摘要：【中文】本发明涉及一种同时检测发酵液中L‑高丝氨酸和游离氨基酸的HPLC方法。所述方法是先对发酵液样品进行预处理，接着采用DEEMM衍生化试剂对发酵液样品进行衍生化处理之后，再通过高效液相色谱和通用型C18色谱柱对发酵液中的L‑高丝氨酸和其他游离氨基酸进行分离和测定。本发明的方法使用的配置是高效液相色谱系统的标准液相配置，除了适用于氨基酸分析之外，还可用于其他物质的分析工作，普适性更强，具有检测灵敏度高、样品处理简单、分析时间短、分析结果稳定、重复性好等优点，适用于常规实验室。 【EN】The invention relates to an HPLC method for simultaneously detecting L-homoserine and free amino acid in fermentation liquor. The method comprises the steps of pretreating a fermentation liquor sample, then adopting a DEEMM derivatization reagent to perform derivatization treatment on the fermentation liquor sample, and then separating and measuring L-homoserine and other free amino acids in the fermentation liquor by using high performance liquid chromatography and a universal C18 chromatographic column. The method provided by the invention adopts standard liquid phase configuration of a high performance liquid chromatography system, is suitable for amino acid analysis, can be used for analysis of other substances, has stronger universality, has the advantages of high detection sensitivity, simple sample treatment, short analysis time, stable analysis result, good repeatability and the like, and is suitable for conventional laboratories.

摘要：【中文】本发明涉及一种基于中国被毛孢代谢途径的培养方法。所述方法包括：将中国被毛孢菌种接种至发酵培养基，所述发酵培养基中添加关键差异代谢物质，在10～25℃、100～250rpm条件下培养培养216～240h，发酵结束后，发酵产物经过滤、烘干、研磨成粉后进行虫草酸、虫草素提取。本发明通过在中国被毛孢发酵培养前添加各种关键差异代谢物，可有效提高其主要活性化合物虫草酸与虫草素的含量。从本发明实施例中可以看出，在培养基中添加山梨糖醇可以使虫草酸的含量由59.28mg/g提高至149.38mg/g；在培养基中添加L‑脯氨酸或反式‑4‑羟基‑L‑脯氨酸可使虫草素含量分别由0.1103mg/g提高至0.1622mg/g或0.1687mg/g。 【EN】The invention relates to a culture method based on a hirsutella sinensis metabolic pathway. The method comprises the following steps: the method comprises the steps of inoculating hirsutella sinensis strains to a fermentation medium, adding key differential metabolites into the fermentation medium, culturing and culturing for 216-240 hours at 10-25 ℃ and 100-250 rpm, and after fermentation is finished, filtering, drying and grinding fermentation products into powder to extract cordycepic acid and cordycepin. According to the invention, various key differential metabolites are added before the fermentation culture of hirsutella sinensis, so that the contents of the main active compounds cordycepic acid and cordycepin can be effectively improved. As can be seen from the examples of the invention, the addition of sorbitol in the culture medium can increase the content of cordycepic acid from 59.28mg/g to 149.38 mg/g; the addition of L-proline or trans-4-hydroxy-L-proline in the culture medium can increase the cordycepin content from 0.1103mg/g to 0.1622mg/g or 0.1687mg/g, respectively.

摘要：【中文】本发明一种L‑半胱氨酸的基因工程菌及构建方法，以及该基因工程菌在微生物发酵制备L‑半胱氨酸中的应用。本发明通过(1)强化大肠杆菌L‑半胱氨酸合成途径的丝氨酸模块，增强大肠杆菌对丝氨酸的利用能力，(2)减弱丝氨酸的转运，减弱副产物对L‑半胱氨酸合成的影响，(3)增加硫模块合成基因的表达(4)质粒上异源表达Corynebacterium glutamicum的serC基因，得到L‑半胱氨酸高产的大肠杆菌基因工程菌株，L‑半胱氨酸产量从2.7g/L提高到3.83g/L。 【EN】The invention relates to a gene engineering bacterium of L-cysteine, a construction method and application of the gene engineering bacterium in preparing L-cysteine by microbial fermentation. According to the invention, the serine utilization capacity of escherichia coli to serine is enhanced by (1) strengthening a serine module of an escherichia coli L-cysteine synthesis way, (2) weakening the transfer of serine and weakening the influence of byproducts on L-cysteine synthesis, (3) increasing the expression of a sulfur module synthetic gene, (4) heterologously expressing the serC gene of Corynebacterium glutamicum on a plasmid, and obtaining the escherichia coli genetic engineering strain with high L-cysteine yield, wherein the L-cysteine yield is increased from 2.7g/L to 3.83 g/L.

摘要：【中文】本发明提供一种基于物联网的马铃薯无土栽培室和一种基于物联网的马铃薯无土栽培方法。一种基于物联网的马铃薯无土栽培室，包括栽培室主体、温度调节装置、物联网监控装置、栽培床、移动喷淋装置和遮光装置，温度调节装置和物联网监控装置配装在栽培室主体的外部，栽培室主体内部底板上固定若干栽培床，移动喷淋装置和遮光装置架设在栽培室主体内部，且遮光装置位于移动喷淋装置的上方，温度调节装置伸入栽培室主体内，温度调节装置、移动喷淋装置和遮光装置与物联网监控装置通信连接，物联网监控装置与监控终端通信连接，采用栽培室和该栽培方法能提高马铃薯的产量和质量。 【EN】The invention provides a potato soilless culture room based on the Internet of things and a potato soilless culture method based on the Internet of things. The utility model provides a potato soilless culture room based on thing networking, including the cultivation room main part, temperature regulation apparatus, thing networking monitoring device, the cultivation bed, remove spray set and shade, temperature regulation apparatus and thing networking monitoring device assemble in the outside of cultivation room main part, fixed a plurality of cultivation beds on the inside bottom plate of cultivation room main part, remove spray set and shade erects inside the cultivation room main part, and shade is located the top that removes spray set, temperature regulation apparatus stretches into in the cultivation room main part, temperature regulation apparatus, remove spray set and shade and thing networking monitoring device communication connection, thing networking monitoring device and monitor terminal communication connection, adopt cultivation room and this cultivation method can improve the output and the quality of potato.

摘要：【中文】本发明公开了一种酰胺酶突变体及其在催化合成2‑氯烟酸中的应用，属于酶工程技术领域。所述酰胺酶突变体由SEQ ID NO.1所示氨基酸序列的第378位、402位或403位进行单位点突变或多位点突变获得。本发明提供的酰胺酶突变体较亲本活力大幅提高，在使用该酶的粗提取物或工程菌全细胞进行催化时，反应酶活仍然保持较高状态。此外，本发明的酰胺酶突变体能够适应30～55℃的催化温度，为工业化酶法生产2‑氯烟酸奠定了基础。 【EN】The invention discloses an amidase mutant and application thereof in catalytic synthesis of 2-chloronicotinic acid, belonging to the technical field of enzyme engineering. The amidase mutant is obtained by carrying out single-site mutation or multi-site mutation on 378 th position, 402 th position or 403 th position of an amino acid sequence shown in SEQ ID NO. 1. Compared with parent strain, the amidase mutant provided by the invention has greatly improved activity, and the activity of the reaction enzyme is still kept in a higher state when a crude extract of the amidase or whole cells of engineering bacteria are used for catalysis. In addition, the amidase mutant provided by the invention can adapt to the catalytic temperature of 30-55 ℃, and lays a foundation for the industrial enzymatic production of 2-chloronicotinic acid.

摘要：【中文】本发明涉及一种提高基因工程菌泛酸产量的构建方法及菌株，以及该基因工程菌在微生物发酵制备D‑泛酸中的应用。本发明通过(1)加强lpd基因的表达(2)敲除glk、galP破坏葡萄糖非PTS转运系统，加强ptsG基因的表达而加强葡萄糖PTS转运系统(3)敲除yfbQ、ppsA基因(4)敲除poxB、pflB、ldhA基因(4)敲除ilvE基因(5)在质粒上引入异源的乙酰乳酸合酶基因alsS(6)在质粒上引入异源的泛酸转运体panT，最终得到最优的D‑泛酸高产大肠杆菌基因工程菌株。D‑泛酸效价从2.76g/L提高到6.33g/L。 【EN】The invention relates to a construction method and a strain for improving the yield of genetically engineered bacteria pantothenic acid, and application of the genetically engineered bacteria in preparation of D-pantothenic acid by microbial fermentation. The invention enhances the expression of lpd gene (1), knocks down glk and galP to destroy glucose non-PTS transport system, enhances the expression of ptsG gene to enhance the expression of glucose PTS transport system (3) knockdown yfbQ, ppsA gene (4) knockdown poxB, pflB and ldhA gene (4) knockdown ilvE gene (5), introduces heterologous acetolactate synthase gene alsS (6) on plasmid, introduces heterologous pantothenate transporter panT on plasmid, finally obtains the optimal D-pantothenate high-producing Escherichia coli genetic engineering strain. The D-pantothenic acid titer is increased from 2.76g/L to 6.33 g/L.

摘要：【中文】本发明公开了一种辣椒去柄机，可方便、连续去掉辣椒柄。包括机架，在机架上部水平方向设有传送带机构，在传送带机构上方设有与传送带机构相配的压紧带机构，在机架上位于传送带机构和压紧带机构的侧边设有皮带式夹柄机构；具有结构简单，可方便、连续去掉辣椒柄，减轻劳动强度，提高工作效率，省时省力的优点。 【EN】The invention discloses a hot pepper stem removing machine which can conveniently and continuously remove hot pepper stems. The automatic belt clamping device comprises a rack, wherein a conveying belt mechanism is arranged on the upper part of the rack in the horizontal direction, a belt pressing mechanism matched with the conveying belt mechanism is arranged above the conveying belt mechanism, and a belt type clamping handle mechanism is arranged on the rack and positioned on the side edges of the conveying belt mechanism and the belt pressing mechanism; has the advantages of simple structure, convenient and continuous removal of pepper stems, labor intensity reduction, work efficiency improvement, time saving and labor saving.

摘要：【中文】本发明公开了一种腈水解酶突变体及在普瑞巴林手性中间体合成中的应用，属于生物工程技术领域。本发明以芜菁/高山南芥腈水解酶嵌合体BaNIT的三突变体BaNITmut为亲本，运用定点突变对127位点和260位点进行改造，所获得的腈水解酶突变体氨基酸序列如SEQ ID NO.4或SEQ ID NO.6或SEQ ID NO.8。相比于亲本腈水解酶，本发明提供的腈水酶突变体催化外消旋异丁基丁二腈的活力及温度稳定性分别有所提高；同时副产物(S)‑3‑氰基‑5‑甲基己酰胺含量减少；操作易于控制，生产成本低，产品光学纯度高，在高效催化异丁基丁二腈合成(S)‑3‑氰基‑5‑甲基己酸中具有良好的应用前景。 【EN】The invention discloses a nitrilase mutant and application thereof in synthesis of pregabalin chiral intermediates, and belongs to the technical field of biological engineering. The invention takes three mutants of the turnip/southern mustard nitrilase chimera BanITmut as parents, and modifies 127 sites and 260 sites by site-directed mutagenesis, and the amino acid sequence of the obtained nitrilase mutant is shown as SEQ ID NO.4 or SEQ ID NO.6 or SEQ ID NO. 8. Compared with parent nitrilase, the nitrilase mutant provided by the invention has the advantages that the activity and the temperature stability of the catalysis of racemic isobutyl butanedinitrile are respectively improved; simultaneously, the content of the by-product (S) -3-cyano-5-methylhexanamide is reduced; the operation is easy to control, the production cost is low, the optical purity of the product is high, and the method has good application prospect in the synthesis of (S) -3-cyano-5-methylhexanoic acid by efficiently catalyzing isobutyl succinonitrile.

摘要：【中文】为解决现有方法制备得到的氟化镱纯度和金属离子元素含量无法达到高性能氟化物光学玻璃添加剂的要求的技术问题，本发明提供了一种高纯度光学玻璃添加剂氟化镱的制备方法，包括步骤：1、合成、纯化硝酸镱；2、浓缩，制备硝酸镱结晶；3、制备碳酸镱；4、合成氟化镱；5、赶净二氧化碳；6、焙烧氟化镱。本发明通过特殊的梯度温度对氟化镱滤饼进行焙烧，使氟化镱灼烧失重为0.07％‑0.2％，从而使制备得到的氟化镱纯度能达到99.90％‑99.95％。本发明通过对硝酸镱和碳酸钠进行纯化，使其含有的金属离子元素Fe、Cu、Ni、Cr、Co、Mn、Ti、V、Pb均小于0.3ppm，从而使最终制备得到的氟化镱中Fe、Cu、Ni、Cr、Co、Mn、Ti、V、Pb均小于1ppm。 【EN】In order to solve the technical problem that the purity and the content of metal ion elements of ytterbium fluoride prepared by the existing method can not meet the requirements of a high-performance fluoride optical glass additive, the invention provides a preparation method of ytterbium fluoride serving as a high-purity optical glass additive, which comprises the following steps: 1. synthesizing and purifying ytterbium nitrate; 2. concentrating to prepare ytterbium nitrate crystal; 3. preparing ytterbium carbonate; 4. synthesizing ytterbium fluoride; 5. removing carbon dioxide; 6. and (5) roasting ytterbium fluoride. The invention roasts the ytterbium fluoride filter cake through special gradient temperature, so that the ignition weight loss of the ytterbium fluoride is 0.07-0.2%, and the purity of the prepared ytterbium fluoride can reach 99.90-99.95%. According to the invention, ytterbium nitrate and sodium carbonate are purified, so that metal ion elements Fe, Cu, Ni, Cr, Co, Mn, Ti, V and Pb contained in ytterbium nitrate are all less than 0.3ppm, and Fe, Cu, Ni, Cr, Co, Mn, Ti, V and Pb in the finally prepared ytterbium fluoride are all less than 1 ppm.

摘要：【中文】本发明公开了一种负极片、其制备方法和锂离子电池，负极片包括负极集流体，负极集流体上涂布有负极浆料形成负极涂膜区，负极涂膜区的至少一侧边缘涂布有绝缘浆料形成绝缘涂膜区，绝缘涂膜区和负极涂膜区毗邻或部分互溶；绝缘涂膜区远离负极涂膜区的一侧边缘留有用于形成负极极耳的负极空白区，绝缘涂膜区位于负极极耳的根部。本发明在负极涂膜区的边缘增设绝缘涂膜区，可防止负极片边缘漏箔或厚边起鼓，并且可增强负极极耳根部的刚性轻度，防止负极极耳打皱和下塌，规避负极极耳翻折倒插至电芯内部覆盖负极材料导致的电池潜在析锂风险，并在极耳成型、卷绕或者叠片组装时提高工艺的操作性，降低不良，提高生产优率和生产效率。 【EN】The invention discloses a negative plate, a preparation method thereof and a lithium ion battery, wherein the negative plate comprises a negative current collector, negative slurry is coated on the negative current collector to form a negative film coating area, at least one side edge of the negative film coating area is coated with insulating slurry to form an insulating film coating area, and the insulating film coating area and the negative film coating area are adjacent or partially mutually soluble; and a negative pole blank area for forming a negative pole lug is reserved at the edge of one side of the insulating film coating area, which is far away from the negative pole film coating area, and the insulating film coating area is positioned at the root of the negative pole lug. The edge of the negative pole coating area is additionally provided with the insulating coating area, so that the edge of a negative pole piece can be prevented from leaking foil or bulging thick edges, the rigidity and the light degree of the root part of a negative pole lug can be enhanced, the negative pole lug is prevented from wrinkling and collapsing, the potential lithium precipitation risk of a battery caused by the fact that the negative pole lug is folded and inserted into a battery core to cover a negative pole material is avoided, the operability of the process is improved during the forming, winding or lamination assembly of the pole lug, the defects are reduced, and the production goodness and the production efficiency are improved.