external device system according to a data acquisition protocol. The invention connects the software simulator with the external device through the network socket interface, realizes the interaction between the CPS simulation device and the external device/system through the UDP protocol interaction, realizes the information-physical simulation interaction, is convenient for system maintenance, and can increase and decrease the simulation scale at any time.