摘要：【中文】本发明实施例提供一种弱网状态下机器人的应答方法、装置及机器人，该方法包括：当检测到第一询问请求时，上报第一询问请求；若在第一设定时长内未接收到第一询问请求对应的询问结果，则输出等待类语句；输出等待类语句后，若在第二设定时长内接收到询问结果，则根据询问结果，确定是否输出应答消息。本发明提供的弱网状态下机器人的应答方法、装置及机器人，以实现在弱网状态下，提高用户的体验。 【EN】The embodiment of the invention provides a response method and a response device of a robot in a weak network state and the robot, wherein the method comprises the following steps: when the first inquiry request is detected, reporting the first inquiry request; if the inquiry result corresponding to the first inquiry request is not received within the first set time length, outputting a waiting statement; and after the waiting statement is output, if the inquiry result is received within a second set time length, determining whether to output a response message according to the inquiry result. The robot response method and device in the weak network state and the robot provided by the invention can improve the user experience in the weak network state.

摘要：【中文】本发明公开了一种抗压型乳化炸药的制备方法，属于乳化炸药技术领域，该制备方法包括以下步骤：分别制备敏化剂和乳化基质，将敏化剂加入到乳化基质中混合均匀后获得乳化炸药；当装药温度为45‑60℃时，敏化剂包括亚硝酸钠、硝酸锌、硫氰酸钠和水；当装药温度为60‑90℃时，敏化剂包括亚硝酸钠、硝酸锌、硝酸钙和水。本发明提供的抗压型乳化炸药的制备方法，采用化学敏化方式进行乳化炸药的制备，提供的敏化剂可适应目前的中低温和高温两种敏化方式生产线，减少了不确定的安全因素，有效减少成本，有利于清洁生产，用该制备方法制备的抗压型乳化炸药具有爆速高、爆炸威力大、抗动压静压能力强等特点。 【EN】The invention discloses a preparation method of a pressure-resistant emulsion explosive, belonging to the technical field of emulsion explosives, and the preparation method comprises the following steps: respectively preparing a sensitizing agent and an emulsion matrix, adding the sensitizing agent into the emulsion matrix, and uniformly mixing to obtain an emulsion explosive; when the charging temperature is 45-60 ℃, the sensitizer comprises sodium nitrite, zinc nitrate, sodium thiocyanate and water; when the charging temperature is 60-90 ℃, the sensitizing agent comprises sodium nitrite, zinc nitrate, calcium nitrate and water. The preparation method of the pressure-resistant emulsion explosive provided by the invention adopts a chemical sensitization mode to prepare the emulsion explosive, the provided sensitizer can adapt to the current production lines of two sensitization modes of medium-low temperature and high temperature, uncertain safety factors are reduced, the cost is effectively reduced, the clean production is facilitated, and the pressure-resistant emulsion explosive prepared by the preparation method has the characteristics of high detonation velocity, large explosion power, strong dynamic pressure and static pressure resistance and the like.

摘要：【中文】本发明涉及制冷与空调产品试验装置技术领域，具体是涉及高精度全自动取样测量制冷剂含油率装置及测量方法。该装置包括取样容器、冷冻油收集装置、制冷剂收集装置、称重单元和数据处理模块，称重单元和数据处理模块电连接，取样容器连通被取样系统，用于盛放从被取样系统排放出来的由制冷剂和冷冻油组成的混合物，冷冻油收集装置和制冷剂收集装置均与取样容器连通。被取样系统的混合物流入到取样容器，该混合物由制冷剂和冷冻油组成，通过称重单元和数据处理模块获取混合物中冷冻油的重量，即制冷剂含油率。该制冷剂含油率能够对压缩机及系统的设计和生产的全过程进行一种客观的反应。 【EN】The invention relates to the technical field of refrigeration and air-conditioning product test devices, in particular to a high-precision full-automatic sampling device and a measuring method for measuring the oil content of a refrigerant. The device includes sample container, refrigeration oil collection device, refrigerant collection device, weighing unit and data processing module, and weighing unit and data processing module electricity are connected, and the sample container intercommunication is by sampling system for hold the mixture of being become by refrigerant and refrigeration oil that is discharged out by sampling system, refrigeration oil collection device and refrigerant collection device all communicate with sample container. The mixture of the sampled system flows into a sampling container, the mixture consists of refrigerant and refrigeration oil, and the weight of the refrigeration oil in the mixture, namely the oil content of the refrigerant, is obtained through a weighing unit and a data processing module. The oil content of the refrigerant can perform an objective reaction to the whole process of designing and producing the compressor and the system.

摘要：【中文】本发明公开了用于鉴别16种禽病病原冻干微芯片、试剂盒及方法，所述试剂盒包括一个冻干微芯片、一瓶矿物油、一管稀释液及一管无核酸酶水，所述冻干微芯片包被有引物、探针、Taq酶、反转录酶、海藻糖、Tris‑Cl、dNTP、Mg²⁺。冻干条件为：预冻阶段，隔板温度下降到‑55℃，预冻时保持时间为1h，然后设备进行抽真空，保持冷冻干燥1h；解析干燥阶段，再将隔板温度升至‑25℃保持1h，然后将隔板温度升至37℃并保持2h，最后将隔板降至25℃并保持1h，得到冻干微芯片，使用时加入稀释液和样本核酸即可。本发明同时检测16种禽病病原，所述检测试剂盒检测方法准确性、特异性和敏感性高，检测时间短。 【EN】The invention discloses a freeze-drying microchip, a kit and a method for identifying 16 avian disease pathogens, wherein the kit comprises the freeze-drying microchip, a bottle of mineral oil, a tube of diluent and a tube of nuclease-free water, and the freeze-drying microchip is coated with primers, probes, Taq enzyme, reverse transcriptase, trehalose, Tris-Cl, dNTP and Mg²⁺. The freeze-drying conditions are as follows: in the pre-freezing stage, the temperature of the partition plate is reduced to-55 ℃, the pre-freezing time is kept for 1h, then the equipment is vacuumized, and the freeze drying is kept for 1 h; analyzing and drying, heating the partition plate to-25 deg.C for 1 hr, and separatingThe temperature of the plate is increased to 37 ℃ and kept for 2h, and finally the temperature of the clapboard is reduced to 25 ℃ and kept for 1h to obtain the freeze-dried microchip, and the diluent and the sample nucleic acid are added when in use. The invention can detect 16 kinds of avian pathogens simultaneously, and the detection kit has high detection method accuracy, specificity and sensitivity and short detection time.

摘要：【中文】本发明“用于鉴别猪蓝耳病毒经典株与NADC30‑Like株的冻干微芯片、试剂盒及方法”，属于分子检测领域。所述冻干微芯片的特征是：用于鉴别猪蓝耳病毒经典株与NADC30‑Like株的荧光PCR反应体系通过冻干被固定在所述微芯片上；所述荧光PCR反应体系包括：下述引物和探针：猪蓝耳病毒经典株上游引物，猪蓝耳病毒经典株下游引物，猪蓝耳病毒经典株Taqman探针序列；NADC30‑Like株上游引物，NADC30‑Like株下游引物，NADC30‑Like株Taqman探针。本发明同时检测猪蓝耳经典株和NADC30‑Like株病毒，所述检测试剂盒检测方法准确性、特异性和敏感性高，检测时间短。 【EN】The invention discloses a freeze-drying microchip, a kit and a method for identifying classical strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and a strain NADC30-Like, belonging to the field of molecular detection. The freeze-drying microchip is characterized in that: a fluorescent PCR reaction system for identifying classical strains of porcine reproductive and respiratory syndrome virus and an NADC30-Like strain is fixed on the microchip by freeze-drying; the fluorescent PCR reaction system comprises: the following primers and probes: an upstream primer of a classical strain of the porcine reproductive and respiratory syndrome virus, a downstream primer of a classical strain of the porcine reproductive and respiratory syndrome virus, and a Taqman probe sequence of the classical strain of the porcine reproductive and respiratory syndrome virus; the kit comprises an NADC30-Like strain upstream primer, an NADC30-Like strain downstream primer and an NADC30-Like strain Taqman probe. The detection kit can be used for simultaneously detecting the porcine reproductive and respiratory syndrome classical strain and the NADC30-Like strain viruses, and the detection method of the detection kit has high accuracy, specificity and sensitivity and short detection time.

摘要：【中文】本发明“用于鉴别H5和H7亚型HA裂解位点插入变异型高致病性禽流感病毒的冻干微芯片、试剂盒及方法”，属于分子检测领域。所述冻干微芯片的特征是：荧光PCR反应体系通过冻干被固定在所述微芯片上；所述荧光PCR反应体系包括：H5亚型HA裂解位点插入变异型高致病性禽流感病毒上下游引物及Taqman探针；H7亚型HA裂解位点插入变异型高致病性禽流感病毒上下游引物及Taqman探针。本发明同时检测H5亚型HA裂解位点插入变异型高致病性禽流感病毒和H7亚型HA裂解位点插入变异型高致病性禽流感病毒，所述检测试剂盒检测方法准确性、特异性和敏感性高，检测时间短。 【EN】The invention discloses a freeze-drying microchip, a kit and a method for identifying insertion of H5 and H7 subtype HA cleavage sites into variant highly pathogenic avian influenza virus, and belongs to the field of molecular detection. The freeze-drying microchip is characterized in that: the fluorescent PCR reaction system is fixed on the microchip by freeze-drying; the fluorescent PCR reaction system comprises: the upstream and downstream primers and Taqman probes of the variant highly pathogenic avian influenza virus are inserted into the H5 subtype HA cleavage site; the upstream and downstream primers and Taqman probes of the variant highly pathogenic avian influenza virus are inserted into the H7 subtype HA cleavage site. The detection kit can be used for simultaneously detecting the insertion of the H5 subtype HA cleavage site into the variant highly pathogenic avian influenza virus and the insertion of the H7 subtype HA cleavage site into the variant highly pathogenic avian influenza virus, and HAs the advantages of high detection method accuracy, specificity and sensitivity and short detection time.

摘要：【中文】本发明“用于检测猪蓝耳病毒并鉴别从中猪蓝耳病毒高致病经典变异株的冻干微芯片、试剂盒及方法”，属于分子检测领域。用于检测猪蓝耳病毒并鉴别从中猪蓝耳病毒高致病经典变异株的荧光PCR反应体系通过冻干被固定在所述微芯片上；所述荧光PCR反应体系包括：猪蓝耳病毒株通用上游引物，猪蓝耳病毒株通用下游引物，猪蓝耳病毒株通用Taqman探针序列；高致病经典变异株上游引物；高致病经典变异株下游引物，高致病经典变异株Taqman探针序列。本发明可检测猪蓝耳病毒毒株和并可鉴别猪蓝耳病毒高致病经典变异株，所述检测试剂盒检测方法准确性、特异性和敏感性高，检测时间短。 【EN】The invention relates to a freeze-drying microchip, a kit and a method for detecting porcine reproductive and respiratory syndrome virus and identifying highly pathogenic classical variant strains of the porcine reproductive and respiratory syndrome virus, belonging to the field of molecular detection. A fluorescent PCR reaction system for detecting the porcine reproductive and respiratory syndrome virus and identifying a highly pathogenic classical variant strain of the porcine reproductive and respiratory syndrome virus is fixed on the microchip by freeze-drying; the fluorescent PCR reaction system comprises: universal upstream primers of the porcine reproductive and respiratory syndrome strains, universal downstream primers of the porcine reproductive and respiratory syndrome strains and universal Taqman probe sequences of the porcine reproductive and respiratory syndrome strains; the upstream primer of the highly pathogenic classical variant strain; downstream primers of highly pathogenic classical variant strains and Taqman probe sequences of highly pathogenic classical variant strains. The detection kit can detect the strain of the porcine reproductive and respiratory syndrome virus and can identify the highly pathogenic classical variant strain of the porcine reproductive and respiratory syndrome virus, and the detection method of the detection kit has high accuracy, specificity and sensitivity and short detection time.

摘要：【中文】本发明“用于鉴别H9和H6亚型低致病性禽流感病毒的冻干微芯片、试剂盒及方法”，属于分子检测领域。所述冻干微芯片的特征是：将用于鉴别H9和H6亚型低致病性禽流感病毒的荧光PCR反应体系通过冻干被固定在所述微芯片上；所述荧光PCR反应体系包括：下述引物和探针：禽流感H9亚型病毒上游引物，禽流感H9亚型病毒下游引物，禽流感H9亚型病毒Taqman探针；禽流感H6亚型病毒上游引物，禽流感H6亚型病毒下游引物，禽流感H6亚型病毒Taqman探针。本发明同时检测H9和H6亚型低致病性禽流感病毒，所述检测试剂盒检测方法准确性、特异性和敏感性高，检测时间短。 【EN】The invention discloses a freeze-drying microchip, a kit and a method for identifying H9 and H6 subtype low-pathogenicity avian influenza viruses, belonging to the field of molecular detection. The freeze-drying microchip is characterized in that: a fluorescent PCR reaction system for identifying the low-pathogenic avian influenza viruses of H9 and H6 subtypes is immobilized on the microchip by freeze-drying; the fluorescent PCR reaction system comprises: the following primers and probes: an avian influenza H9 subtype virus upstream primer, an avian influenza H9 subtype virus downstream primer and an avian influenza H9 subtype virus Taqman probe; avian influenza H6 subtype virus upstream primer, avian influenza H6 subtype virus downstream primer and avian influenza H6 subtype virus Taqman probe. The detection kit can be used for simultaneously detecting H9 and H6 subtype low-pathogenicity avian influenza viruses, and has the advantages of high accuracy, specificity and sensitivity and short detection time.

摘要：【中文】本发明公开了用于鉴别16种猪病病原冻干微芯片、试剂盒及方法，所述试剂盒包括一个冻干微芯片、一瓶矿物油、一管稀释液及一管无核酸酶水，所述冻干微芯片包被有引物、探针、Taq酶、反转录酶、海藻糖、Tris‑Cl、dNTP、Mg²⁺。冻干条件为：预冻阶段，隔板温度下降到‑55℃，预冻时保持时间为1h，然后设备进行抽真空，保持冷冻干燥1h；解析干燥阶段，再将隔板温度升至‑25℃保持1h，然后将隔板温度升至37℃并保持2h，最后将隔板降至25℃并保持1h，得到冻干微芯片，使用时加入稀释液和样本核酸即可。本发明同时检测16种猪病病原，所述检测试剂盒检测方法准确性、特异性和敏感性高，检测时间短。 【EN】The invention discloses a freeze-drying microchip, a kit and a method for identifying 16 swine disease pathogens, wherein the kit comprises the freeze-drying microchip, a bottle of mineral oil, a tube of diluent and a tube of nuclease-free water, and the freeze-drying microchip is coated with primers, probes, Taq enzyme, reverse transcriptase, trehalose, Tris-Cl, dNTP, Mg²⁺. The freeze-drying conditions are as follows: in the pre-freezing stage, the temperature of the partition plate is reduced to-55 ℃, the pre-freezing time is kept for 1h, then the equipment is vacuumized, and the freeze drying is kept for 1 h; analyzing and drying stage, increasing the temperature of the partition plate to-25 deg.C for 1 hr, then increasing the temperature of the partition plate to 37 deg.C for 2 hr, and finally decreasing the temperature of the partition plate to 25 deg.C for 1 hr to obtain lyophilized microchip, and usingThe diluent and the sample nucleic acid are added. The invention can detect 16 pig disease pathogens simultaneously, and the detection kit has high detection method accuracy, specificity and sensitivity and short detection time.