摘要：【中文】本发明涉及医疗器械体外诊断试剂领域，特别涉及一种全自动化学发光测定仪专用的清洗液或其稀释液及其制备方法。该清洗液包含无机盐、磷酸盐缓冲体系、表面活性剂1、表面活性剂2、防腐剂和水；无机盐由氯化钠和氯化钾组成；磷酸盐缓冲体系为磷酸氢二钠‑磷酸二氢钾体系、磷酸二氢钠‑磷酸氢二钾中的一种；表面活性剂1为吐温‑20、吐温‑80中的一种；表面活性剂2为十二烷基硫酸钠、月桂酸钠中的一种；防腐剂由Proclin300和Bronidox组成。本发明提供的清洗液，配制工艺简单，成本低，适用于不同厂家的全自动化学发光测定仪，同时适用于不同检测原理项目的检测，并且准确度好、精密性高、防腐性能好。 【EN】The invention relates to the field of in-vitro diagnostic reagents for medical instruments, in particular to a special cleaning solution or diluent thereof for a full-automatic chemiluminescence determinator and a preparation method thereof. The cleaning solution comprises inorganic salt, a phosphate buffer system, a surfactant 1, a surfactant 2, a preservative and water; the inorganic salt consists of sodium chloride and potassium chloride; the phosphate buffer system is one of a disodium hydrogen phosphate-potassium dihydrogen phosphate system and sodium dihydrogen phosphate-dipotassium hydrogen phosphate; the surfactant 1 is one of tween-20 and tween-80; the surfactant 2 is one of sodium dodecyl sulfate and sodium laurate; the preservative consisted of Proclin300 and Bronidox. The cleaning solution provided by the invention is simple in preparation process, low in cost, suitable for full-automatic chemiluminescence testers of different manufacturers, suitable for detection of different detection principle items, good in accuracy, high in precision and good in corrosion resistance.

摘要：【中文】本发明属于生物技术领域，具体涉及一种热稳定性提高的胺脱氢酶突变体及其基因工程菌的构建和应用。本发明提供的胺脱氢酶突变体包括4种单点突变体和11种组合突变体，与野生型胺脱氢酶相比，其单点突变体和组合突变体在42℃下半衰期均更长；尤其是组合突变体，表现出单点突变体热稳定性的叠加效果，其半衰期大约是野生型脱氨氢酶的5倍。通过本发明所提供的构建方法得到的胺脱氢酶突变体的热稳定性更好，在较高的温度下催化合成手性胺时，表现出优良的立体选择性、区域选择性和催化活性，具有较好的应用前景。 【EN】The invention belongs to the technical field of biology, and particularly relates to an amine dehydrogenase mutant with improved thermal stability and construction and application of a genetic engineering bacterium thereof. The amine dehydrogenase mutant provided by the invention comprises 4 single-point mutants and 11 combined mutants, and compared with wild amine dehydrogenase, the single-point mutants and the combined mutants have longer half lives at 42 ℃; in particular, the combination mutant showed a superimposed effect of thermal stability of the single-point mutant, with a half-life of approximately 5 times that of the wild-type deaminase. The amine dehydrogenase mutant obtained by the construction method provided by the invention has better thermal stability, shows excellent stereoselectivity, regioselectivity and catalytic activity when catalyzing and synthesizing chiral amine at higher temperature, and has better application prospect.

摘要：【中文】本发明涉及一种双偏心齿轮偏心面的同步检测方法，包括如下步骤：步骤一、测量第一偏心体弧面与齿轮内圆的最小距离；步骤二、找到齿轮上距第一偏心体最小距离的齿槽或齿顶A；以A为定点，分别沿顺时针和逆时针数相同数量的轮齿而找到位置B和位置E，AB弧和AE弧所对应的圆心角最接近90°或等于90°，测量B和C是否对称；步骤三、测量第二偏心体弧面与齿轮内圆的最小距离；步骤四、找到齿轮上距第二偏心体最小距离的齿槽a；则以a为定点，分别沿顺时针和逆时针数相同数量的轮齿而找到位置b和位置e，ab弧和ae弧所对应的圆心角最接近90°或等于90°；步骤五、使用百分表测量装置测量第一偏心体和第二偏心体距A的最小距离是否相等。 【EN】The invention relates to a synchronous detection method for eccentric surfaces of double eccentric gears, which comprises the following steps: measuring the minimum distance between the cambered surface of the first eccentric body and the inner circle of the gear; step two, finding a tooth socket or tooth top A which is the minimum distance away from the first eccentric body on the gear; taking A as a fixed point, respectively finding a position B and a position E along the gear teeth with the same number of clockwise and counterclockwise needles, wherein central angles corresponding to AB arcs and AE arcs are closest to 90 degrees or equal to 90 degrees, and measuring whether B and C are symmetrical or not; measuring the minimum distance between the cambered surface of the second eccentric body and the inner circle of the gear; step four, finding the tooth socket a which is on the gear and has the minimum distance from the second eccentric body; using a as a fixed point, respectively finding a position b and a position e along the gear teeth with the same number of clockwise and counterclockwise needles, wherein the central angles corresponding to the ab arc and the ae arc are closest to 90 degrees or equal to 90 degrees; and step five, measuring whether the minimum distances from the first eccentric body to the A are equal to the minimum distances from the second eccentric body to the A by using a dial indicator measuring device.

摘要：【中文】本发明涉及一种胺脱氢酶突变体、酶制剂、重组载体、重组细胞及其制备方法和应用。该胺脱氢酶突变体，由野生型胺脱氢酶的第104位的谷氨酸突变为丙氨酸后得到；或者，由野生型胺脱氢酶的第137位的天冬氨酸突变为脯氨酸后得到；或者，由野生型胺脱氢酶的第174位的缬氨酸突变为半胱氨酸后得到；或者，由野生型胺脱氢酶的第197位的缬氨酸突变为天冬氨酸后得到。上述胺脱氢酶的催化效率较高。 【EN】The invention relates to an amine dehydrogenase mutant, an enzyme preparation, a recombinant vector, a recombinant cell, a preparation method and an application thereof. The amine dehydrogenase mutant is obtained by mutating 104 th glutamic acid of wild amine dehydrogenase into alanine; or obtained by mutating aspartic acid at 137 th site of wild amine dehydrogenase into proline; or the valine at the 174 th position of the wild amine dehydrogenase is mutated into the cysteine to obtain the amino acid; or mutation from valine at position 197 of wild-type amine dehydrogenase to aspartic acid. The catalytic efficiency of the amine dehydrogenase is high.

摘要：【中文】本发明属于生物技术领域，具体涉及一种转氨酶突变体及其构建方法和应用。本发明提供的转氨酶突变体包括在野生型转氨酶(WP_0532423951.1)氨基酸序列的第259位、第431位、第264位、第269位及第381位进行单点突变和组合突变的多种突变体，与野生型转氨酶(WP_0532423951.1)相比，该突变体在55℃下半衰期更长，最优组合突变体的半衰期大约是野生型转氨酶(WP_0532423951.1)的5倍，热稳定性更好，适于在较高的温度下催化合成手性胺。通过本发明所提供的构建方法得到的转氨酶突变体在较高的温度下催化合成手性胺时表现出优良的立体选择性和催化活性，具有较好的应用前景。 【EN】The invention belongs to the technical field of biology, and particularly relates to a transaminase mutant and a construction method and application thereof. The transaminase mutant provided by the invention comprises a plurality of mutants which are subjected to single-point mutation and combined mutation at 259, 431, 264, 269 and 381 of the amino acid sequence of wild-type transaminase (WP _0532423951.1), and compared with the wild-type transaminase (WP _0532423951.1), the mutant has longer half-life at 55 ℃, the half-life of the optimal combined mutant is about 5 times that of the wild-type transaminase (WP _0532423951.1), the heat stability is better, and the mutant is suitable for catalytically synthesizing chiral amine at higher temperature. The transaminase mutant obtained by the construction method provided by the invention has excellent stereoselectivity and catalytic activity when catalyzing and synthesizing chiral amine at higher temperature, and has better application prospect.

摘要：【中文】本发明提供了一种垃圾中转站渗滤液处理装置和工艺，属于污水处理技术领域，包括依次连接并形成循环回路的加药混凝池和气浮机，所述加药混凝池还分别与调节池以及铁碳微电解塔相连，所述气浮机一侧设置有空压机、溶气罐和溶气泵，所述空压机、溶气罐以及气浮机之间顺序连接，所述溶气罐、溶气泵以及气浮机之间顺序连接，所述铁碳微电解塔一侧设置有生化处理单元。本发明实施例解决了小水量无合适处理设备的问题，装置实现小型化和大水量，实现与大型设备的拼接，可适用于任何规模的垃圾中转站渗滤液处理，同时可采用配套全自动控制系统和相应的感应器，全程自动运行，具有投资和运行成本低、占地较小、处理效果稳定可靠的优点。 【EN】The invention provides a garbage transfer station leachate treatment device and a process, which belong to the technical field of sewage treatment, and comprise a dosing coagulation tank and an air floatation machine which are sequentially connected and form a circulation loop, wherein the dosing coagulation tank is also respectively connected with an adjusting tank and an iron-carbon micro-electrolysis tower, one side of the air floatation machine is provided with an air compressor, a dissolved air tank and a dissolved air pump, the air compressor, the dissolved air tank and the air floatation machine are sequentially connected, the dissolved air tank, the dissolved air pump and the air floatation machine are sequentially connected, and one side of the iron-carbon micro-electrolysis tower is provided with a biochemical treatment unit. The embodiment of the invention solves the problem that small water volume does not have proper treatment equipment, realizes miniaturization and large water volume, realizes splicing with large-scale equipment, is suitable for treating percolate of a garbage transfer station with any scale, can adopt a matched full-automatic control system and a corresponding sensor, automatically operates in the whole process, and has the advantages of low investment and operation cost, small occupied area and stable and reliable treatment effect.

摘要：【中文】本发明涉及一种转氨酶突变体、酶制剂、重组载体、重组细胞及其制备方法和应用。该转氨酶突变体的氨基酸序列由野生型转氨酶的氨基酸序列发生突变得到，野生型转氨酶的氨基酸序列如SEQ ID No.1所示，发生突变的氨基酸位点包括第226位氨基酸位点、第281位氨基酸位点、第312位氨基酸位点及第406位氨基酸位点中的至少一个。上述转氨酶突变体的酶活较高。 【EN】The invention relates to a transaminase mutant, an enzyme preparation, a recombinant vector, a recombinant cell, a preparation method and application thereof. The amino acid sequence of the transaminase mutant is obtained by mutating the amino acid sequence of wild-type transaminase, the amino acid sequence of the wild-type transaminase is shown as SEQ ID No.1, and the mutated amino acid position comprises at least one of 226 th amino acid position, 281 th amino acid position, 312 th amino acid position and 406 th amino acid position. The transaminase mutant has high enzyme activity.

摘要：【中文】本发明提供了一种小型微滤装置，属于污水处理技术领域，包括沉淀水箱，所述沉淀水箱侧壁上方设置有进水管，沉淀水箱内部设置有过滤组件，所述过滤组件包括滤布框架以及用于带动所述滤布框架转动的驱动组件，所述滤布框架外部设置有滤布，所述滤布框架底部通过旋转接头连接有出水管，所述出水管远离所述旋转接头的一端延伸至所述沉淀水箱外部，所述滤布框架外侧还设置有吸泥组件。本发明实施例实现了农村污水生化后污水的高效悬浮物去除的深度处理，并且可在原有清水箱进行改造，增加模块即可达到深度处理，同时很好的利用了重力作用减轻能耗；相较于传统MBR的过滤工艺，具有悬浮物去除效果好、效率高以及无需维护等优点。 【EN】The invention provides a small-sized microfiltration device, which belongs to the technical field of sewage treatment and comprises a precipitation water tank, wherein a water inlet pipe is arranged above the side wall of the precipitation water tank, a filtering assembly is arranged in the precipitation water tank, the filtering assembly comprises a filter cloth frame and a driving assembly for driving the filter cloth frame to rotate, filter cloth is arranged outside the filter cloth frame, the bottom of the filter cloth frame is connected with a water outlet pipe through a rotary joint, one end of the water outlet pipe, far away from the rotary joint, extends to the outside of the precipitation water tank, and a sludge suction assembly is further arranged outside the filter cloth frame. The embodiment of the invention realizes the advanced treatment of the sewage after the biochemical treatment of the rural sewage by efficiently removing suspended matters, can be reformed in the original clean water tank, can achieve the advanced treatment by adding modules, and simultaneously well utilizes the gravity action to reduce the energy consumption; compared with the traditional MBR filtering process, the method has the advantages of good suspended matter removing effect, high efficiency, no need of maintenance and the like.

摘要：【中文】本发明公开了一种电子设备信号的监测系统、方法以及装置。所述电子设备信号的监测系统包括：电子设备，安装在电子设备机内的监测设备，与监测设备连接的监测服务器；该电子设备机内设有测试点；该监测设备设有采集通道，该采集通道用于连接电子设备机内的测试点。所述电子设备信号的监测方法包括：通过采集通道对电子设备机内的各测试点进行信号采集，获得对应的监测信号；解析监测信号得到对应的测量数据，并将测量数据发送至监测服务器，以通过监测服务器进行信号监测。本发明在保证电子设备整机状态的条件下，实现了机内多路监测信号的实时监测，保证了数据准确测量，同时节约了人力成本，提高了测量效率。 【EN】The invention discloses a system, a method and a device for monitoring electronic equipment signals. The monitoring system of the electronic equipment signal comprises: the monitoring device is arranged in the electronic device, and the monitoring server is connected with the monitoring device; the electronic equipment is internally provided with a test point; the monitoring device is provided with a collection channel, and the collection channel is used for connecting test points in the electronic device. The method for monitoring the electronic equipment signal comprises the following steps: carrying out signal acquisition on each test point in the electronic equipment through an acquisition channel to obtain a corresponding monitoring signal; and analyzing the monitoring signals to obtain corresponding measurement data, and sending the measurement data to a monitoring server so as to monitor the signals through the monitoring server. The invention realizes the real-time monitoring of the multi-path monitoring signals in the electronic equipment under the condition of ensuring the complete machine state of the electronic equipment, ensures the accurate measurement of data, saves the labor cost and improves the measurement efficiency.

摘要：【中文】一种加热炉富氧燃烧安全保护控制方法，具体方法如下：包括三级控制，二级控制启动时先启动一级控制，三级控制启动时先启动一级控制和二级控制；所述一级控制为氧气阀组自身保护系统检测；所述二级控制为氧气阀组与加热炉富氧燃烧系统之间连锁保护监测；所述三级控制为加热炉自身连锁保护；与现有技术相比，本发明的有益效果是：本发明保证富氧燃烧技术在加热炉实现增加产能，节约煤气的同时，富氧燃烧系统安全稳定运行。 【EN】A heating furnace oxygen-enriched combustion safety protection control method comprises the following specific steps: the method comprises three-stage control, wherein the first-stage control is started firstly when the second-stage control is started, and the first-stage control and the second-stage control are started firstly when the third-stage control is started; the primary control is detection of an oxygen valve set self-protection system; the secondary control is linkage protection monitoring between the oxygen valve group and the oxygen-enriched combustion system of the heating furnace; the three-stage control is the self-interlocking protection of the heating furnace; compared with the prior art, the invention has the beneficial effects that: the invention ensures that the oxygen-enriched combustion technology can increase the productivity of the heating furnace and save coal gas, and the oxygen-enriched combustion system can safely and stably operate.