摘要：【中文】本发明属于生物医药技术领域，公开了一种靶向治疗习惯性流产和免疫性不孕的细胞及其制备方法。所述靶向治疗习惯性流产和免疫性不孕的细胞的制备方法包括：无菌下抽取患者丈夫或血缘亲属或无关自愿健康个体静脉血约20ml左右，肝素抗凝；在无菌条件下，用等体积人淋巴细胞分离液加入离心管，再将抗凝的全血缓慢加入人淋巴细胞分离液上层，4℃、800～1200转/分钟缓升缓降离心；吸管吸取分离出的白膜层，生理盐水洗涤重悬后800～1200转/分钟离心，重复3次，去除残余的血清、红细胞等，将其制备成密度为(2～4)×10⁷/毫升的细胞混悬液，4℃暂存。本发明提高受孕率，减少流产率，使妊娠的成功率达到(70～80)％以上。 【EN】The invention belongs to the technical field of biological medicines, and discloses a cell for targeted treatment of habitual abortion and immune infertility and a preparation method thereof. The preparation method of the cell for targeted therapy of habitual abortion and immune infertility comprises the following steps: extracting venous blood of a husband or blood relatives or unrelated voluntary healthy individuals of the patient under aseptic condition by about 20ml, and anticoagulating heparin; under the aseptic condition, adding isovolumetric human lymphocyte separation fluid into a centrifuge tube, slowly adding anticoagulated whole blood into the upper layer of the human lymphocyte separation fluid, and slowly rising, slowly falling and centrifuging at 4 ℃ at 800-1200 r/min; sucking the separated white membrane layer by a suction pipe, washing with normal saline, resuspending, centrifuging at 800-1200 rpm, repeating for 3 times, and removing residuesThe serum and red blood cells of (2-4). times.10⁷Perml cell suspension, 4 ℃ temporary storage. The invention improves the conception rate, reduces the abortion rate and ensures that the success rate of pregnancy reaches more than (70-80)%.