摘要：【中文】本发明提供一种心肌细胞分离培养方法，包含以下步骤：1）取心脏组织，使用PBS液体清洗掉残留的血液；用眼科剪将心脏组织剪成0.1‑1mm³的组织块，加入0.5‑5mL的Ⅰ型胶原酶，36‑37.5℃条件下震荡消化0.5‑3小时；消化结束后加入2.5‑50mL PBS进行稀释中止，1200rpm/min离心5min，弃上清，获得细胞沉淀；2）将所述细胞沉淀用0.5‑5mL胰酶进行重悬，弯头吸管吹打10‑300次，消化液变浑浊后加入培养液终止胰酶消化，1200rpm/min离心5min，弃上清，获得原代心肌细胞。3）将所述原代心肌细胞用培养液进行重悬后，按照0.1‑10×10⁵个细胞/ cm²接种于培养液中，置于37℃、5%CO₂的培养箱中进行培养，获得所述心肌细胞。 【EN】The invention provides a myocardial cell separation culture method, which comprises the following steps: 1) taking heart tissue, and washing residual blood by using PBS liquid; cutting cardiac tissue into 0.1-1mm with ophthalmic scissors³Adding 0.5-5mL of collagenase type I into the tissue mass, and digesting the tissue mass for 0.5-3 hours at 36-37.5 ℃ by shaking; after digestion, adding 2.5-50mL of PBS for dilution stopping, centrifuging at 1200rpm/min for 5min, and removing supernatant to obtain cell sediment; 2) and (3) resuspending the cell sediment by using 0.5-5mL of pancreatin, blowing the cell sediment by using an elbow suction tube for 10-300 times, adding a culture solution after the digestive juice becomes turbid to terminate the pancreatin digestion, centrifuging the cell sediment for 5min at 1200rpm/min, and removing the supernatant to obtain the primary cardiomyocytes. 3) Resuspending the primary cardiomyocytes in a culture medium at a ratio of 0.1-10X 10⁵Individual cell/cm²Inoculating to culture medium, and standing at 37 deg.C and 5% CO₂Culturing in the incubator to obtain the cardiomyocytes.

摘要：【中文】本发明公开了一种基于辅助支撑机械臂实现的椰子采摘方法，辅助支撑机械包括车架、车架上端所设的固定支撑座、固定支撑座上端所设的液压缸I、液压缸I上端所设的固定台、固定台上端所设的环形转台与环形转台侧面所匹配安装的移动架，所述车架的左右两端对称设有四个车轮，所述固定支撑座的上端面上设有方形结构的液压缸I；移动架匹配安装在环形转台上并且与环形转台一同在液压缸I的驱动下升降移动，这种结构使得采摘工人可以轻松的到达所需采摘的任意高度位置，避免了攀爬的难度与高空攀爬的危险性；工人在移动架内面对椰子进行采摘，避免了高空采摘的危险性，并且可以全身心的专注与采摘工作，提高了采摘的效率。 【EN】The invention discloses a coconut picking method based on an auxiliary supporting mechanical arm, wherein the auxiliary supporting mechanical arm comprises a frame, a fixed supporting seat arranged at the upper end of the frame, a hydraulic cylinder I arranged at the upper end of the fixed supporting seat, a fixed table arranged at the upper end of the hydraulic cylinder I, an annular turntable arranged at the upper end of the fixed table and a movable frame arranged on the side surface of the annular turntable in a matched manner, four wheels are symmetrically arranged at the left end and the right end of the frame, and the hydraulic cylinder I with a square structure is arranged on the upper end surface of the fixed supporting seat; the movable frame is arranged on the annular rotary table in a matched mode and is driven by the hydraulic cylinder I to lift and move together with the annular rotary table, so that picking workers can easily reach any height position needing picking through the structure, and the climbing difficulty and the high-altitude climbing danger are avoided; the workman picks to the coconut in moving the frame, has avoided the danger of high altitude picking to can be whole body and mind be absorbed in and pick work, improved the efficiency of picking.

摘要：【中文】本发明公开了一种污泥高效资源化利用系统及其处理方法，该系统依次由破壁热水解装置、厌氧预消化装置、机械深度脱水装置、污泥破碎装置、污泥余热干化单元和污泥焚烧单元组成；其处理方法主要为破壁反应、厌氧预消化反应、深度脱水处理、干化处理、焚烧处理处置；资源化利用包括有机质资源化利用和无机质资源化利用。本发明对系统控制要求简单、易操作，不投加调理剂、不增加污泥量、运行费用低，利用系统产生的热能、电力来降低运行能耗，污泥最终资源化利用处置，无二次污染、不占用土地、使用不受限制，全面实现污泥″减量化、无害化、资源化″。 【EN】The invention discloses a high-efficiency resource utilization system of sludge and a treatment method thereof, wherein the system sequentially comprises a wall-breaking thermal hydrolysis device, an anaerobic pre-digestion device, a mechanical deep dehydration device, a sludge crushing device, a sludge waste heat drying unit and a sludge incineration unit; the treatment method mainly comprises the steps of wall breaking reaction, anaerobic predigestion reaction, deep dehydration treatment, drying treatment and incineration treatment; the resource utilization comprises organic matter resource utilization and inorganic matter resource utilization. The invention has simple control requirement on the system, easy operation, no addition of conditioner, no increase of sludge amount and low operation cost, reduces operation energy consumption by utilizing heat energy and electric power generated by the system, finally realizes resource utilization and disposal of sludge, has no secondary pollution, occupies no land, is not limited in use, and comprehensively realizes reduction, harmlessness and resource utilization of the sludge.

摘要：【中文】本发明涉及一种锂电池高效散热装置，包括：电池机箱，电池BMS，还包括：复合材料装置，金属材料装置；所述电池BMS在电池机箱上方，所述复合材料装置在金属材料装置上方。与现有技术相比，保证了电池内部锂电芯、BMS元器件较低的工作温度，降低析锂风险，改善电池性能一致性、循环寿命和安全性能。 【EN】The invention relates to a high-efficiency heat dissipation device of a lithium battery, which comprises: battery machine case, battery BMS still includes: composite material devices, metallic material devices; the battery BMS is above the battery case, and the composite material device is above the metal material device. Compared with the prior art, the lithium battery has the advantages that the lower working temperature of the lithium battery core and the BMS component inside the battery is guaranteed, the risk of lithium precipitation is reduced, and the consistency of the battery performance, the cycle life and the safety performance are improved.

摘要：【中文】本发明公开了椰油酰胺丙基PG‑二甲基氯化铵作为抑菌剂的应用及其作为抑菌剂在日化用品中的应用。与现有技术相比，本发明提供了椰油酰胺丙基PG‑二甲基氯化铵作为抑菌剂的新用途，且椰油酰胺丙基PG‑二甲基氯化铵作为抑菌剂可以应用在日化用品领域，起到良好的抑菌效果。 【EN】The invention discloses application of cocamidopropyl PG-dimethyl ammonium chloride as a bacteriostatic agent and application of the cocamidopropyl PG-dimethyl ammonium chloride as the bacteriostatic agent in daily chemical products. Compared with the prior art, the invention provides the new application of the cocamidopropyl PG-dimethyl ammonium chloride as the bacteriostatic agent, and the cocamidopropyl PG-dimethyl ammonium chloride as the bacteriostatic agent can be applied to the field of daily chemical products and has good bacteriostatic effect.

摘要：【中文】本申请提供了一种信息推送方法，其中，该方法包括：获取第一区域中未接单的目标司机；判断所述目标司机是否满足预设推送条件；若满足所述预设推送条件，向所述目标司机发送第一推送消息，所述第一推送消息包括第二区域的第二区域信息，所述第二区域为，其中，所述第一区域的第一区域热度低于所述第二区域的第二区域热度。 【EN】The application provides an information pushing method, wherein the method comprises the following steps: acquiring a target driver who does not receive a bill in a first area; judging whether the target driver meets a preset pushing condition or not; and if the preset pushing condition is met, sending a first pushing message to the target driver, wherein the first pushing message comprises second area information of a second area, and the second area is the second area, wherein the first area heat of the first area is lower than the second area heat of the second area.

摘要：【中文】本发明公开了一种椰油酰胺丙基PG‑二甲基氯化铵与多元醇的复配物，其特征在于：包括有椰油酰胺丙基PG‑二甲基氯化铵水溶液和多元醇，且所述椰油酰胺丙基PG‑二甲基氯化铵水溶液与所述多元醇的质量比为1:10～10:1。本发明还公开了椰油酰胺丙基PG‑二甲基氯化铵与多元醇的复配物作为抑菌剂的应用以及作为抑菌剂在日化用品中的应用。与现有技术相比，本发明的优点在于：本发明提供了一种椰油酰胺丙基PG‑二甲基氯化铵与多元醇的复配物，椰油酰胺丙基PG‑二甲基氯化铵与多元醇可以起到协同增效的作用，提高抑菌效果。 【EN】The invention discloses a compound of cocamidopropyl PG-dimethyl ammonium chloride and polyalcohol, which is characterized in that: the composite material comprises a cocamidopropyl PG-dimethyl ammonium chloride aqueous solution and polyhydric alcohol, wherein the mass ratio of the cocamidopropyl PG-dimethyl ammonium chloride aqueous solution to the polyhydric alcohol is 1: 10-10: 1. The invention also discloses application of the complex of the cocamidopropyl PG-dimethylammonium chloride and the polyalcohol as a bacteriostatic agent and application of the complex as the bacteriostatic agent in daily chemical products. Compared with the prior art, the invention has the advantages that: the invention provides a compound of cocamidopropyl PG-dimethylammonium chloride and polyalcohol, wherein the cocamidopropyl PG-dimethylammonium chloride and the polyalcohol can play a role in synergy and improve the antibacterial effect.

摘要：【中文】本发明公开了一种基于苯并噻唑联杂环的半花菁类化合物及其制备方法与应用。该类化合物的结构式式如式I所示：该化合物的制备方法，包括如下步骤：以式Ⅱ所示的苯并噻唑类化合物与卤代烷R^bX进行烷基化反应，得到取代的苯并噻唑类化合物，然后与N‑乙基咔唑‑3‑甲醛进行Knoevenagel缩合反应，即得。本发明合成制备步骤简单易行、原料廉价易得，作为探针结构稳定、具有高特异性，可用于特异性检测核酸的G‑四链体和双螺旋的二级结构。所述探针对核酸二级结构的检测方法具有价格低廉、操作简便，选择性好，快捷的优点，克服了其他检测方法价格昂贵、设备要求高及技术操作相对复杂的缺点。 【EN】The invention discloses a hemicyanine compound based on benzothiazole linked heterocycle and a preparation method and application thereof. The structural formula of the compound is shown as formula I:the preparation method of the compound comprises the following steps: benzothiazole compound shown as formula II and alkyl halide R^bPerforming alkylation reaction on the X to obtain a substituted benzothiazole compound, and then performing Kno reaction on the substituted benzothiazole compound and N-ethylcarbazole-3-formaldehydeAnd performing evenagel condensation reaction to obtain the compound. The invention has simple and easy synthetic preparation steps, cheap and easily obtained raw materials, stable structure as a probe, high specificity and capability of being used for specifically detecting the secondary structure of G-quadruplex and double helix of nucleic acid. The method for detecting the secondary structure of the nucleic acid by the probe has the advantages of low price, simple and convenient operation, good selectivity and rapidness, and overcomes the defects of high price, high equipment requirement and relatively complex technical operation of other detection methods.

摘要：【中文】本发明公开了一种滤珠水质澄清过滤装置及其使用方法，包括搅拌装置、防短流出水渠、滤板、滤头、滤珠、配水装置；配水装置在污泥区上方，且与进水管相连；排泥装置在污泥区中，且与排泥管相连；缓冲区在污泥区上方；滤珠的过滤层位于缓冲区上方；滤珠的过滤层上方为清水区，且中间有滤板隔断，滤板开孔处有滤头，污水通过滤头到达清水区；清水区上方为防短流出水渠，且与出水管相连。澄清时，通过进水管向设备内进水，后通过配水装置均匀布水，滤珠可以物理澄清水中的杂质，澄清后的水通过滤头通往清水区，最终从出水管排出；反洗时，首先关闭进水，然后打开排泥管阀门，且同步打开搅拌装置，所有的污水经过排泥装置，最终从排泥管排出。 【EN】The invention discloses a filter bead water quality clarification and filtration device and a use method thereof, wherein the device comprises a stirring device, a short-flow-preventing water outlet channel, a filter plate, a filter head, filter beads and a water distribution device; the water distribution device is arranged above the sludge area and is connected with the water inlet pipe; the sludge discharge device is arranged in the sludge area and is connected with the sludge discharge pipe; the buffer zone is arranged above the sludge zone; the filter layer of the filter beads is positioned above the buffer zone; a clear water area is arranged above a filter layer of the filter beads, a filter plate partition is arranged in the middle of the filter layer, a filter head is arranged at an opening of the filter plate, and sewage reaches the clear water area through the filter head; the upper part of the clear water area is provided with a short-flow-preventing water outlet channel which is connected with a water outlet pipe. During clarification, water is fed into the equipment through the water inlet pipe, then water is uniformly distributed through the water distribution device, the filter beads can physically clarify impurities in the water, and the clarified water is led to a clear water area through the filter head and finally discharged from the water outlet pipe; during backwashing, the water inlet is closed firstly, then the valve of the sludge discharge pipe is opened, the stirring device is synchronously opened, and all sewage passes through the sludge discharge device and is finally discharged from the sludge discharge pipe.

摘要：【中文】本发明涉及酸化罐技术领域，且公开了一种基于陶瓷膜工艺的循环式分离系统，包括酸化罐以及陶瓷膜，所述酸化罐的输出口通过管道连通陶瓷膜，陶瓷膜的输出口通过管道连通酸化罐，陶瓷膜分离后含有菌渣的浓浆，再次进入酸化罐，再次通过循环进入陶瓷膜，反复循环，当电机开设工作的时候的，电机带动连接柱旋转，连接柱带动活动套旋转，活动套旋转带动搅拌叶搅拌，然后对罐体内部的原液进行搅拌，在搅拌的过程中，连杆会顺着三角块的斜面逐渐向上移动，当经过三角块的时候，连杆会向下移动，连杆的上下移动，会带动活动套的上下移动，活动套的上下移动可以带动搅拌叶进行上下震动搅拌，以此可以对原液进行充分搅拌。 【EN】The invention relates to the technical field of acidification tanks, and discloses a circulating separation system based on a ceramic membrane process, which comprises an acidification tank and a ceramic membrane, wherein an output port of the acidification tank is communicated with the ceramic membrane through a pipeline, an output port of the ceramic membrane is communicated with the acidification tank through a pipeline, thick slurry containing bacterial residues after the ceramic membrane is separated enters the acidification tank again, enters the ceramic membrane again through circulation, and circulates repeatedly, when a motor is started to work, the motor drives a connecting column to rotate, the connecting column drives a movable sleeve to rotate, the movable sleeve rotates to drive stirring blades to stir, then stock solution in a tank body is stirred, in the stirring process, a connecting rod can gradually move upwards along the inclined plane of a triangular block, when the connecting rod passes through the triangular block, the connecting rod can move downwards, the connecting rod can move upwards and downwards to drive the movable sleeve to move upwards and downwards, the movable sleeve can move upwards and downwards to drive, thereby, the stock solution can be sufficiently stirred.