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Kong Mengyao
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[发明]
【中文】一种基于CRISPR/Cas9转变水稻果皮色的基因编辑方法及其应用 【EN】Gene editing method for converting rice peel color based on CRISPR/Cas9 and application thereof
申请号:
201911079333.7
公开号:CN110846314A 主分类号:C12N15/113
申请人:
【中文】南京农业大学【EN】NANJING AGRICULTURAL University
申请日:2019.11.07 公开日:2020.02.28
发明人:
【中文】戴伟民
;
强胜
;
宋小玲
;
孔梦瑶
;
孙茜茜【EN】Dai Weimin
;
Qiang Sheng
;
Song Xiaoling
;
Kong Mengyao
;
Sun Qianqian
摘要:【中文】本发明涉及了一种基于CRISPR/Cas9变换水稻果皮色(白至红、红至白)的基因编辑方法,其包括如下步骤:(1)合成转录因子rc基因的特定gRNA‑spacer;(2)构建CRISPR/Cas‑gRNA‑spacer载体;(3)采用农杆菌转化法转化水稻品种的成熟胚、再生植株。T
0
代种子即可实现水稻品种果皮色的白至红、红至白的相互转变。红米在世界各国长期用作滋补食品,是水稻育种的重要目标之一。经过人工长期驯化的水稻果皮色为白色,抗性弱,而来自栽培稻脱驯化的杂草稻果皮色为红色,抗性强。通过相互转换果皮色,可能培育出抗性强的白\红果皮水稻品种。本技术具有良好的商业化应用前景。 【EN】The invention relates to a gene editing method for transforming the peel color (white to red and red to white) of rice based on CRISPR/Cas9, which comprises the following steps: (1) synthesizing a specific gRNA-spacer of a transcription factor rc gene; (2) constructing a CRISPR/Cas-gRNA-spacer vector; (3) mature embryos and regenerated plants of rice varieties are transformed by an agrobacterium transformation method. T is
0
The mutual transformation of the white to red and red to white of the peel color of the rice variety can be realized by the generation of seeds. Red rice is used as a tonic food in countries of the world for a long time and is one of the important targets of rice breeding. Long-term domesticated riceThe peel color of the weedy rice is white and the resistance is weak, while the peel color of the weedy rice which is removed and domesticated from the cultivated rice is red and the resistance is strong. By mutually converting the color of the peel, the rice variety with the white/red peel and strong resistance can be cultivated. The technology has good commercial application prospect.
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2:
[发明]
【中文】一种抗皱保湿护肤水及其制备方法 【EN】Anti-wrinkle moisturizing skin care lotion and preparation method thereof
申请号:
201911218857.X
公开号:CN110840769A 主分类号:A61K8/49
申请人:
【中文】河北翰林生物科技有限公司【EN】HANLIN BIOTECHNOLOGY CO., LTD.
申请日:2019.12.03 公开日:2020.02.28
发明人:
【中文】胡翠霞
;
苏学军
;
关珊
;
孔梦瑶
;
聂珊
;
杨心源
;
李宛津【EN】Hu Cuixia
;
Su Xuejun
;
Guan Shan
;
Kong Mengyao
;
Nie Shan
;
Yang Xinyuan
;
Li Wanjin
摘要:【中文】本发明涉及日化用品领域,更具体地,本发明提供了一种抗皱保湿护肤水及其制备方法。本发明第一方面提供一种抗皱保湿护肤水,按重量份计,制备原料至少包括1~4份调理剂、8~14份保湿剂、0.2~1份防腐剂、0.05~0.1份pH稳定剂、0.1~0.3份增溶剂、0.02~0.1份增稠剂、0.5~2份抗氧剂、2~7份醇类物质;所述增溶剂选自PEG‑40氢化蓖麻油、PEG‑60氢化蓖麻油、PEG‑80氢化蓖麻油中的一种或几种组合。 【EN】The invention relates to the field of daily chemical articles, and particularly provides anti-wrinkle moisturizing lotion and a preparation method thereof. The invention provides anti-wrinkle moisturizing lotion which comprises, by weight, at least 1-4 parts of a conditioner, 8-14 parts of a humectant, 0.2-1 part of a preservative, 0.05-0.1 part of a pH stabilizer, 0.1-0.3 part of a solubilizer, 0.02-0.1 part of a thickener, 0.5-2 parts of an antioxidant and 2-7 parts of an alcohol substance; the solubilizer is selected from one or more of PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil and PEG-80 hydrogenated castor oil.
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