摘要：【中文】一种赤瓟花叶病毒的多克隆抗体制备及ELISA检测方法，所述制备方法包括如下步骤：以赤瓟花叶病毒核酸基因组RNA为模板，基于赤瓟花叶病毒保守CP蛋白核苷酸序列，采用引物ThDMV‑CP1和ThDMV‑CP2进行RT‑PCR扩增，扩增产物经纯化后连接于pET‑30a(+)表达载体上，并对CP蛋白进行表达和纯化，将纯化蛋白与弗氏佐剂混合免疫兔子，采集兔子血液，经亲和层析纯化获得赤瓟花叶病毒多克隆抗体。本发明利用ThDMV病毒制备出相应的特异性多克隆抗体，并建立了具有高特异性和灵敏性的相应的间接ELISA检测病毒方法，具有操作方便、灵敏度高、特异性强、成本低等优势，同时适于大批量样品检测。 【EN】A method for preparing polyclonal antibody of Thladiantha dubia mosaic virus and ELISA detection comprises the following steps: the Thladiantha dubia mosaic virus polyclonal antibody is obtained by taking the RNA of the Thladiantha dubia mosaic virus nucleic acid genome as a template, carrying out RT-PCR amplification by adopting primers ThDMV-CP1 and ThDMV-CP2 based on the conserved CP protein nucleotide sequence of the Thladiantha dubia mosaic virus, purifying and connecting an amplification product to a pET-30a (+) expression vector, expressing and purifying the CP protein, mixing the purified protein with Freund's adjuvant to immunize rabbits, collecting rabbit blood, and purifying by affinity chromatography. The invention utilizes ThDMV virus to prepare corresponding specific polyclonal antibody, establishes a corresponding indirect ELISA virus detection method with high specificity and sensitivity, has the advantages of convenient operation, high sensitivity, strong specificity, low cost and the like, and is suitable for detecting large-batch samples.

摘要：【中文】本发明公开了一种具有对称扇形风门进风口结构的汽车空调，包括左壳体、右壳体、进风风门、风门转轴和风门控制摇臂，所述左壳体、右壳体的前后两侧和上端均设有进风网口，并且左壳体的左端前后两侧设有两个扇形进风网口，所述进风风门装配于左壳体、右壳体之间，风门转轴装配于进风风门的短轴一端部，风门控制摇臂装配于左壳体的圆柱形凸台上，该具有对称扇形风门进风口结构的汽车空调，结构简单，内循环进风口的进风面积远大于单个扇形风门的面积，可以有效减小内循环进风阻力，装配简单且装配效率较高，装配质量有保障，能够降低开发成本，减少劳动量，缩短装配工时，提高生产效率。 【EN】The invention discloses an automobile air conditioner with a symmetrical fan-shaped air door air inlet structure, which comprises a left shell, a right shell, an air inlet door, an air door rotating shaft and an air door control rocker arm, wherein the front side and the rear side and the upper end of the left shell and the right shell are respectively provided with an air inlet net opening, the front side and the rear side of the left end of the left shell are respectively provided with two fan-shaped air inlet net openings, the air inlet door is assembled between the left shell and the right shell, the air door rotating shaft is assembled at one end part of a short shaft of the air inlet door, and the air door control rocker arm is assembled on a cylindrical boss of the left shell. Shortening the assembly time and improving the production efficiency.

摘要：【中文】本发明公开了一种超宽特厚钢质安全壳核电用钢的轧制方法，该方法根据目标钢板规格选择坯料，保证压缩比≥3，保障轧制过程钢板在厚度方向的变形量；采用高温加热坯料并配以合适的均热时间和温度均匀性等工艺参数，实现坯料充分奥氏体化，合金成分均匀化；道次间冷却轧制工艺技术，使得坯料近表层金属表现出更难变形的状态，由表面至心部得到硬度梯度，从而得到心部变形量增大的趋势，获得有效的心部轧制渗透效果。能够成功轧制厚度覆盖60‑101mm，宽度覆盖4000‑4600mm规格的钢板，并保证钢板性能具有强韧性匹配优异，心部性能沿钢板宽度方向均质性良好的特点。 【EN】The invention discloses a rolling method of ultra-wide and ultra-thick steel containment nuclear power steel, which selects a blank according to the specification of a target steel plate, ensures that the compression ratio is more than or equal to 3, and ensures the deformation of the steel plate in the thickness direction in the rolling process; the blank is heated at high temperature and is matched with proper technological parameters such as soaking time, temperature uniformity and the like, so that the full austenitization of the blank is realized, and the alloy components are homogenized; the inter-pass cooling rolling technology enables the metal near the surface layer of the blank to show a more difficult-to-deform state, and obtains a hardness gradient from the surface to the core, thereby obtaining the trend of increasing the deformation of the core and obtaining the effective core rolling infiltration effect. The steel plate with the thickness covering 60-101mm and the width covering 4000-4600mm can be successfully rolled, and the characteristics of excellent obdurability matching and good homogeneity of the core performance along the width direction of the steel plate are ensured.

摘要：【中文】本发明公开了一种超细锗粉粒径控制的方法，所述方法包括以下步骤：将粒径为5um～100um的二氧化锗生产原料投入粉体气流粉碎机进行处理，得到粒径为15um～45um的二氧化锗粉体；将得到的粒径为15um～45um的二氧化锗粉体装入石墨舟中，置于还原炉的石英管内，将进料口堵严密后开动真空泵抽真空，将还原炉内的空气抽尽后开始通入纯净氢气，并开始加热升温，至出气尾管没有水流出时，表示还原反应结束，停止加热，继续通纯净氢气，待炉温慢慢冷却到室温时。本发明对超细锗粉的生产源头二氧化锗的粒径进行控制和处理，将二氧化锗经过粉体气流粉碎机进行粉碎，利用生产过程中粉体物料的团聚，很好的解决了超细锗粉产出小、物料浪费大、能耗高的问题。 【EN】The invention discloses a method for controlling the grain size of superfine germanium powder, which comprises the following steps: putting a germanium dioxide production raw material with the particle size of 5-100 um into a powder jet mill for treatment to obtain germanium dioxide powder with the particle size of 15-45 um; loading the obtained germanium dioxide powder with the particle size of 15-45 um into a graphite boat, placing the graphite boat in a quartz tube of a reduction furnace, starting a vacuum pump to vacuumize after a feed inlet is tightly blocked, starting to introduce pure hydrogen after air in the reduction furnace is completely pumped, starting to heat up until no water flows out from an air outlet tail pipe, indicating that the reduction reaction is finished, stopping heating, continuing to introduce the pure hydrogen, and slowly cooling the furnace to room temperature. The invention controls and processes the grain diameter of germanium dioxide at the production source of the superfine germanium powder, the germanium dioxide is crushed by the powder jet mill, and the problems of small output, large material waste and high energy consumption of the superfine germanium powder are well solved by utilizing the agglomeration of powder materials in the production process.

摘要：【中文】一种锗镜片精密抛光方法，包括以下步骤：S1：将锗镜片放入PFA或PVDF的装夹内；S2：通过磨抛机快速去除锗镜片表面损失层；S3：将锗镜片取下，进行数控抛光精修面型。本发明中，采用高速磨抛与数控精修的一体化抛光加工方法，抛光效率及镜片表面质量得到很大提升，加工表面质量具备一致性，便于实现智能化加工，降低劳动力，提高加工效率和加工质量。 【EN】A germanium lens precision polishing method comprises the following steps: s1: putting the germanium lens into a clamp of PFA or PVDF; s2: rapidly removing a germanium lens surface loss layer by a grinding and polishing machine; s3: and taking off the germanium lens, and carrying out numerical control polishing and finishing to finish the surface shape. According to the invention, by adopting the integrated polishing processing method of high-speed grinding and polishing and numerical control fine trimming, the polishing efficiency and the surface quality of the lens are greatly improved, the processed surface quality is consistent, intelligent processing is conveniently realized, the labor force is reduced, and the processing efficiency and the processing quality are improved.

摘要：【中文】本发明公开了超声波技术在锗系催化剂生产中运用的方法，所述方法包括以下步骤：将40um～60um的超细锗粉、水和分散剂分别投入调浆罐，混匀成为超细锗粉浆料；在研磨机一的研磨腔尾端分离网内安装大功率的超声波发生器一，在研磨机二的研磨腔尾端分离网内安装大功率的超声波发生器二；将超细锗粉浆料放入研磨机一内开始进行第一阶段研磨，同时打开超声波发生器一，研磨30分钟后关闭研磨机一，待超细锗粉浆料冷却至室温后，关闭超声波发生器一。本发明针对超细颗粒产生的团聚，在研磨腔尾端分离网内安装有大功率的超声波发生器，强大的超声波能量可削弱超细颗粒的表面能，可以有效地防止颗粒的团聚，使之充分分散。 【EN】The invention discloses a method for applying ultrasonic technology in the production of a germanium catalyst, which comprises the following steps: respectively putting the superfine germanium powder of 40-60 um, water and a dispersing agent into a slurry mixing tank, and uniformly mixing to obtain superfine germanium powder slurry; a high-power ultrasonic generator I is arranged in a separation net at the tail end of a grinding cavity of the grinding machine I, and a high-power ultrasonic generator II is arranged in a separation net at the tail end of a grinding cavity of the grinding machine II; and (3) placing the superfine germanium powder slurry into a first grinding machine to start the first-stage grinding, simultaneously turning on a first ultrasonic generator, turning off the first grinding machine after grinding for 30 minutes, and turning off the first ultrasonic generator after the superfine germanium powder slurry is cooled to room temperature. Aiming at the agglomeration generated by the ultrafine particles, the invention is provided with the high-power ultrasonic generator in the separation net at the tail end of the grinding cavity, and the strong ultrasonic energy can weaken the surface energy of the ultrafine particles, thereby effectively preventing the agglomeration of the particles and fully dispersing the particles.

摘要：【中文】本发明公开了一种锗泥预处理方法，首先通过过滤除去含有锗泥的设备冷却液和切削液中含有的胶、油、沙及其它有机物或体积较大的无机物，得到含杂质较少的锗泥混合液，然后通过沉降槽多级沉降后，锗泥将在沉降槽内得到统一回收，再将沉降槽内收集的锗泥混合液，经过压滤机渣液分离，得到含水较少的锗泥渣，再将压滤得到的含水锗泥渣，在研磨机内进行湿法研磨，得到粒度更细的锗泥，减少过程的飞扬损失，最后将研磨得到的锗泥，经过烘干脱水得到含水微量、粒度更细的高品位锗泥，作为氯化蒸馏的原料提取四氯化锗。本发明解决了现有的锗泥处理技术在锗泥初期预处理过程中，锗的飞扬损失太过严重，对人体伤害明显，无法满足提取要求的问题。 【EN】The invention discloses a germanium mud pretreatment method, which comprises the steps of firstly filtering to remove glue, oil, sand and other organic matters or inorganic matters with larger volume contained in equipment cooling liquid and cutting liquid containing germanium mud to obtain germanium mud mixed liquid with less impurities, then carrying out multistage sedimentation through a sedimentation tank, uniformly recovering the germanium mud in the sedimentation tank, carrying out slag-liquid separation on the germanium mud mixed liquid collected in the sedimentation tank through a filter press to obtain germanium mud slag with less water, carrying out wet grinding on the water-containing germanium mud slag obtained through filter pressing in a grinder to obtain the germanium mud with finer granularity, reducing flying loss in the process, and finally drying and dehydrating the ground germanium mud to obtain high-grade germanium mud with trace water and finer granularity, wherein the high-grade germanium mud is used as a raw material for chlorination distillation to extract germanium tetrachloride. The invention solves the problems that the flying loss of germanium is too serious, the damage to human body is obvious and the extraction requirement cannot be met in the initial pretreatment process of germanium mud in the existing germanium mud treatment technology.