摘要：【中文】本发明公开了一种检测镁离子的POSS‑大黄酸荧光探针及其制备方法，属于化学检测技术领域。本发明提供的POSS‑大黄酸荧光探针制备方法为：取POSS‑NH₃Cl粉末溶解在甲醇中，过阴离子树脂柱，得到POSS‑NH₂的甲醇溶液；再取大黄酸加入到POSS‑NH₂的甲醇溶液中，室温搅拌反应，反应溶液颜色由黄色变成橘色；用透析袋透析，结束后采用旋转蒸发仪将溶剂蒸发掉，得到紫色粉末，即为POSS‑大黄酸荧光探针。该荧光探针对镁离子有优异的选择性和灵敏性，环保无毒，具有广泛的应用前景。 【EN】The invention discloses a POSS-rhein fluorescent probe for detecting magnesium ions and a preparation method thereof, belonging to the technical field of chemical detection. The preparation method of the POSS-rhein fluorescent probe provided by the invention comprises the following steps: taking POSS-NH₃Dissolving Cl powder in methanol, and passing through an anion resin column to obtain POSS-NH₂The methanol solution of (4); adding rhein into POSS-NH₂Stirring the mixture at room temperature for reaction, and changing the color of the reaction solution from yellow to orange; dialyzing with a dialysis bag, and evaporating the solvent by using a rotary evaporator after the dialysis is finished to obtain purple powder, namely the POSS-rhein fluorescent probe. The fluorescent probe has excellent selectivity and sensitivity on magnesium ions, is environment-friendly and nontoxic, and has wide application prospect.

摘要：【中文】本发明公开了一种制备结构如I所示的雷美替胺中间体2‑(1,6,7,8‑四氢‑2H‑茚并[5,4‑b]呋喃‑8‑基)乙胺盐酸盐的方法，具体为：以2‑(1,2,6,7‑四氢‑8H‑茚并[5,4‑b]呋喃‑8‑亚基)乙腈(Ⅱ)为原料，在一定温度、催化剂以及溶剂条件下氢化一步法生成化含物I。本发明涉及的制备方法操作简便，避免了传统方法以下几个缺点：①反应条件苛刻；②反应时间过长，中间态Ⅲ转化不完全；③反应过程中产生大量聚合杂质A、B，反应转化率低。现有的合成路线和杂质A、B如下;；；。 【EN】The invention discloses a method for preparing ramelteon intermediate 2- (1,6,7, 8-tetrahydro-2H-indeno [5,4-b ] with a structure shown as I]The method for preparing furan-8-yl) ethylamine hydrochloride specifically comprises the following steps: with 2- (1,2,6, 7-tetrahydro-8H-indeno [5, 4-b)]The furan-8-ylidene) acetonitrile (II) is used as a raw material, and is hydrogenated to generate a compound I in a one-step method under the conditions of a certain temperature, a catalyst and a solvent, the preparation method related by the invention has simple and convenient operation, and avoids the defects of harsh ① reaction condition, overlong ② reaction time, incomplete conversion of intermediate state III, generation of a large amount of polymeric impurities A, B in the ③ reaction process, low reaction conversion rate, and the existing synthetic route and the impurities A, B are as follows;；；。

摘要：【中文】本发明涉及一种用于检测抗人体髓鞘碱性蛋白(MBP)抗体的试剂盒及其应用以及所述试剂盒用于抗人体髓鞘碱性蛋白抗体检测的方法，属于免疫检测领域。本发明提供了一种全新的患者血清或脑脊液中抗MBP自身抗体的检测方法，该方法通过MBP过表达载体构建、细胞转染、细胞免疫荧光检测等技术，进行抗体定性和滴度测定。本方法具有检测效率高，特异性强等特点。成功地解决了免疫印迹和酶联免疫吸附法中常见的检测结果假阳性或假阴性的问题，具有十分重要的临床应用价值。 【EN】The invention relates to a kit for detecting an anti-human body Myelin Basic Protein (MBP) antibody, application thereof and a method for detecting the anti-human body myelin basic protein antibody by using the kit, belonging to the field of immunoassay. The invention provides a brand-new method for detecting an anti-MBP autoantibody in serum or cerebrospinal fluid of a patient. The method has the characteristics of high detection efficiency, strong specificity and the like. Successfully solves the problem of false positive or false negative of the common detection result in the immunoblotting and enzyme-linked immunosorbent assay, and has very important clinical application value.

摘要：【中文】本发明公开了一种利用基质固相分散萃取固体或半固体食品中游离脂肪酸的方法，包含以下步骤，将氨基化分子筛、石英砂和食品研磨至混合均匀，得混合物；将氨基化分子筛作为固定相转移至固相萃取柱的空柱管中，再将所述混合柱转移至所述柱管中；使用淋洗液对所述固相萃取柱进行淋洗，再使用洗脱液对所述固相萃取柱进行洗脱，收集洗脱下的混合液，所述混合液中含有游离脂肪酸。使用气相色谱仪分析法(GC)对所得混合液中的游离脂肪酸进行分析。本发明实现了固体或半固体食品中游离脂肪酸的一步萃取；避免了对食品中的脂肪酸分析时，需要将食品提取成油脂后再进行游离脂肪酸的萃取，无需进行提取油脂的步骤，大幅缩短检测时间。 【EN】The invention discloses a method for extracting free fatty acid from solid or semisolid food by using matrix solid-phase dispersion, which comprises the following steps of grinding an aminated molecular sieve, quartz sand and the food until the mixture is uniformly mixed to obtain a mixture; transferring the aminated molecular sieve serving as a stationary phase into an empty column tube of a solid-phase extraction column, and transferring the mixed column into the column tube; and leaching the solid phase extraction column by using an eluent, eluting the solid phase extraction column by using an eluent, and collecting the eluted mixed solution, wherein the mixed solution contains free fatty acid. The resultant mixture was analyzed for free fatty acids using Gas Chromatography (GC). The invention realizes the one-step extraction of free fatty acid in solid or semisolid food; when fatty acid in food is analyzed, the food is required to be extracted into grease and then free fatty acid is extracted, the step of extracting the grease is not required, and the detection time is greatly shortened.

摘要：【中文】本发明公开了一种以甾醇、大豆卵磷脂、橄榄油为主要原料的甾醇油脂凝胶塑性脂肪的制备方法。本发明通过优化甾醇油脂凝胶塑性脂肪产品制备中甾醇与卵磷脂的最佳配比，得到的塑型脂肪既能在低温及常温下呈固态，在口腔温度下能具有良好的粘稠感。油脂凝胶是以液态植物油为连续相的有机凝胶，不需要经过氢化反应，不含有反式脂肪酸，能够替代传统由氢化方法制备得到塑性脂肪。本发明的油脂凝胶塑性脂肪的制备方法，能够为人造奶油等氢化油的生产开辟一条更为健康的新道路。 【EN】The invention discloses a preparation method of sterol oil gel plastic fat by taking sterol, soybean lecithin and olive oil as main raw materials. According to the invention, by optimizing the optimal ratio of sterol to lecithin in the preparation of the sterol oil gel plastic fat product, the obtained plastic fat can be in a solid state at low temperature and normal temperature, and has good viscous feeling at the oral temperature. The oil gel is an organogel taking liquid vegetable oil as a continuous phase, does not need hydrogenation reaction, does not contain trans-fatty acid, and can replace the traditional method for preparing plastic fat by a hydrogenation method. The preparation method of the oil gel plastic fat can open up a more healthy new way for the production of hydrogenated oil such as artificial butter.

摘要：【中文】本发明涉及医疗检测设备技术领域，尤其涉及一种实现信息交互的超声检测方法，具体包括：检测人员进行身份验证后启用掌上超声检测仪；检测人员采用掌上超声检测仪识别第二NFC标签以将第二关联信息传输至掌上超声检测仪内；掌上超声检测仪对多张超声图像以及第二关联信息中的患者的编号信息进行加密，并上传至一云服务器内；云服务器根据筛选出的超声图像和初步结果生成一初步处理报告后存储，同时云服务器根据初步处理报告更新编号信息，并将更新后的编号信息返回掌上超声检测仪内。有益效果：通过NFC功能实现自动录入患者信息，以节省检测人员录入数据的时间；同时，云服务器生成的初步处理报告并存储，以方便患者后续打印初步处理报告。 【EN】The invention relates to the technical field of medical detection equipment, in particular to an ultrasonic detection method for realizing information interaction, which specifically comprises the following steps: enabling the palm ultrasonic detector after the identity of the detection personnel is verified; the detection personnel adopts the handheld ultrasonic detector to identify the second NFC label so as to transmit the second associated information into the handheld ultrasonic detector; the handheld ultrasonic detector encrypts the serial number information of the patients in the multiple ultrasonic images and the second associated information and uploads the serial number information to a cloud server; and the cloud server generates and stores a primary processing report according to the screened ultrasonic images and the primary result, updates the number information according to the primary processing report, and returns the updated number information to the handheld ultrasonic detector. Has the advantages that: the patient information is automatically input through the NFC function, so that the time for the detection personnel to input data is saved; meanwhile, the primary processing report generated by the cloud server is stored, so that the primary processing report can be conveniently printed by the patient.

摘要：【中文】本发明提供一种植入式神经刺激设备，其中植入式神经刺激器包括：随机数生成器，用于依次产生随机数，相邻的所述随机数不相等，所述随机数用于指示脉冲的频率；脉冲输出电路，根据所述随机数依次输出随机频率的刺激脉冲。 【EN】The present invention provides an implantable neurostimulation device, wherein the implantable neurostimulator comprises: the random number generator is used for sequentially generating random numbers, the adjacent random numbers are not equal, and the random numbers are used for indicating the frequency of the pulse; and the pulse output circuit sequentially outputs stimulation pulses with random frequency according to the random number.

摘要：【中文】本发明涉及一种智慧管网巡检轨迹分布缩量存储查询方法，包括以下步骤：数据预定义处理，定义标准精度常量R、簇拥半径常量E，轨迹原始数据获取，按照p(lng,lat,w,r)格式标准封装；标准数据初步稀释，按照r数据值处理；簇拥抽稀，数据单元依据lng lat循环计算，按照值小于簇拥半径E簇拥分块，分块后计算每个簇拥块中心点及权重；数据转换，簇拥处理后数据转换为JSON数据，依据原始数据时间创建索引目录存储JSON数据并发布；数据查询，直接根据时间目录索引检索已发布JSON文件。本发明提供轨迹数据抽稀、存储及查询方法，解决了轨迹分析大数据量展现速度慢及渲染过载问题。 【EN】The invention relates to a storage and query method for distribution and shrinkage of routing inspection tracks of an intelligent pipe network, which comprises the following steps of: pre-defining data, defining a standard precision constant R and a cluster radius constant E, acquiring original track data, and packaging according to a p (lng, lat, w, R) format standard; preliminarily diluting the standard data, and processing according to the r data value; the cluster congestion is sparse, the data unit calculates circularly according to the lng lat, cluster blocks with the value smaller than the cluster radius E are divided, and the central point and the weight of each cluster block are calculated after the blocks are divided; data conversion, namely converting the data subjected to clustering processing into JSON data, creating an index directory according to the time of the original data to store the JSON data and releasing the JSON data; and (4) data query, namely retrieving the published JSON file directly according to the time directory index. The invention provides a track data thinning, storing and inquiring method, which solves the problems of slow display speed and rendering overload of track analysis large data volume.

摘要：【中文】本公开提供了一种科技资源协同评价耦合计算方法和设备，该方法包括：分别为待评价的科技资源设置若干个第一类指标值，并为每个设置的第一类指标值分配权重；计算出每个科技资源的综合归一化评价值；为每个科技资源设置若干个第二类指标值，为每个科技资源的第二类指标值和综合归一化评价值分配权重；计算出每个科技资源的二次综合归一化评价值；根据每个科技资源的二次综合归一化评价值计算每个科技资源之间的耦合协调度。以此方式，将空间因素加入到评价指标中，快速定量的给出不同科技资源之间的系统评价，提高了评价速度，避免了单个人为评价的主观不确定性，形成系统标准的评价体系。 【EN】The invention provides a scientific and technological resource collaborative evaluation coupling calculation method and device, wherein the method comprises the following steps: respectively setting a plurality of first-class index values for scientific and technological resources to be evaluated, and distributing weight for each set first-class index value; calculating a comprehensive normalized evaluation value of each scientific and technological resource; setting a plurality of second-class index values for each scientific and technological resource, and distributing weights for the second-class index values and the comprehensive normalized evaluation values of each scientific and technological resource; calculating a secondary comprehensive normalized evaluation value of each scientific and technical resource; and calculating the coupling co-scheduling between the scientific and technological resources according to the secondary comprehensive normalized evaluation value of each scientific and technological resource. In this way, the space factor is added into the evaluation index, the system evaluation among different scientific and technical resources is given out quickly and quantitatively, the evaluation speed is improved, the subjective uncertainty of single artificial evaluation is avoided, and an evaluation system of the system standard is formed.

摘要：【中文】本发明公开了一种用于检测AQP4抗体的细胞爬片及应用，所述细胞爬片是将HEK293T细胞贴壁生长在盖玻片上，再用含AQP4基因的pCMV‑HA‑AQP4表达质粒转染HEK293T细胞，获得表达AQP4蛋白的HEK293T细胞并固定；同时未转染AQP4蛋白HEK293T细胞固定于盖玻片上；然后将表达AQP4蛋白的HEK293T细胞盖玻片和未转染AQP4蛋白的HEK293T细胞盖玻片间隔固定在同一个载玻片的相同面上，分别作为反应区和对照区，获得所述细胞爬片。本方法可对细胞爬片成品细胞进行大量生产，且随取随用；检测人体内AQP4抗体的检验过程对设备的按要求相对较低，对技术要求低，时间花费少。 【EN】The invention discloses a cell slide for detecting AQP4 antibody and application, wherein the cell slide is prepared by adhering HEK293T cell on a cover glass, transfecting HEK293T cell with pCMV-HA-AQP4 expression plasmid containing AQP4 gene to obtain HEK293T cell expressing AQP4 protein and fixing; meanwhile, HEK293T cells which are not transfected with AQP4 protein are fixed on a cover glass; then HEK293T cell cover slip expressing AQP4 protein and HEK293T cell cover slip not transfected with AQP4 protein are fixed on the same surface of the same slide at intervals, and are respectively used as a reaction area and a control area to obtain the cell slide. The method can be used for mass production of finished cells of the cell climbing sheet, and can be taken at any time; the detection process for detecting the AQP4 antibody in the human body has relatively low requirements on equipment, low technical requirements and less time consumption.