摘要：【中文】本发明公开了一种数字PCR芯片，该芯片包括基底层和盖板层，基底层与盖板层之间形成密封腔体，盖板层上设置有至少一个进样孔和至少一个出样孔，基底层上设置有至少一个储液池和至少一个通道，储液池与通道连通，储液池中设置有至少一个微孔。相应的，本发明还公开了一种制备上述数字PCR芯片的方法。本发明芯片集成度高，面积小，通过采用湿法刻蚀工艺和硅‑玻璃键合，可以批量制作高通量的数字PCR芯片；通过流体仿真改进芯片结构，可以防止后续进样出现流动死角；进样过程利用矿物油与样品的膨胀系数差异封锁待测样品于微孔中，均匀独立并有助于防止样品污染，便于制作靶基因测试的高通量、高灵敏度、高精度的数字PCR芯片。 【EN】The invention discloses a digital PCR chip, which comprises a substrate layer and a cover plate layer, wherein a sealed cavity is formed between the substrate layer and the cover plate layer, at least one sample inlet hole and at least one sample outlet hole are arranged on the cover plate layer, at least one liquid storage tank and at least one channel are arranged on the substrate layer, the liquid storage tank is communicated with the channel, and at least one micropore is arranged in the liquid storage tank. Correspondingly, the invention also discloses a method for preparing the digital PCR chip. The chip of the invention has high integration level and small area, and the high-flux digital PCR chip can be manufactured in batch by adopting a wet etching process and silicon-glass bonding; the chip structure is improved through fluid simulation, so that flowing dead corners of subsequent sample introduction can be prevented; in the sample introduction process, the sample to be tested is blocked in the micropores by utilizing the expansion coefficient difference between the mineral oil and the sample, so that the sample is uniform and independent, the sample pollution is prevented, and the high-throughput, high-sensitivity and high-precision digital PCR chip for target gene testing is convenient to manufacture.

摘要：【中文】本发明提供了一种水上拼装首节钢围堰施工工艺，包括以下步骤：(1)在围堰基槽的下游搭设水上拼装平台，水上拼装平台由两个小平台组成，在两个小平台之间搭设两个钢平台用于连通该两个小平台；(2)在两个小平台上各自完成1/3弧段钢围堰的安装；(3)剩余的一个1/6弧段钢围堰吊装在两个1/3弧段钢围堰的一端，并在悬臂情况下完成两个1/3弧段钢围堰和一个1/6弧段钢围堰的拼装，焊接时两者之间用限位板约束并临时焊接固定；(4)剩余的另一个1/6弧段钢围堰吊装在两个1/3弧段钢围堰的另一端，并在悬臂情况下完成两个1/3弧段钢围堰和一个1/6弧段钢围堰的拼装，焊接时两者之间用限位板约束并临时焊接固定。本发明的围堰基槽的爆破开挖与首节钢围堰的水上拼装同步进行，节约了近2个月工期。 【EN】The invention provides a construction process for assembling a first section of steel cofferdam on water, which comprises the following steps: (1) an overwater assembling platform is erected at the downstream of the cofferdam foundation trench, the overwater assembling platform consists of two small platforms, and two steel platforms are erected between the two small platforms and are used for communicating the two small platforms; (2) the installation of 1/3 arc-section steel cofferdams is respectively completed on the two small platforms; (3) the rest 1/6 arc-section steel cofferdams are hoisted at one end of the two 1/3 arc-section steel cofferdams, the assembly of the two 1/3 arc-section steel cofferdams and the 1/6 arc-section steel cofferdams is completed under the condition of a cantilever, and the two cofferdams are restrained by a limiting plate and are temporarily welded and fixed during welding; (4) and the other residual 1/6 arc-section steel cofferdams are hung at the other ends of the two 1/3 arc-section steel cofferdams, the assembly of the two 1/3 arc-section steel cofferdams and one 1/6 arc-section steel cofferdam is completed under the condition of a cantilever, and the two cofferdams are restrained by a limiting plate and are temporarily welded and fixed during welding. The blasting excavation of the cofferdam foundation trench and the water assembly of the first section of steel cofferdam are carried out synchronously, so that the construction period is saved by about 2 months.

摘要：【中文】本申请公开了一种含有气泡消除结构的聚合酶链式反应芯片及液滴乳液的处理方法，包括设置于所述聚合酶链式反应芯片同一面的进样结构100、微流通道200、气泡漂浮结构300和液滴存储结构400。由于气泡漂浮结构300中的气泡漂浮腔302可以用于对液滴乳液进行气泡消除处理，可以减少气泡的引入甚至消除气泡的引入，以便后续扩增反应的顺利进行。 【EN】The application discloses a polymerase chain reaction chip containing a bubble elimination structure and a processing method of a liquid drop emulsion, which comprises a sample introduction structure 100, a micro-flow channel 200, a bubble floating structure 300 and a liquid drop storage structure 400 which are arranged on the same surface of the polymerase chain reaction chip. Since the bubble floating chamber 302 in the bubble floating structure 300 can be used for bubble elimination treatment of the droplet emulsion, the introduction of bubbles can be reduced or even eliminated, so that the subsequent amplification reaction can be smoothly performed.

摘要：【中文】本发明属于农业微生物技术领域，具体涉及一种利用贝莱斯芽孢杆菌发酵提升烟叶品质的方法。具体方法为将贝莱斯芽孢杆菌(Bacillus velezensis)进行菌种活化、发酵制备成生物制剂，然后均匀喷施于烟叶表面进行发酵。经过发酵后，烟叶中淀粉的降解率可达20.2‑36.2％，烟叶中的香味物质增加。感官评吸结果表明，处理烟叶的香气质提升，香气量增加，浓度增加，口感改善，甜润感增加，杂气减少，品质显著提升。 【EN】The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a method for improving tobacco leaf quality by utilizing Bacillus belgii fermentation. The method specifically comprises the steps of activating and fermenting Bacillus velezensis to prepare a biological agent, and then uniformly spraying the biological agent on the surface of the tobacco leaves for fermentation. After fermentation, the degradation rate of starch in the tobacco leaves can reach 20.2-36.2%, and the fragrance substances in the tobacco leaves are increased. Sensory evaluation results show that the aroma quality of the processed tobacco leaves is improved, the aroma amount is increased, the concentration is increased, the taste is improved, the sweet feeling is increased, the miscellaneous gas is reduced, and the quality is obviously improved.

摘要：【中文】本发明公开了一种膀胱肿瘤化疗药物筛选的芯片器官模型，包括表面修饰塑料或玻璃底座和PDMS膜，PDMS膜设置于表面修饰塑料或玻璃底座的顶部，该PDMS膜的一端开设有换液槽出口，另一端开设有换液槽出口；本发明还公开了一种膀胱肿瘤化疗药物筛选的芯片器官模型的制作方法，本发明的有益效果是：本发明通过应用PDMS膜高分子透气疏水膜进一步优化设计微流控芯片，应用特殊的组织培养芯片对个体化病人的离体肿瘤组织进行高通量培养，在临床中通过其对不同化疗药物反应快速准确筛选化疗药物，提高药物治疗的有效率，降低患者治疗周期；本发明将应用微流控芯片技术应用于膀胱癌个体化治疗中，着眼点在个体化化疗药物的筛选，有助于提高当前临床用药的指导性。 【EN】The invention discloses a chip organ model for screening chemotherapy drugs for bladder tumors, which comprises a surface-modified plastic or glass base and a PDMS membrane, wherein the PDMS membrane is arranged at the top of the surface-modified plastic or glass base, one end of the PDMS membrane is provided with a liquid changing groove outlet, and the other end of the PDMS membrane is provided with a liquid changing groove outlet; the invention also discloses a manufacturing method of the chip organ model for screening the bladder tumor chemotherapy drugs, and the invention has the beneficial effects that: according to the invention, the micro-fluidic chip is further optimally designed by applying the PDMS membrane high-molecular breathable hydrophobic membrane, the special tissue culture chip is applied to carry out high-flux culture on the isolated tumor tissue of the individualized patient, and the chemotherapeutic drugs are rapidly and accurately screened by reacting with different chemotherapeutic drugs in clinic, so that the effective rate of drug treatment is improved, and the treatment period of the patient is shortened; the invention applies the microfluidic chip technology to the individualized treatment of the bladder cancer, focuses on the screening of individualized chemotherapy drugs and is beneficial to improving the guidance of current clinical medication.